Redox active molybdophosphate produced by Cu3(PO4)2nanospheres for enhancing enzyme-free electrochemical immunoassay of C-reactive protein

2017 ◽  
Vol 41 (20) ◽  
pp. 11867-11871 ◽  
Author(s):  
Xiaofeng Tan ◽  
Lianhua Zhang ◽  
Xiaobo Deng ◽  
Luyang Miao ◽  
He Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Electrochemical Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Redox Active

Redox-active molybdophosphate produced by Cu3(PO4)2nanospheres has been directly employed for signal amplification of an enzyme-free electrochemical immunosensor.

scholarly journals Feasibility of a Novel Mobile C-Reactive Protein–Testing Device Using Gold-Linked Electrochemical Immunoassay: Clinical Performance Study (Preprint)

2020 ◽  
Author(s):  
Yuko Gondoh-Noda ◽  
Mitsuhiro Kometani ◽  
Akihiro Nomura ◽  
Daisuke Aono ◽  
Shigehiro Karashima ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Older Patients ◽  
Significant Positive Correlation ◽  
Electrochemical Immunoassay ◽  
Testing Device ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Home Based ◽  
Home Based Care

BACKGROUND Home-based care is one of the most promising solutions to provide sufficient medical care for several older patients in Japan. However, because of insufficient diagnostic devices, it is sometimes difficult to detect early signs of the occurrence or worsening of diseases, such as infections under home-based care settings. C-reactive protein (CRP) is highly sensitive to diagnosing infections, and its elevation can help diagnose acute infection in older patients. Therefore, a CRP-measuring device that can be used in such a specific occasion is needed for home-based care. However, aspects such as its size, weight, and procedure are still challenging with respect to the practical use of mobile devices that quantitatively measure CRP levels easily and quickly under home-based care settings. OBJECTIVE We developed a new mobile, rapid CRP measurement device using a gold-linked electrochemical immunoassay (GLEIA) system. The aim of this study was to evaluate the feasibility of this mobile CRP-testing device. METHODS First, we assessed the performance of bare GLEIA-based electrode chips as the foundation of the device. After embedding the bare GLEIA-based electrode chips in a special plastic case and developing the mobile CRP-testing device, we further tested the device prototype using clinical blood samples. Finally, we evaluated the intra-assay variability for precision in the same condition and inter-assay variability for reproducibility in different conditions. RESULTS Blood samples for analysis were obtained by direct vein puncture from outpatients (N=85; females: 57/85; males: 28/85; age: 19-88 years) at Kanazawa University Hospital in Japan. For performance evaluation of bare GLEIA-based electrode chips, we used 85 clinical blood samples. There was a significant positive correlation between the electrode-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.947; <i>P</i>&lt;.001). The assembled device was mobile (size 45×90×2.4 mm; weight 10 g) and disposable. The minimum volume of the sample needed for measuring CRP was 1.4 µL. The estimated preanalytical time was approximately 7 minutes and 40 seconds, and analysis time was approximately 1 minute and 10 seconds. Subsequently, for performance evaluation of the mobile CRP-testing device using GLEIA-based electrode chips, we used 26 clinical blood samples and found a significant positive correlation between the mobile device-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.866, <i>P</i>&lt;.001). The intra-assay variabilities were 34.2%, 40.8%, and 24.5% for low, medium, and high CRP concentrations, respectively. The inter-assay variabilities were 46.5%, 38.3%, and 64.1% for low, medium, and high CRP concentrations, respectively. CONCLUSIONS Our findings suggest that this new mobile CRP-testing device might be suitable for use in home-based care settings.

A novel electrochemical immunoassay for carcinoembryonic antigen based on glucose oxidase-encapsulated nanogold hollow spheres with a pH meter readout

The Analyst ◽  
2018 ◽  
Vol 143 (21) ◽  
pp. 5271-5277 ◽  
Author(s):  
Yu Jiang ◽  
Zhiying Su ◽  
Jian Zhang ◽  
Meijiao Cai ◽  
Lili Wu
Keyword(s):  
Carcinoembryonic Antigen ◽  
Glucose Oxidase ◽  
Signal Amplification ◽  
Hollow Spheres ◽  
Electrochemical Immunosensor ◽  
Hollow Microspheres ◽  
Electrochemical Immunoassay

A portable electrochemical immunosensor was designed for the detection of CEA on a pH meter by using glucose oxidase-encapsulated gold hollow microspheres for signal amplification.

scholarly journals An electrochemical immunosensor coupling a bamboo-like carbon nanostructure substrate with toluidine blue-functionalized Cu(ii)-MOFs as signal probes for a C-reactive protein assay

RSC Advances ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 6699-6708 ◽  
Author(s):  
Mei Li ◽  
Xiaojuan Xia ◽  
Shuang Meng ◽  
YuChan Ma ◽  
Tong Yang ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Metal Organic Framework ◽  
Electrochemical Immunosensor ◽  
C Reactive Protein ◽  
Carbon Nanostructure ◽  
Protein Assay ◽  
Nitrogen Doped ◽  
Reactive Protein ◽  
Metal Organic ◽  
Organic Framework

A sandwich immunosensor based on a toluidine blue (Tb) loaded metal organic framework (Cu(ii)-HKUST-1/Tb) as the signal label and a nitrogen-doped 3D carbon nanostructure as the immobilizing matrix was constructed for the detection of C-reactive protein.

An Electrochemical Immunosensor for Assays of C-Reactive Protein

2003 ◽  
Vol 36 (8) ◽  
pp. 1547-1556 ◽  
Author(s):  
Jun-Jie Zhu ◽  
Jin-Zhong Xu ◽  
Jin-Tao He ◽  
Yi-Jun Wang ◽  
Qiang Miao ◽  
...  
Keyword(s):  
Electrochemical Immunosensor ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Feasibility of a Novel Mobile C-Reactive Protein–Testing Device Using Gold-Linked Electrochemical Immunoassay: Clinical Performance Study

10.2196/18782 ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. e18782
Author(s):  
Yuko Gondoh-Noda ◽  
Mitsuhiro Kometani ◽  
Akihiro Nomura ◽  
Daisuke Aono ◽  
Shigehiro Karashima ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Older Patients ◽  
Significant Positive Correlation ◽  
Electrochemical Immunoassay ◽  
Testing Device ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Home Based ◽  
Home Based Care

Background Home-based care is one of the most promising solutions to provide sufficient medical care for several older patients in Japan. However, because of insufficient diagnostic devices, it is sometimes difficult to detect early signs of the occurrence or worsening of diseases, such as infections under home-based care settings. C-reactive protein (CRP) is highly sensitive to diagnosing infections, and its elevation can help diagnose acute infection in older patients. Therefore, a CRP-measuring device that can be used in such a specific occasion is needed for home-based care. However, aspects such as its size, weight, and procedure are still challenging with respect to the practical use of mobile devices that quantitatively measure CRP levels easily and quickly under home-based care settings. Objective We developed a new mobile, rapid CRP measurement device using a gold-linked electrochemical immunoassay (GLEIA) system. The aim of this study was to evaluate the feasibility of this mobile CRP-testing device. Methods First, we assessed the performance of bare GLEIA-based electrode chips as the foundation of the device. After embedding the bare GLEIA-based electrode chips in a special plastic case and developing the mobile CRP-testing device, we further tested the device prototype using clinical blood samples. Finally, we evaluated the intra-assay variability for precision in the same condition and inter-assay variability for reproducibility in different conditions. Results Blood samples for analysis were obtained by direct vein puncture from outpatients (N=85; females: 57/85; males: 28/85; age: 19-88 years) at Kanazawa University Hospital in Japan. For performance evaluation of bare GLEIA-based electrode chips, we used 85 clinical blood samples. There was a significant positive correlation between the electrode-predicted CRP levels and the reference CRP concentrations (R2=0.947; P<.001). The assembled device was mobile (size 45×90×2.4 mm; weight 10 g) and disposable. The minimum volume of the sample needed for measuring CRP was 1.4 µL. The estimated preanalytical time was approximately 7 minutes and 40 seconds, and analysis time was approximately 1 minute and 10 seconds. Subsequently, for performance evaluation of the mobile CRP-testing device using GLEIA-based electrode chips, we used 26 clinical blood samples and found a significant positive correlation between the mobile device-predicted CRP levels and the reference CRP concentrations (R2=0.866, P<.001). The intra-assay variabilities were 34.2%, 40.8%, and 24.5% for low, medium, and high CRP concentrations, respectively. The inter-assay variabilities were 46.5%, 38.3%, and 64.1% for low, medium, and high CRP concentrations, respectively. Conclusions Our findings suggest that this new mobile CRP-testing device might be suitable for use in home-based care settings.

An ultrasensitive label-free immunoassay for C-reactive protein detection in human serum based on electron transfer

2016 ◽  
Vol 8 (32) ◽  
pp. 6202-6207 ◽  
Author(s):  
Xi Zhang ◽  
Rong Hu ◽  
Kunlei Zhang ◽  
Ruyan Bai ◽  
Delei Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Human Serum ◽  
Protein Detection ◽  
Electrochemical Immunoassay ◽  
Label Free ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein

A rapid, ultrasensitive, and practical label-free electrochemical immunoassay for measuring C-reactive protein (CRP) in real serum samples was developed.

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