scholarly journals Label-Free Electrochemical Immunoassay for C-Reactive Protein

Biosensors ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. 34 ◽  
Author(s):  
◽  
◽  
Keyword(s):  
Electrochemical Immunoassay ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein

An ultrasensitive label-free immunoassay for C-reactive protein detection in human serum based on electron transfer

2016 ◽  
Vol 8 (32) ◽  
pp. 6202-6207 ◽  
Author(s):  
Xi Zhang ◽  
Rong Hu ◽  
Kunlei Zhang ◽  
Ruyan Bai ◽  
Delei Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Human Serum ◽  
Protein Detection ◽  
Electrochemical Immunoassay ◽  
Label Free ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein

A rapid, ultrasensitive, and practical label-free electrochemical immunoassay for measuring C-reactive protein (CRP) in real serum samples was developed.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

Label-free RNA aptamer-based capacitive biosensor for the detection of C-reactive protein

2010 ◽  
Vol 12 (32) ◽  
pp. 9176 ◽  
Author(s):  
Anjum Qureshi ◽  
Yasar Gurbuz ◽  
Saravan Kallempudi ◽  
Javed H. Niazi
Keyword(s):  
Rna Aptamer ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Capacitive Biosensor

Label-free detection of C-reactive protein using reflectometric interference spectroscopy-based sensing system

2012 ◽  
Vol 728 ◽  
pp. 64-68 ◽  
Author(s):  
Hyung Woo Choi ◽  
Yasuhiko Sakata ◽  
Yoshikazu Kurihara ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Sensing System ◽  
Reflectometric Interference Spectroscopy ◽  
Label Free Detection

Label-free detection of C-Reactive protein using bioresponsive hydrogel-based surface relief diffraction gratings

2021 ◽  
pp. 113561
Author(s):  
María Isabel Lucío ◽  
Andy Hernández Montoto ◽  
Estrella Fernández ◽  
Sabri Alamri ◽  
Tim Kunze ◽  
...  
Keyword(s):  
Surface Relief ◽  
Diffraction Gratings ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Label Free Detection

scholarly journals Feasibility of a Novel Mobile C-Reactive Protein–Testing Device Using Gold-Linked Electrochemical Immunoassay: Clinical Performance Study (Preprint)

2020 ◽  
Author(s):  
Yuko Gondoh-Noda ◽  
Mitsuhiro Kometani ◽  
Akihiro Nomura ◽  
Daisuke Aono ◽  
Shigehiro Karashima ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Older Patients ◽  
Significant Positive Correlation ◽  
Electrochemical Immunoassay ◽  
Testing Device ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Home Based ◽  
Home Based Care

BACKGROUND Home-based care is one of the most promising solutions to provide sufficient medical care for several older patients in Japan. However, because of insufficient diagnostic devices, it is sometimes difficult to detect early signs of the occurrence or worsening of diseases, such as infections under home-based care settings. C-reactive protein (CRP) is highly sensitive to diagnosing infections, and its elevation can help diagnose acute infection in older patients. Therefore, a CRP-measuring device that can be used in such a specific occasion is needed for home-based care. However, aspects such as its size, weight, and procedure are still challenging with respect to the practical use of mobile devices that quantitatively measure CRP levels easily and quickly under home-based care settings. OBJECTIVE We developed a new mobile, rapid CRP measurement device using a gold-linked electrochemical immunoassay (GLEIA) system. The aim of this study was to evaluate the feasibility of this mobile CRP-testing device. METHODS First, we assessed the performance of bare GLEIA-based electrode chips as the foundation of the device. After embedding the bare GLEIA-based electrode chips in a special plastic case and developing the mobile CRP-testing device, we further tested the device prototype using clinical blood samples. Finally, we evaluated the intra-assay variability for precision in the same condition and inter-assay variability for reproducibility in different conditions. RESULTS Blood samples for analysis were obtained by direct vein puncture from outpatients (N=85; females: 57/85; males: 28/85; age: 19-88 years) at Kanazawa University Hospital in Japan. For performance evaluation of bare GLEIA-based electrode chips, we used 85 clinical blood samples. There was a significant positive correlation between the electrode-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.947; <i>P</i>&lt;.001). The assembled device was mobile (size 45×90×2.4 mm; weight 10 g) and disposable. The minimum volume of the sample needed for measuring CRP was 1.4 µL. The estimated preanalytical time was approximately 7 minutes and 40 seconds, and analysis time was approximately 1 minute and 10 seconds. Subsequently, for performance evaluation of the mobile CRP-testing device using GLEIA-based electrode chips, we used 26 clinical blood samples and found a significant positive correlation between the mobile device-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.866, <i>P</i>&lt;.001). The intra-assay variabilities were 34.2%, 40.8%, and 24.5% for low, medium, and high CRP concentrations, respectively. The inter-assay variabilities were 46.5%, 38.3%, and 64.1% for low, medium, and high CRP concentrations, respectively. CONCLUSIONS Our findings suggest that this new mobile CRP-testing device might be suitable for use in home-based care settings.

Platform based Detection Technologies from Micro scale to Nanoscale

2006 ◽  
Vol 915 ◽  
Author(s):  
Vindhya Kundura ◽  
Sudhaprasanna Kumar Padigi ◽  
Shalini Prasad
Keyword(s):  
Rapid Identification ◽  
Electrical Signal ◽  
Label Free ◽  
C Reactive Protein ◽  
Protein Biomarkers ◽  
Reactive Protein ◽  
Non Invasive ◽  
Binding Event ◽  
High Throughput Detection ◽  
Detection Technologies

AbstractRapid, multiplexed, high throughput detection of proteins is essential for the development of protein biomarkers as sensors. Electrical alignment and detection is a non-invasive, label free technique for rapid identification of bimolecular. We present here a micro fabricated platform based detector for rapidly identifying protein biomarkers present in atherosclerotic plaque for rapid clinical diagnosis of arterial obstruction. This is achieved by electrical assembly of polystyrene beads functionalized with specific antibody receptors (anti-C-reactive protein) .The electrical assembly is achieved using electrophoresis. The polystyrene “bridge” micro structure formed due to electrical assembly aids in the amplification of the antibody-antigen binding event. Antigen (C-reactive protein) at nanogram / ml concentration was detected when binding of the antigen resulted in an amplification of the electrical signal that was measured from the base microelectrode platform. This technique is a demonstration of the application of microscale technology (electrodes) in nanoscale (protein) electrical detection.

Rapid C-reactive protein determination in whole blood with a White Light Reflectance Spectroscopy label-free immunosensor for Point-of-Care applications

2018 ◽  
Vol 260 ◽  
pp. 282-288 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Dimitrios Goustouridis ◽  
Konstantinos Misiakos ◽  
Sotirios Kakabakos ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
White Light ◽  
Whole Blood ◽  
Point Of Care ◽  
Reflectance Spectroscopy ◽  
Label Free ◽  
C Reactive Protein ◽  
Protein Determination ◽  
Reactive Protein ◽  
Light Reflectance

Label-free optical detection of C-reactive protein by nanoimprint lithography-based 2D-photonic crystal film

2016 ◽  
Vol 11 (6) ◽  
pp. 831-837 ◽  
Author(s):  
Tatsuro Endo ◽  
Hiroshi Kajita ◽  
Yukio Kawaguchi ◽  
Terumasa Kosaka ◽  
Toshiyuki Himi
Keyword(s):  
Photonic Crystal ◽  
Nanoimprint Lithography ◽  
Optical Detection ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Crystal Film
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