Label-free, high-throughput holographic screening and enumeration of tumor cells in blood

Lab on a Chip ◽  
2017 ◽  
Vol 17 (17) ◽  
pp. 2920-2932 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Cell Size ◽  
High Throughput ◽  
Digital Holography ◽  
Label Free

We developed a microfluidic inline digital holography microscopy platform to fingerprint tumor cells in blood based on cell size and optical signatures.

scholarly journals Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo

2020 ◽  
Vol 25 (03) ◽  
pp. 1 ◽  
Author(s):  
Pengfei Hai ◽  
Yuan Qu ◽  
Yang Li ◽  
Liren Zhu ◽  
Leonid Shmuylovich ◽  
...  
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Photoacoustic Tomography ◽  
Label Free ◽  
Melanoma Tumor

scholarly journals High-Throughput Microfluidic Labyrinth for the Label-free Isolation of Circulating Tumor Cells

Cell Systems ◽  
2017 ◽  
Vol 5 (3) ◽  
pp. 295-304.e4 ◽  
Author(s):  
Eric Lin ◽  
Lianette Rivera-Báez ◽  
Shamileh Fouladdel ◽  
Hyeun Joong Yoon ◽  
Stephanie Guthrie ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Label Free

scholarly journals 1204 Label-free high-throughput photoacoustic imaging of circulating melanoma tumor cells in patients in vivo

2018 ◽  
Vol 138 (5) ◽  
pp. S204
Author(s):  
P. Hai ◽  
L. Shmuylovich ◽  
Y. Qu ◽  
L.A. Cornelius ◽  
L. Wang
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Photoacoustic Imaging ◽  
Label Free ◽  
Melanoma Tumor

Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3436-3445 ◽  
Author(s):  
Fei Tian ◽  
Lili Cai ◽  
Jianqiao Chang ◽  
Shanshan Li ◽  
Chao Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Whole Blood ◽  
Rare Tumor ◽  
Label Free ◽  
Size Dependent

Label-free, size-dependent, and high-throughput isolation of rare tumor cells from untreated whole blood is enabled by interfacial viscoelastic microfluidics.

On the Design of a DLD-DEP Device for Separation of Circulating Tumor Cells in Blood

2020 ◽  
Author(s):  
Mehdi Rahmati ◽  
Xiaolin Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Progression ◽  
High Throughput ◽  
Blood Cells ◽  
Lateral Displacement ◽  
Uniform Electric Field ◽  
Label Free ◽  
Particle Tracing ◽  
Key Factor

Abstract Circulating Tumor Cells (CTCs), which migrate from original sites in a body to distant organs through blood, are a key factor in cancer detection. Emerging Label-free techniques owing to their inherent advantage to preserve characteristics of sorted cells and low consumption of samples can be promising to the prediction of cancer progression and metastasis research. Deterministic Lateral Displacement (DLD) is one of the label-free separation techniques employing a specific arrangement of micro-posts for continuous separation of suspended cells in a buffer based on the size of cells. Separation based solely on size is challenging since the size distributions of CTCs might overlap with those of normal blood cells. To address this problem, DLD can be combined with dielectrophoresis (DEP) technique which is the phenomenon of particle movement in a non-uniform electric field owing to the polarization effect. Although, DLD devices employ the laminar flow in low Reynolds number (Re) fluid flow due to predictability of such flow regimes, they should be improved to work in higher Re flow regime so as to attain high throughput devices. In this paper, a particle tracing simulation is developed to study the effects of different post shapes, shift fraction of micropost arrays, and dielectrophoresis forces on separation of CTCs from peripheral blood cells. Our numerical model and results provide a groundwork for design and fabrication of high-throughput DLD-DEP devices for improvement of CTC separation.

Enhancing the Cell Viability in High Throughput Deterministic Lateral Displacement Separation of Circulating Tumor Cells

2019 ◽  
Author(s):  
Arian Aghilinejad ◽  
Christopher Landry ◽  
George Cha ◽  
Xiaolin Chen
Keyword(s):  
Reynolds Number ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Cancer Metastasis ◽  
Lateral Displacement ◽  
Shear Stresses ◽  
Label Free ◽  
Deterministic Lateral Displacement ◽  
Reynolds Number Flow

Abstract Cancer is among a major health concerns all over the world. Cancer metastasis, which defines as the migration of malignant cells from original sites to distant organs, is the main reason of death due to cancer and there is growing evidence that Circulating Tumor Cells (CTCs) are responsible for initiating the metastasis. Due to the importance of these bioparticles in biotechnology and medicine, there is a growing interest to study and separate them through different techniques especially microfluidic label-free technologies. One such technology, termed Deterministic Lateral Displacement (DLD) has recently shown promising abilities to separate cells and particles of different sizes. However, DLD is a separation method that takes advantages of the predictable flow laminae of low Reynolds number (Re) fluid flow. In order to achieve higher device throughput, effects of higher Reynolds number flow on DLD device should be studied. Additionally, the higher flow rates would apply higher forces and shear stresses on the cells which threaten the cell’s viability. In this study, employing numerical simulation, the effect of high Re number on DLD device for separating cancer cells has been investigated. Specifically, we conducted force analysis and by focusing on the downstream gap distance between the posts, we improved the device which results in less cell deformation. Our developed numerical model and presented results lay the groundwork for design and fabrication of high-throughput DLD microchips for enhanced separation of CTCs.

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