An ultrasensitive label-free immunoassay for C-reactive protein detection in human serum based on electron transfer

2016 ◽  
Vol 8 (32) ◽  
pp. 6202-6207 ◽  
Author(s):  
Xi Zhang ◽  
Rong Hu ◽  
Kunlei Zhang ◽  
Ruyan Bai ◽  
Delei Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Human Serum ◽  
Protein Detection ◽  
Electrochemical Immunoassay ◽  
Label Free ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein

A rapid, ultrasensitive, and practical label-free electrochemical immunoassay for measuring C-reactive protein (CRP) in real serum samples was developed.

A simple label-free aptamer-based method for C-reactive protein detection

2016 ◽  
Vol 8 (21) ◽  
pp. 4177-4180 ◽  
Author(s):  
Bin Wu ◽  
Nandi Chen ◽  
Qing Wang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Protein Detection ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein

We developed a simple label-free aptamer-based method for C-reactive protein (CRP) detection.

Fabrication of Electrochemical Biosensor Composed of Multi-functional Ag ions intercalated-DNA 4-Way Junction/Rhodium Nanoplate Heterolayer on Micro-Gap for C-reactive Protein Detection in Human Serum

The Analyst ◽  
2021 ◽  
Author(s):  
Jinmyeong Kim ◽  
Jeong-Ah Park ◽  
Gyeonghye Yim ◽  
Hongje Jang ◽  
Tae-Hyung Kim ◽  
...  
Keyword(s):  
Human Serum ◽  
Acute Coronary Syndromes ◽  
Electrochemical Biosensor ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Coronary Syndromes

As inflammation plays a role in the pathogenesis of acute coronary syndromes, C-reactive protein (CRP) can be used as a biomarker. To detect CRP precisely, authors fabricated a CRP electrochemical...

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

Myocardial Infarction Biomarker C‐reactive Protein Detection on Nanocomposite Aptasensor

2020 ◽  
Author(s):  
Jing Li ◽  
Haitao Li ◽  
Jinpeng Xu ◽  
Xingzhou Zhao ◽  
Shujiang Song ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

Label-free RNA aptamer-based capacitive biosensor for the detection of C-reactive protein

2010 ◽  
Vol 12 (32) ◽  
pp. 9176 ◽  
Author(s):  
Anjum Qureshi ◽  
Yasar Gurbuz ◽  
Saravan Kallempudi ◽  
Javed H. Niazi
Keyword(s):  
Rna Aptamer ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Capacitive Biosensor
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