scholarly journals Selection of the StrainLactobacillus acidophilusATCC 43121 and Its Application to Brewers’ Spent Grain Conversion into Lactic Acid

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Rossana Liguori ◽  
Carlos Ricardo Soccol ◽  
Luciana Porto de Souza Vandenberghe ◽  
Adenise Lorenci Woiciechowski ◽  
Elena Ionata ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Synthetic Medium ◽  
Glucose Consumption ◽  
Acid Yield ◽  
Aqueous Ammonia Soaking ◽  
Spent Grain ◽  
Selection Of

SixLactobacillusstrains were analyzed to select a bacterium for conversion of brewers’ spent grain (BSG) into lactic acid. Among the investigated strains,L. acidophilusATCC 43121 showed the highest yield of lactic acid production (16.1 g/L after 48 hours) when grown in a synthetic medium. It was then analyzed for its ability to grow on the hydrolysates obtained from BSG after acid-alkaline (AAT) or aqueous ammonia soaking (AAS) pretreatment. The lactic acid production byL. acidophilusATCC 43121 through fermentation of the hydrolysate from AAS treated BSG was 96% higher than that from the AAT treated one, although similar yields of lactic acid per consumed glucose were achieved due to a higher (46%) glucose consumption byL. acidophilusATCC 43121 in the AAS BSG hydrolysate. It is worth noting that adding yeast extract to the BSG hydrolysates increased both the yield of lactic acid per substrate consumed and the volumetric productivity. The best results were obtained by fermentation of AAS BSG hydrolysate supplemented by yeast extract, in which the strain produced 22.16 g/L of lactic acid (yield of 0.61 g/g), 27% higher than the value (17.49 g/L) obtained in the absence of a nitrogen source.

scholarly journals Evaluation of biological production of lactic acid in a synthetic medium and in Aloe vera (L.) Burm. f. processing by-products

2015 ◽  
Vol 20 (3) ◽  
pp. 369 ◽  
Author(s):  
Javier Antonio Gómez-Gómez ◽  
Catalina Giraldo-Estrada ◽  
David Habeych ◽  
Sandra Baena
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Synthetic Medium ◽  
Aloe Vera ◽  
Acid Yield ◽  
Growing Conditions ◽  
Defined Culture ◽  
By Products

This study evaluated lactic acid production through batch fermentation in a bioreactor with <em>Thermoanaerobacter</em> sp. strain USBA-018 and a chemically defined culture medium and with hydrolyzed pressed extract of <em>Aloe vera</em> peel (AHE). The strain USBA-018 fermented various sugars, but its primary end-product was L-lactic acid. Factors which influenced L- lactic acid production were pH, addition of yeast extract (YE) and manganese chloride. Under the most favorable growing conditions for the production of lactic acid, yield (Yp/s) increased from 0.66 to 0.96 g/g with a productivity (Qp) of 0.62 g.l-1.h and a maximum lactic acid concentration of 178 mM at 26 hours of fermentation. When AHE was used, 93.3 mM, or 0.175 g.h/L, was obtained. These results show the potential for transformation of sugars that strain USBA-018 offers, but additional studies are needed to find out if different strategies using AHE as carbon source can produce large enough quantities of lactic acid to allow AHE to become a low-cost alternative substrate.

scholarly journals GLUCOSE CONSUMPTION AND LACTIC ACID PRODUCTION BY ERYTHROCYTES FROM DOUBLE-MUSCLED CATTLE

1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON
Keyword(s):  
Lactic Acid ◽  
Key Words ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Age Groups ◽  
Phenotypic Expression ◽  
Glucose Consumption ◽  
Molar Ratio ◽  
Breed Group

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid

Carbohydrate Metabolism of Chick Fibroblasts in Vitro

1942 ◽  
Vol 18 (3) ◽  
pp. 237-256
Author(s):  
E. N. WILLMER
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Acid Production ◽  
Lactic Acid Production ◽  
High Growth ◽  
Glucose Consumption ◽  
High Growth Rate ◽  
Absolute Correlation ◽  
Heart Cultures

1. The glucose consumption of osteoblasts growing in a medium of plasma and embryo juice has been compared with that of the same cells in plasma and Tyrode medium. Embryo juice causes an initial increased glucose consumption which later falls below the level characteristic of a Tyrode-plasma medium. 2. A modification of a method of measuring the nucleoprotein phosphorus content of tissue cultures is described and among other data obtained the changes which occur in this figure have been noted for fourth passage chick osteoblast cultures and for fresh heart explants during the first 4 days of culture in media of plasma and Tyrode and of plasma and embryo juice. 3. The figures for nucleoprotein content have been correlated with those for glucose consumption and lactic acid production and pronounced differences have been observed in the behaviour of the two types of tissue. The possible significance of the findings is discussed. 4. There is no absolute correlation between high growth rate and high sugar consumption or lactic acid production. 5. The data discussed are consistent with the idea that an increased protein metabolism, and a decreased carbohydrate metabolism result from the addition of embryo juice to osteoblast cultures. Embryo juice has no detectable effects on the carbohydrate metabolism of fresh heart cultures.

