Super-resolution imaging of self-assembly of amphiphilic photoswitchable macrocycles

2017 ◽  
Vol 53 (18) ