Nutritional requirements and the impact of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus

2017 ◽  
Vol 19 (19) ◽  
pp. 4633-4641 ◽  
Author(s):  
Silvia Klotz ◽  
Anja Kuenz ◽  
Ulf Prüße
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Nutritional Requirements ◽  
The Impact ◽  
First Time

For the first time, nutritional requirements including the effects of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus are presented.

scholarly journals Multi-product biorefinery from Arthrospira platensis biomass as feedstock for bioethanol and lactic acid production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Diego A. Esquivel-Hernández ◽  
Anna Pennacchio ◽  
Mario A. Torres-Acosta ◽  
Roberto Parra-Saldívar ◽  
Luciana Porto de Souza Vandenberghe ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Production Cost ◽  
Lactic Acid Production ◽  
Arthrospira Platensis ◽  
Production Costs ◽  
Bioactive Metabolites ◽  
Fermentation Time ◽  
Product Titer ◽  
The Impact

AbstractWith the aim to reach the maximum recovery of bulk and specialty bioproducts while minimizing waste generation, a multi-product biorefinery for ethanol and lactic acid production from the biomass of cyanobacterium Arthrospira platensis was investigated. Therefore, the residual biomass resulting from different pretreatments consisting of supercritical fluid extraction (SF) and microwave assisted extraction with non-polar (MN) and polar solvents (MP), previously applied on A. platensis to extract bioactive metabolites, was further valorized. In particular, it was used as a substrate for fermentation with Saccharomyces cerevisiae LPB-287 and Lactobacillus acidophilus ATCC 43121 to produce bioethanol (BE) and lactic acid (LA), respectively. The maximum concentrations achieved were 3.02 ± 0.07 g/L of BE by the MN process at 120 rpm 30 °C, and 9.67 ± 0.05 g/L of LA by the SF process at 120 rpm 37 °C. An economic analysis of BE and LA production was carried out to elucidate the impact of fermentation scale, fermenter costs, production titer, fermentation time and cyanobacterial biomass production cost. The results indicated that the critical variables are fermenter scale, equipment cost, and product titer; time process was analyzed but was not critical. As scale increased, costs tended to stabilize, but also more product was generated, which causes production costs per unit of product to sharply decrease. The median value of production cost was US$ 1.27 and US$ 0.39, for BE and LA, respectively, supporting the concept of cyanobacterium biomass being used for fermentation and subsequent extraction to obtain ethanol and lactic acid as end products from A. platensis.

scholarly journals Impact of Hydrolysis Methods on the Utilization of Agricultural Residues as Nutrient Source for D-lactic Acid Production by Sporolactobacillus inulinus

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 12 ◽  
Author(s):  
Silvia Brock ◽  
Anja Kuenz ◽  
Ulf Prüße
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Industrial Process ◽  
Rapeseed Meal ◽  
Agricultural Residues ◽  
Nutrient Source ◽  
Nutrient Sources ◽  
Cheap Alternative

d-lactic acid is a building block for heat resistant polylactic acid, a biobased polymer with a high potential. Nevertheless, an economically efficient industrial process for d-lactic acid production still needs to be implemented. Yeast extract is an expensive nutrient source, which is used to fulfill the complex nutritional requirements in lactic acid fermentations. The substitution of yeast extract by cheap alternative nutrient sources is a challenge in many fermentation processes. In this study, chemical and enzymatic hydrolysis techniques for protein rich agricultural residues and their effectiveness are compared, as well as their impact on the d-lactic acid production of Sporolactobacillus inulinus. An efficient substitution of yeast extract could be achieved by a variety of agricultural residues, hydrolysed with 3M H2SO4, demonstrating the much higher versatility and effectiveness of this method compared to enzymatic methods. In a fed-batch experiment with chemically hydrolyzed rapeseed meal and minimal supplementation, a lactic acid titer of 221 g L−1 and an overall productivity of 1.55 g (L h)−1 (96% yield) were obtained.

scholarly journals Selection of the StrainLactobacillus acidophilusATCC 43121 and Its Application to Brewers’ Spent Grain Conversion into Lactic Acid

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Rossana Liguori ◽  
Carlos Ricardo Soccol ◽  
Luciana Porto de Souza Vandenberghe ◽  
Adenise Lorenci Woiciechowski ◽  
Elena Ionata ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Synthetic Medium ◽  
Glucose Consumption ◽  
Acid Yield ◽  
Aqueous Ammonia Soaking ◽  
Spent Grain ◽  
Selection Of