scholarly journals Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 146-153 ◽  
Author(s):  
Gharwalová Lucia ◽  
Paulová Leona ◽  
Patáková Petra ◽  
Branská Barbora ◽  
Melzoch Karel
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Nitrogen Sources ◽  
Batch Process ◽  
Complete Medium ◽  
Biotechnological Production ◽  
Acid Yield

Biotechnological production of lactic acid has experienced a boom that is hindered only by the lack of low-cost, abundant material that might be used as a substrate for lactic acid bacteria. Such material should contain not only carbon but also complex nitrogen sources, amino acids and vitamins necessary for the balanced growth of the bacteria. Here, for the first time, a combination of hydrolysates of wheat straw and chicken feathers was used as a complete waste cultivation medium for lactic acid production. It was shown to be a promising substrate for lactic acid production, reducing the medium price by 73% compared with MRS broth, providing more than 98% lactic acid yield and high productivity (2.28 ± 0.68 g/l/h) in a fed-batch process using Lactobacillus reuterii LHR14.

scholarly journals The Influence of by-Products From Brewery Spent Grain Hydrolysis on Lactic Acid Fermentation Efficiency

2021 ◽  
Author(s):  
Magdalena Lech
Keyword(s):  
Lactic Acid ◽  
Production Efficiency ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Glucose Consumption ◽  
Raw Material ◽  
Acid Fermentation ◽  
Spent Grain ◽  
Fermentable Carbohydrates ◽  
By Products

Abstract The brewery spent grain (BSG) is a lignocellulosic waste material produced in a huge amount around the world. Strict environmental protection law requires proper utilization. BSG can be transformed into easy-fermentable carbohydrates as a result of hydrolysis. This may be a low-cost raw material for biotechnological fermentation. The literature provides a lot of information that hydrolysis of lignocellulose creates by-products which can be potentially noxious to bacteria cells employed in fermentation. This research examined the influence of most of these by-products: furfural, acetic, formic, gallic, and levuilnic acid, on the LA fermentation effectiveness. These components were introduced to Lactobacillus cultures in various concentrations. The rate of cell growth, glucose consumption, and lactic acid production were measured. This components affects in various extent on LA formation in the culture. In any case, there is a critical value of them harms the fermentation, due to the reduced ability of bacteria propagation. Lower concentrations of bio-catalyst lead to an LA production efficiency drop. The LA concentrations in flasks after one day of propagation with the 2.1 [g/L] of appropriate inhibitor drop app. [%] 16 (F), 22 (GA), 8 (LevA), 40 (AA), and 100 (FA) in comparison to the flask without any inhibitor.

scholarly journals Impact of Hydrolysis Methods on the Utilization of Agricultural Residues as Nutrient Source for D-lactic Acid Production by Sporolactobacillus inulinus

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 12 ◽  
Author(s):  
Silvia Brock ◽  
Anja Kuenz ◽  
Ulf Prüße
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Industrial Process ◽  
Rapeseed Meal ◽  
Agricultural Residues ◽  
Nutrient Source ◽  
Nutrient Sources ◽  
Cheap Alternative

d-lactic acid is a building block for heat resistant polylactic acid, a biobased polymer with a high potential. Nevertheless, an economically efficient industrial process for d-lactic acid production still needs to be implemented. Yeast extract is an expensive nutrient source, which is used to fulfill the complex nutritional requirements in lactic acid fermentations. The substitution of yeast extract by cheap alternative nutrient sources is a challenge in many fermentation processes. In this study, chemical and enzymatic hydrolysis techniques for protein rich agricultural residues and their effectiveness are compared, as well as their impact on the d-lactic acid production of Sporolactobacillus inulinus. An efficient substitution of yeast extract could be achieved by a variety of agricultural residues, hydrolysed with 3M H2SO4, demonstrating the much higher versatility and effectiveness of this method compared to enzymatic methods. In a fed-batch experiment with chemically hydrolyzed rapeseed meal and minimal supplementation, a lactic acid titer of 221 g L−1 and an overall productivity of 1.55 g (L h)−1 (96% yield) were obtained.

Nutritional requirements and the impact of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus

2017 ◽  
Vol 19 (19) ◽  
pp. 4633-4641 ◽  
Author(s):  
Silvia Klotz ◽  
Anja Kuenz ◽  
Ulf Prüße
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Nutritional Requirements ◽  
The Impact ◽  
First Time

For the first time, nutritional requirements including the effects of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus are presented.

Effects of medium supplementation and pH control on lactic acid production from brewer's spent grain

2008 ◽  
Vol 40 (3) ◽  
pp. 437-444 ◽  
Author(s):  
Solange I. Mussatto ◽  
Marcela Fernandes ◽  
Ismael M. Mancilha ◽  
Inês C. Roberto
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Ph Control ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Medium Supplementation
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