Stabilization of the Tight Junction of the Intestinal Caco-2 Cell Monolayer by Milk Whey Proteins

1995 ◽  
Vol 59 (10) ◽  
pp. 1951-1952 ◽  
Author(s):  
Kei Hashimoto ◽  
Kyoko Takeda ◽  
Tsutomu Nakayama ◽  
Makoto Shimizu
2011 ◽  
Vol 10 (4) ◽  
pp. 1746-1754 ◽  
Author(s):  
Yalin Liao ◽  
Rudy Alvarado ◽  
Brett Phinney ◽  
Bo Lönnerdal

1969 ◽  
Vol 11 (3) ◽  
pp. 337-343 ◽  
Author(s):  
F. J. Bourne

SUMMARYA dramatic fall in the colostral whey proteins of nine sows occurred within 24 hr following the birth of the first piglet. The mean levels fell from 19·6 g/100 ml to 4·1 g/100 ml, 50% of this fall occurring within 4 to 6 hr. It is suggested that as early-born piglets will be able to obtain colostrum of a much higher protein concentration this may give them some physiological and immunological advantages over later-born littermates.


2017 ◽  
Vol 18 (4) ◽  
pp. 721 ◽  
Author(s):  
Hicham Benabdelkamel ◽  
Afshan Masood ◽  
Ibrahim Alanazi ◽  
Dunia Alzahrani ◽  
Deema Alrabiah ◽  
...  

2001 ◽  
Vol 280 (5) ◽  
pp. G910-G921 ◽  
Author(s):  
Frederic Hollande ◽  
Emmanuelle M. Blanc ◽  
Jean Pierre Bali ◽  
Robert H. Whitehead ◽  
Andre Pelegrin ◽  
...  

The regulation of intercellular adhesion by hepatocyte growth factor (HGF) was examined on a novel nontumorigenic gastric epithelial cell line (IMGE-5) derived from H-2Kb-tsA58 transgenic mice. IMGE-5 cells constitutively expressed cytokeratin 18 and HGF receptors. Under permissive conditions (33°C + interferon-γ), IMGE-5 cells proliferated rapidly but did not display membrane expression of adherens and tight junction proteins. Under nonpermissive conditions, their proliferation was decreased and they displayed a strong, localized membrane expression of E-cadherin/β-catenin and occludin/ZO-1. HGF treatment largely prevented the targeting of ZO-1 to the tight junction and induced a significant decrease of the transepithelial resistance measured across a confluent IMGE-5 cell monolayer. HGF rapidly increased the tyrosine phosphorylation of ZO-1 and decreased its association with occludin in a phosphatidylinositol 3-kinase (PI 3-kinase)-dependent manner. PI 3-kinase was also involved in HGF-induced migration of IMGE-5 cells. Our results demonstrate that 1) HGF prevents the appearance of ZO-1 in the membrane during epithelial cell differentiation; 2) HGF causes partial relocalization of ZO-1 to the cytoplasm and nucleus and concomitantly stimulates cell dissociation and migration; and 3) IMGE-5 cells offer a useful model for the study of gastric epithelial cell differentiation.


2012 ◽  
pp. 35-63 ◽  
Author(s):  
Dragana Stanic ◽  
Jelena Radosavljevic ◽  
Marija Stojadinovic ◽  
Tanja Cirkovic Velickovic

2008 ◽  
Vol 43 (7) ◽  
pp. 788-794 ◽  
Author(s):  
Cony Gauche ◽  
Joana T.C. Vieira ◽  
Paulo J. Ogliari ◽  
Marilde T. Bordignon-Luiz
Keyword(s):  

1974 ◽  
Vol 37 (5) ◽  
pp. 244-249 ◽  
Author(s):  
C. J. Washam ◽  
G. W. Reinbold ◽  
E. R. Vedamuthu ◽  
R. Jorgensen

Milk proteins were subjected to treatment with various levels of benzoyl peroxide, with and without heating at 60 C for 2 h. Heating had a pronounced effect on whey proteins, but polyacrylamide gel electrophoresis revealed changes in proteins not attributable to heat alone. The effect on proteins was reflected in an increased tendency for the benzoyl peroxide-heat treated cheeses to expel moisture during leakage tests. Use of 17.8 ppm benzoyl peroxide resulted in a markedly whiter cheese than that made using 5.9 ppm and reflectance studies indicated this to be true even when no heat treatment accompanied the benzoyl peroxide. Use of benzoyl peroxide in the bleaching process did not decrease mold development in ripening loaves nor was acid production by lactic cultures diminished. In addition, proteolysis of milk proteins by rennet was not reduced by the presence of benzoyl peroxide.


1969 ◽  
Vol 11 (3) ◽  
pp. 345-349 ◽  
Author(s):  
F. J. Bourne

SUMMARYColostral whey protein levels do not change during the first 4 hr from the start of parturition when suckling is prevented. Both early and late-born piglets returned to the sow at the end of this period are able to suck colostrum of a high protein concentration. Removal of piglets from the sow and the presence of an attendant did not affect the farrowing process.


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