scholarly journals Hydrolyzed Agricultural Residues—Low-Cost Nutrient Sources for l-Lactic Acid Production

Fermentation ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 97 ◽  
Author(s):  
Susan Krull ◽  
Silvia Brock ◽  
Ulf Prüße ◽  
Anja Kuenz
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Rapeseed Meal ◽  
Agricultural Residues ◽  
Nutrient Sources ◽  
Protein Rich

Lactic acid is a building block for polylactic acid, which is one of the most promising polymers based on renewable resources and is used mainly in packaging industry. This bio-based polymer is biodegradable and provides an ecological and economical alternative to petrochemical plastics. The largest cost blocks of biotechnological lactic acid production, accounting for up to 38% of the total costs, are substrate and nutrient sources, such as peptone, meat, and yeast extract. Based on a systematic analysis of nutritional requirements, the substitution of yeast extract by low-cost protein-rich agricultural hydrolysates was estimated for the production of l-lactic acid with Lactobacillus casei. Cultivations in 24-well microtiter plates enabled analysis of nutrient requirements and the usage of various hydrolysates with a high parallel throughput and repeated sampling. Rapeseed meal (RM) and distillers’ dried grains with solubles (DDGS) were tested as low-cost protein-rich agricultural residues. By using chemically or enzymatically hydrolyzed rapeseed meal or DDGS, 70% of the nutrient sources was replaced in the fermentation process at identical productivity and product yields. All in all, the total costs of l-lactic acid production with Lactobacillus casei could potentially be reduced by up to 23%.

scholarly journals Impact of Hydrolysis Methods on the Utilization of Agricultural Residues as Nutrient Source for D-lactic Acid Production by Sporolactobacillus inulinus

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 12 ◽  
Author(s):  
Silvia Brock ◽  
Anja Kuenz ◽  
Ulf Prüße
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Industrial Process ◽  
Rapeseed Meal ◽  
Agricultural Residues ◽  
Nutrient Source ◽  
Nutrient Sources ◽  
Cheap Alternative

d-lactic acid is a building block for heat resistant polylactic acid, a biobased polymer with a high potential. Nevertheless, an economically efficient industrial process for d-lactic acid production still needs to be implemented. Yeast extract is an expensive nutrient source, which is used to fulfill the complex nutritional requirements in lactic acid fermentations. The substitution of yeast extract by cheap alternative nutrient sources is a challenge in many fermentation processes. In this study, chemical and enzymatic hydrolysis techniques for protein rich agricultural residues and their effectiveness are compared, as well as their impact on the d-lactic acid production of Sporolactobacillus inulinus. An efficient substitution of yeast extract could be achieved by a variety of agricultural residues, hydrolysed with 3M H2SO4, demonstrating the much higher versatility and effectiveness of this method compared to enzymatic methods. In a fed-batch experiment with chemically hydrolyzed rapeseed meal and minimal supplementation, a lactic acid titer of 221 g L−1 and an overall productivity of 1.55 g (L h)−1 (96% yield) were obtained.

scholarly journals Statistical Optimization of Lactic Acid Production by Lactobacillus pentosus using Hemicellulosic Hydrolysate from Sugarcane Bagasse

2018 ◽  
Vol 29 (1) ◽  
pp. 41-51
Author(s):  
Daiana Wischral
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Hemicellulose Hydrolysate ◽  
Anaerobic Conditions ◽  
Lactobacillus Pentosus

Lactic acid, traditionally obtained through fermentation processes, presents numerous applications in the chemical industry. Among these is the production of polymers, more specifically biodegradable polylactic acid (PLA). Development of processes that use low cost substrates, such as bioproduction of lactic acid, could improve the economic viability of bioprocesses. Thus, the present work reports investigation of hemicellulose hydrolysate from sugarcane bagasse as a sole carbon source for lactic acid production by Lactobacillus pentosus ATCC 8041. Initially, sugarcane bagasse was pretreated with acid in a solid:liquid ratio of 1:2.8 (1 g of bagasse: 2.8 mL of sulfuric acid solution 1 % v/v) and at a temperature of 121°C for 27 minutes. Then, concentration of both the hemicellulose hydrolysate and the yeast extract in MRS medium were optimized usingResponse Surface Methodology through software STATISTICA 6.0. Once the optimal conditions (40 % of hemicellulose hydrolysate and 5 g/L of yeast extract) were validated, fermentations were carried out in anaerobic conditions at 37°C and 120 rpm. After 48h, 19.17 g/L of lactic acid were produced, corresponding to a volumetric productivity of 0.40 g/L.h1. Findings of this work demonstrate that hemicellulose hydrolysate from sugarcane bagasse is a promising carbon source for lactic acid production.

L(+)-Lactic Acid Production Using Lactobacillus Casei in Solid-State Fermentation

2005 ◽  
Vol 27 (21) ◽  
pp. 1685-1688 ◽  
Author(s):  
Pappy John Rojan ◽  
Kesavan Madhavan Nampoothiri ◽  
Athira Syamaprasad Nair ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production

scholarly journals Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 146-153 ◽  
Author(s):  
Gharwalová Lucia ◽  
Paulová Leona ◽  
Patáková Petra ◽  
Branská Barbora ◽  
Melzoch Karel
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Nitrogen Sources ◽  
Batch Process ◽  
Complete Medium ◽  
Biotechnological Production ◽  
Acid Yield

Biotechnological production of lactic acid has experienced a boom that is hindered only by the lack of low-cost, abundant material that might be used as a substrate for lactic acid bacteria. Such material should contain not only carbon but also complex nitrogen sources, amino acids and vitamins necessary for the balanced growth of the bacteria. Here, for the first time, a combination of hydrolysates of wheat straw and chicken feathers was used as a complete waste cultivation medium for lactic acid production. It was shown to be a promising substrate for lactic acid production, reducing the medium price by 73% compared with MRS broth, providing more than 98% lactic acid yield and high productivity (2.28 ± 0.68 g/l/h) in a fed-batch process using Lactobacillus reuterii LHR14.

Lactic acid production from whey permeate by immobilized Lactobacillus casei

1985 ◽  
Vol 7 (4) ◽  
pp. 164-168 ◽  
Author(s):  
Amita Tuli ◽  
R.P. Sethi ◽  
P.K. Khanna ◽  
S.S. Marwaha ◽  
J.F. Kennedy
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Whey Permeate
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