scholarly journals Highly Sensitive and Reliable microRNA Detection with a Recyclable Microfluidic Device and an Easily Assembled SERS Substrate

ACS Omega ◽  
2021 ◽  
Author(s):  
Taeksu Lee ◽  
Soongeun Kwon ◽  
Hak-Jong Choi ◽  
Hyungjun Lim ◽  
Jaejong Lee
Keyword(s):  
Microfluidic Device ◽  
Sers Substrate ◽  
Microrna Detection ◽  
Highly Sensitive

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

scholarly journals Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device

2020 ◽  
Vol 92 (8) ◽  
pp. 5750-5755 ◽  
Author(s):  
Hyewon Lee ◽  
Jiseok Lee ◽  
Seung-Goo Lee ◽  
Patrick S. Doyle
Keyword(s):  
Microfluidic Device ◽  
Colorimetric Assay ◽  
Microrna Detection

A disulfur ligand stabilization approach to construct a silver(i)-cluster-based porous framework as a sensitive SERS substrate

Nanoscale ◽  
2019 ◽  
Vol 11 (35) ◽  
pp. 16293-16298 ◽  
Author(s):  
Tong Wu ◽  
Di Yin ◽  
Xun Hu ◽  
Bo Yang ◽  
Hong Liu ◽  
...  
Keyword(s):  
Hot Spots ◽  
Sers Substrate ◽  
Porosity Structure ◽  
Porous Framework ◽  
Highly Sensitive ◽  
Ligand Stabilization

An atomic-precise silver(i)-cluster-based MOF (UJN-1) stabilized by disulfur ligand has been demonstrated. Its reduced derivative exhibits highly sensitive SERS activity that can be ascribed to abundant hot spots sites and porosity structure.

scholarly journals In-Situ Grown Silver Nanoparticles on Nonwoven Fabrics Based on Mussel-Inspired Polydopamine for Highly Sensitive SERS Carbaryl Pesticides Detection

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 384 ◽  
Author(s):  
Zhiliang Zhang ◽  
Tiantian Si ◽  
Jun Liu ◽  
Guowei Zhou
Keyword(s):  
Silver Nanoparticles ◽  
Collection Efficiency ◽  
High Sensitivity ◽  
Sers Substrate ◽  
Analytical Sensitivity ◽  
Sers Substrates ◽  
Rapid Sampling ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

The rapid sampling and efficient collection of target molecules from a real-world surface is fairly crucial for surface-enhanced Raman scattering (SERS) to detect trace pesticide residues in the environment and in agriculture fields. In this work, a versatile approach was exploited to fabricate a flexible SERS substrate for highly sensitive detection of carbaryl pesticides, using in-situ grown silver nanoparticles (AgNPs)on non-woven (NW) fabric surfaces based on mussel-inspired polydopamine (PDA) molecules. The obtained NW@PDA@AgNPs fabrics showed extremely sensitive and reproducible SERS signals toward crystal violet (CV) molecules, and the detection limit was as low as 1.0 × 10−12 M. More importantly, these NW@PDA@AgNPs fabrics could be directly utilized as flexible SERS substrates for the rapid extraction and detection of trace carbaryl pesticides from various fruit surfaces through a simple swabbing approach. It was identified that the detection limits of carbaryl residues from apple, orange, and banana surfaces were approximately decreased to 4.02 × 10−12, 6.04 × 10−12, and 5.03 × 10−12 g, respectively, demonstrating high sensitivity and superior reliability. These flexible substrates could not only drastically increase the collection efficiency from multifarious irregular-shaped matrices, but also greatly enhance analytical sensitivity and reliability for carbaryl pesticides. The fabricated flexible and multifunctional SERS substrates would have great potential to trace pesticide residue detection in the environment and bioscience fields.

Core/shell Ag@silicate nanoplatelets and poly(vinyl alcohol) spherical nanohybrids fabricated by coaxial electrospraying as highly sensitive SERS substrates

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 67204-67211 ◽  
Author(s):  
Chih-Wei Chiu ◽  
Po-Hsien Lin
Keyword(s):  
Vinyl Alcohol ◽  
Large Scale ◽  
High Efficiency ◽  
Poly Vinyl Alcohol ◽  
Core Shell ◽  
Sers Substrate ◽  
Sers Substrates ◽  
Highly Sensitive ◽  
Coaxial Electrospray

A novel flexible, freestanding, large-scale, and disposable SERS substrate of core/shell Ag@silicate and poly(vinyl alcohol) spherical nanohybrids, fabricated by coaxial electrospray, allows for the high-efficiency detection of adenine from DNA.

Self-assembly of various silver nanocrystals on PmPD/PAN nanofibers as a high-performance 3D SERS substrate

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5707-5715 ◽  
Author(s):  
Peng Jia ◽  
Bing Cao ◽  
Jianqiang Wang ◽  
Jin Qu ◽  
Yuxuan Liu ◽  
...  
Keyword(s):  
Self Assembly ◽  
High Performance ◽  
Sers Substrate ◽  
Sers Substrates ◽  
Highly Sensitive ◽  
Nanofiber Mats ◽  
Pan Nanofiber

The AgNCs (AgNPs, AgNTs and AgNDs) decorated-PmPD/PAN nanofiber mats were obtained as highly sensitive 3D SERS substrates.

Highly sensitive and scalable AAO-based nano-fibre SERS substrate for sensing application

2017 ◽  
Vol 28 (23) ◽  
pp. 235302 ◽  
Author(s):  
L K Lim ◽  
B K Ng ◽  
C Y Fu ◽  
Landobasa Y M Tobing ◽  
D H Zhang
Keyword(s):  
Sers Substrate ◽  
Highly Sensitive ◽  
Sensing Application
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