scholarly journals Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification

Lab on a Chip ◽  
2017 ◽  
Vol 17 (17) ◽  
pp. 2951-2959 ◽  
Author(s):  
James H. Wade ◽  
Joshua D. Jones ◽  
Ivan L. Lenov ◽  
Colleen M. Riordan ◽  
Stephen G. Sligar ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Model Membrane ◽  
Microfluidic Platform ◽  
Membrane Interfaces ◽  
Protein Incorporation

A microfluidic platform for Nanodisc formation and membrane protein incorporation will enable studies of interactions at model membrane interfaces.

scholarly journals Computer simulation of the phase behavior of a model membrane protein: Annexin V

2002 ◽  
Vol 116 (16) ◽  
pp. 7217-7224 ◽  
Author(s):  
Martin A. Bates ◽  
Massimo G. Noro ◽  
Daan Frenkel
Keyword(s):  
Computer Simulation ◽  
Membrane Protein ◽  
Phase Behavior ◽  
Annexin V ◽  
Model Membrane

Mechanisms of antimicrobial peptide action: Studies of indolicidin assembly at model membrane interfaces by in situ atomic force microscopy

2006 ◽  
Vol 154 (1) ◽  
pp. 42-58 ◽  
Author(s):  
James E. Shaw ◽  
Jean-René Alattia ◽  
Jocelyne E. Verity ◽  
Gilbert G. Privé ◽  
Christopher M. Yip
Keyword(s):  
Atomic Force Microscopy ◽  
Antimicrobial Peptide ◽  
Model Membrane ◽  
Force Microscopy ◽  
Atomic Force ◽  
Membrane Interfaces

Interaction of drugs with a model membrane protein

1982 ◽  
Vol 31 (4) ◽  
pp. 527-534 ◽  
Author(s):  
Michael A. Singer
Keyword(s):  
Membrane Protein ◽  
Model Membrane

scholarly journals Assembly of Model Membrane Nanodiscs for Native Mass Spectrometry

2021 ◽  
Author(s):  
Marius Kostelic ◽  
Ciara K. Zak ◽  
Hiruni Jayasekera ◽  
Michael Marty
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Lipid Bilayers ◽  
Biological Membranes ◽  
Native Mass Spectrometry ◽  
Model Membrane ◽  
Peptide Interactions ◽  
Lipid Nanodiscs ◽  
Natural Lipid ◽  
Selection Of

Native mass spectrometry (MS) with nanodiscs is a promising technique for characterizing membrane protein and peptide interactions in lipid bilayers. However, prior studies have used nanodiscs made of only one or two lipids, which lack the complexity of a natural lipid bilayer. To better model specific biological membranes, we developed model mammalian, bacterial, and mitochondrial nanodiscs with up to four different phospholipids. Careful selection of lipids with similar masses that balance the fluidity and curvature enabled these complex nanodiscs to be assembled and resolved with native MS. We then applied this approach to characterize the specificity and incorporation of LL-37, a human antimicrobial peptide, in single lipid nanodiscs versus model bacterial nanodiscs. Overall, development of these model membrane nanodiscs reveals new insights into the assembly of complex nanodiscs and provides a useful toolkit for studying membrane protein, peptide, and lipid interactions in model biological membranes.

Interaction of drugs with a model membrane protein

1980 ◽  
Vol 29 (19) ◽  
pp. 2651-2655 ◽  
Author(s):  
Michael A. Singer
Keyword(s):  
Membrane Protein ◽  
Model Membrane

scholarly journals Hydrophobic Characteristic Is Energetically Preferred for Cysteine in a Model Membrane Protein

2019 ◽  
Vol 117 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Bharat Ramasubramanian Iyer ◽  
Radhakrishnan Mahalakshmi
Keyword(s):  
Membrane Protein ◽  
Model Membrane

scholarly journals Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles

2021 ◽  
Author(s):  
Nicolas Dolder ◽  
Philipp Mueller ◽  
Christoph von Ballmoos
Keyword(s):  
Membrane Proteins ◽  
Model Membrane ◽  
Giant Unilamellar Vesicles ◽  
Cellular Functions ◽  
Unilamellar Vesicles ◽  
Synthetic Cells ◽  
Buffer Composition ◽  
Protein Incorporation ◽  
Functional Investigation

Giant unilamellar vesicles (GUVs) are micrometer-sized model membrane systems that can be viewed directly under the microscope. They serve as scaffolds for the bottom-up creation of synthetic cells, targeted drug delivery and have been used in many in vitro studies of membrane related phenomena. GUVs are also of interest for the functional investigation of membrane proteins that carry out many key cellular functions. A major hurdle to a wider application of GUVs in this field is the diversity of existing protocols that are optimized for individual proteins. Here, we compare PVA assisted and electroformation techniques for GUV formation under physiologically relevant conditions, and analyze the effect of immobilization on vesicle structure and membrane tightness towards small substrates and protons. There, differences in terms of yield, size, and leakage of GUVs produced by PVA assisted swelling and electroformation were found, dependent on salt and buffer composition. Using fusion of oppositely charged membranes to reconstitute a model membrane protein, we find that empty vesicles and proteoliposomes show similar fusion behavior, which allows for a rapid estimation of protein incorporation using fluorescent lipids.

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