Cellular uptake of pH/reduction responsive phosphorylcholine micelles

2017 ◽  
Vol 41 (20) ◽  
pp. 11828-11838 ◽  
Author(s):  
Yuanyuan Cai ◽  
Shuai Li ◽  
Mengtan Cai ◽  
Yuanwei Chen ◽  
Xianglin Luo
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Drug Carriers ◽  
Ph Responsive ◽  
Ph Reduction ◽  
The Relationship

We study the relationship between the PDEA content and internalization/intracellular drug release of pH responsive phosphorylcholine micelles as drug carriers.

Dual pH-responsive micelles with both charge-conversional property and hydrophobic–hydrophilic transition for effective cellular uptake and intracellular drug release

2016 ◽  
Vol 7 (12) ◽  
pp. 2202-2208 ◽  
Author(s):  
Shuai Li ◽  
Zhouxiang Zhao ◽  
Wei Wu ◽  
Chunmei Ding ◽  
Jianshu Li
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Cellular Uptake ◽  
Diblock Copolymers ◽  
Effective Drug ◽  
Ph Responsive ◽  
A Charge

Two types of diblock copolymers are synthesized to construct dual pH-responsive micelles with a charge-conversional property for effective drug delivery.

scholarly journals Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells

2019 ◽  
Vol 10 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Azam Akbari ◽  
Azim Akbarzadeh ◽  
Morteza Rafiee Tehrani ◽  
Reza Ahangari Cohan ◽  
Mohsen Chiani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Release ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Breast Cancer Cells ◽  
Drug Carriers ◽  
Diffusion Method ◽  
Diphenyl Tetrazolium Bromide

Purpose: Hydroxyurea (HU) is a well-known chemotherapy drug with several side effects which limit its clinical application. This study was conducted to improve its therapeutic efficiency against breast cancer using liposomes as FDA-approved drug carriers. Methods: PEGylated nanoliposomes-containing HU (NL-HU) were made via a thin-film hydration method, and assessed in terms of zeta potential, size, morphology, release, stability, cellular uptake, and cytotoxicity. The particle size and zeta potential of NL-HU were specified by zeta-sizer. The drug release from liposomes was assessed by dialysis diffusion method. Cellular uptake was evaluated by flow cytometry. The cytotoxicity was designated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) test. Results: The size and zeta value of NL-HU were gotten as 85 nm and -27 mV, respectively. NL-HU were spherical.NL-HU vesicles were detected to be stable for two months. The slow drug release and Weibull kinetic model were obtained. Liposomes considerably enhanced the uptake of HU into BT-474 human breast cancer cells. The cytotoxicity of NL-HU on BT-474 cells was found to be significantly more than that of free HU. Conclusion: The results confirmed these PEGylated nanoliposomes containing drug are potentially suitable against in vitro model of breast cancer.

Synthesis of water-dispersible silicon-containing hydroxyapatite nanoparticles with adjustable degradation rates and their applications as pH-responsive drug carriers

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114852-114858 ◽  
Author(s):  
Kaili Lin ◽  
Na Zhang ◽  
Zhilan Yin ◽  
Yuhui Shen ◽  
Weibin Zhang
Keyword(s):  
Drug Release ◽  
Anticancer Drugs ◽  
Environmentally Friendly ◽  
Drug Carriers ◽  
Hydroxyapatite Nanoparticles ◽  
Ph Responsive ◽  
Water Dispersible ◽  
Degradation Rates ◽  
Ph Dependent ◽  
Environmentally Friendly Method

An environmentally friendly method was developed to synthesize water-dispersible Si-HAp nanoparticles with adjustable degradation rates, high loading capacities for anticancer drugs, and sustained and pH-dependent drug release properties.

scholarly journals Schiff-Linked PEGylated Doxorubicin Prodrug Forming pH-Responsive Nanoparticles With High Drug Loading and Effective Anticancer Therapy

2021 ◽  
Vol 11 ◽  
Author(s):  
Jian Song ◽  
Bingbing Xu ◽  
Hui Yao ◽  
Xiaofang Lu ◽  
Yang Tan ◽  
...  
Keyword(s):  
Drug Release ◽  
Tumor Cells ◽  
Drug Loading ◽  
Drug Carriers ◽  
Anticancer Therapy ◽  
Therapeutic Effects ◽  
Ph Responsive ◽  
High Drug ◽  
High Drug Loading ◽  
Mcf 7

Developing efficacious drug delivery systems for targeted cancer chemotherapy remains a major challenge. Here we demonstrated a kind of pH-responsive PEGylated doxorubicin (DOX) prodrug via the effective esterification and Schiff base reactions, which could self-assemble into the biodegradable micelles in aqueous solutions. Owing to low pH values inside the tumor cells, these PEG-Schiff-DOX nanoparticles exhibited high drug loading ability and pH-responsive drug release behavior within the tumor cells or tissues upon changes in physical and chemical environments, but they displayed good stability at physiological conditions for a long period. CCK-8 assay showed that these PEGylated DOX prodrugs had a similar cytotoxicity to the MCF-7 tumor cells as the free DOX drug. Moreover, this kind of nanoparticle could also encapsulate small DOX drugs with high drug loading, sufficient drug release and enhanced therapeutic effects toward MCF-7 cells, which will be benefited for developing more drug carriers with desirable functions for clinical anticancer therapy.