SixLactobacillusstrains were analyzed to select a bacterium for conversion of brewers’ spent grain (BSG) into lactic acid. Among the investigated strains,L. acidophilusATCC 43121 showed the highest yield of lactic acid production (16.1 g/L after 48 hours) when grown in a synthetic medium. It was then analyzed for its ability to grow on the hydrolysates obtained from BSG after acid-alkaline (AAT) or aqueous ammonia soaking (AAS) pretreatment. The lactic acid production byL. acidophilusATCC 43121 through fermentation of the hydrolysate from AAS treated BSG was 96% higher than that from the AAT treated one, although similar yields of lactic acid per consumed glucose were achieved due to a higher (46%) glucose consumption byL. acidophilusATCC 43121 in the AAS BSG hydrolysate. It is worth noting that adding yeast extract to the BSG hydrolysates increased both the yield of lactic acid per substrate consumed and the volumetric productivity. The best results were obtained by fermentation of AAS BSG hydrolysate supplemented by yeast extract, in which the strain produced 22.16 g/L of lactic acid (yield of 0.61 g/g), 27% higher than the value (17.49 g/L) obtained in the absence of a nitrogen source.

scholarly journals Hydrolyzed Agricultural Residues—Low-Cost Nutrient Sources for l-Lactic Acid Production

Fermentation ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 97 ◽  
Author(s):  
Susan Krull ◽  
Silvia Brock ◽  
Ulf Prüße ◽  
Anja Kuenz
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Rapeseed Meal ◽  
Agricultural Residues ◽  
Nutrient Sources ◽  
Protein Rich

Lactic acid is a building block for polylactic acid, which is one of the most promising polymers based on renewable resources and is used mainly in packaging industry. This bio-based polymer is biodegradable and provides an ecological and economical alternative to petrochemical plastics. The largest cost blocks of biotechnological lactic acid production, accounting for up to 38% of the total costs, are substrate and nutrient sources, such as peptone, meat, and yeast extract. Based on a systematic analysis of nutritional requirements, the substitution of yeast extract by low-cost protein-rich agricultural hydrolysates was estimated for the production of l-lactic acid with Lactobacillus casei. Cultivations in 24-well microtiter plates enabled analysis of nutrient requirements and the usage of various hydrolysates with a high parallel throughput and repeated sampling. Rapeseed meal (RM) and distillers’ dried grains with solubles (DDGS) were tested as low-cost protein-rich agricultural residues. By using chemically or enzymatically hydrolyzed rapeseed meal or DDGS, 70% of the nutrient sources was replaced in the fermentation process at identical productivity and product yields. All in all, the total costs of l-lactic acid production with Lactobacillus casei could potentially be reduced by up to 23%.

scholarly journals Statistical Optimization of Lactic Acid Production by Lactobacillus pentosus using Hemicellulosic Hydrolysate from Sugarcane Bagasse

2018 ◽  
Vol 29 (1) ◽  
pp. 41-51
Author(s):  
Daiana Wischral
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Hemicellulose Hydrolysate ◽  
Anaerobic Conditions ◽  
Lactobacillus Pentosus

Lactic acid, traditionally obtained through fermentation processes, presents numerous applications in the chemical industry. Among these is the production of polymers, more specifically biodegradable polylactic acid (PLA). Development of processes that use low cost substrates, such as bioproduction of lactic acid, could improve the economic viability of bioprocesses. Thus, the present work reports investigation of hemicellulose hydrolysate from sugarcane bagasse as a sole carbon source for lactic acid production by Lactobacillus pentosus ATCC 8041. Initially, sugarcane bagasse was pretreated with acid in a solid:liquid ratio of 1:2.8 (1 g of bagasse: 2.8 mL of sulfuric acid solution 1 % v/v) and at a temperature of 121°C for 27 minutes. Then, concentration of both the hemicellulose hydrolysate and the yeast extract in MRS medium were optimized usingResponse Surface Methodology through software STATISTICA 6.0. Once the optimal conditions (40 % of hemicellulose hydrolysate and 5 g/L of yeast extract) were validated, fermentations were carried out in anaerobic conditions at 37°C and 120 rpm. After 48h, 19.17 g/L of lactic acid were produced, corresponding to a volumetric productivity of 0.40 g/L.h1. Findings of this work demonstrate that hemicellulose hydrolysate from sugarcane bagasse is a promising carbon source for lactic acid production.

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