Thermostable Branched DNA Nanostructures as Modular Primers for Polymerase Chain Reaction

2013 ◽  
Vol 52 (33) ◽  
pp. 8699-8702 ◽  
Author(s):  
Mark R. Hartman ◽  
Dayong Yang ◽  
Thua N. N. Tran ◽  
Kwang Lee ◽  
Jason S. Kahn ◽  
...  
2013 ◽  
Vol 125 (33) ◽  
pp. 8861-8864 ◽  
Author(s):  
Mark R. Hartman ◽  
Dayong Yang ◽  
Thua N. N. Tran ◽  
Kwang Lee ◽  
Jason S. Kahn ◽  
...  

2008 ◽  
Vol 80 (24) ◽  
pp. 9402-9410 ◽  
Author(s):  
Ai-Cheng Lee ◽  
Ziyu Dai ◽  
Baowei Chen ◽  
Hong Wu ◽  
Jun Wang ◽  
...  

Nano Letters ◽  
2009 ◽  
Vol 9 (12) ◽  
pp. 4302-4305 ◽  
Author(s):  
Elisabeth Pound ◽  
Jeffrey R. Ashton ◽  
Héctor A. Becerril ◽  
Adam T. Woolley

2000 ◽  
Vol 20 (3) ◽  
pp. 257-261 ◽  
Author(s):  
Emmanuel Debono ◽  
Philippe Halfon ◽  
Marc Bourliere ◽  
Victoria Gerolami-Santandrea ◽  
Marguerite Gastaldi ◽  
...  

Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.


2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske

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