scholarly journals Design of silk–vaterite microsphere systems as drug carriers with pH-responsive release behavior

2015 ◽  
Vol 3 (42) ◽  
pp. 8314-8320 ◽  
Author(s):  
S. S. Liu ◽  
L. J. Liu ◽  
L. Y. Xiao ◽  
Q. Lu ◽  
H. S. Zhu ◽  
...  
Keyword(s):  
Thermal Treatment ◽  
Drug Release ◽  
Drug Carriers ◽  
Treatment Method ◽  
Release Behavior ◽  
Ph Responsive ◽  
The Stability

A simple thermal treatment method was developed to control the stability of silk–vaterite microspheres and achieve tunable drug release behaviors.

pH-Responsive expandable polycarbonate-doxorubicin conjugate nanoparticles for fast intracellular drug release

2021 ◽  
Author(s):  
Enhui Liang ◽  
Zhihao Guo ◽  
Zhuang Hu ◽  
Zhang-Pei Chen ◽  
Aikebaier Reheman ◽  
...  
Keyword(s):  
Drug Release ◽  
Drug Carriers ◽  
Circulation Time ◽  
Powerful Method ◽  
Ph Responsive ◽  
Anticancer Treatment ◽  
Drug Leakage ◽  
Early Drug

Nanoparticles used as drug carriers with prolonged circulation time, reduced early drug leakage, stimulus triggered and improved drug release is a powerful method for anticancer treatment. Herein, a new tailor-made...

Nanostructures for pH-sensitive Drug Delivery and Magnetic Resonance Contrast Enhancement Systems

2018 ◽  
Vol 25 (25) ◽  
pp. 3036-3057 ◽  
Author(s):  
Xiao Sun ◽  
Guilong Zhang ◽  
Zhengyan Wu
Keyword(s):  
Drug Delivery ◽  
Magnetic Resonance ◽  
Drug Release ◽  
Contrast Enhancement ◽  
General Strategy ◽  
Ph Responsive ◽  
Physicochemical Changes ◽  
Magnetic Resonance Contrast ◽  
Resonance Contrast ◽  
Magnetic Resonance Contrast Enhancement

According to the differences of microenvironments between tumors and healthy tissues, if the anticancer drugs or magnetic resonance contrast agents (MRCAs) can be controlled to precisely match physiological needs at targeted tumor sites, it is expected to acquire better therapeutic efficacy and more accurate diagnosis. Over the decade, stimuli-responsive nanomaterials have been a research hotspot for cancer treatment and diagnosis because they show many excellent functions, such as in vivo imaging, combined targeting drug delivery and systemic controlled release, extended circulation time, etc. Among the various stimuli nanosystems, pH-stimuli mode is regarded as the most general strategy because of solid tumors acidosis. When exposed to weakly acidic tumor microenvironment, pH-responsive nanoplatforms can generate physicochemical changes for their structure and surface characteristics, causing drug release or contrast enhancement. In this review, we focused on the designs of various pH-responsive nanoplatforms and discussed the mechanisms of controlled drug release or switch on-off in MRCAs. This review also discussed the efficacy of cellular internalization for these nanoplatforms via endocytosis of acidic tumor cell. Meanwhile, nanoplatforms response to acidic intracellular pH (such as endosome, lysosome) are discussed, along with approaches for improving drug release performance and magnetic resonance contrast enhancement. A greater understanding of these pH-responsive nanoplatforms will help design more efficient nanomedicine to address the challenges encountered in conventional diagnosis and chemotherapy.

scholarly journals Quantum Dots as a Good Carriers of Unsymmetrical Bisacridines for Modulating Cellular Uptake and the Biological Response in Lung and Colon Cancer Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 462 ◽  
Author(s):  
Joanna Pilch ◽  
Patrycja Kowalik ◽  
Piotr Bujak ◽  
Anna M. Nowicka ◽  
Ewa Augustin
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Laser Scanning ◽  
Biological Response ◽  
Drug Carriers ◽  
Confocal Laser ◽  
Normal Cells ◽  
H460 Cells ◽  
Hct116 Cells

Nanotechnology-based drug delivery provides a promising area for improving the efficacy of cancer treatments. Therefore, we investigate the potential of using quantum dots (QDs) as drug carriers for antitumor unsymmetrical bisacridine derivatives (UAs) to cancer cells. We examine the influence of QD–UA hybrids on the cellular uptake, internalization (Confocal Laser Scanning Microscope), and the biological response (flow cytometry and light microscopy) in lung H460 and colon HCT116 cancer cells. We show the time-dependent cellular uptake of QD–UA hybrids, which were more efficiently retained inside the cells compared to UAs alone, especially in H460 cells, which could be due to multiple endocytosis pathways. In contrast, in HCT116 cells, the hybrids were taken up only by one endocytosis mechanism. Both UAs and their hybrids induced apoptosis in H460 and HCT116 cells (to a greater extent in H460). Cells which did not die underwent senescence more efficiently following QDs–UAs treatment, compared to UAs alone. Cellular senescence was not observed in HCT116 cells following treatment with both UAs and their hybrids. Importantly, QDgreen/red themselves did not provoke toxic responses in cancer or normal cells. In conclusion, QDs are good candidates for targeted UA delivery carriers to cancer cells while protecting normal cells from toxic drug activities.

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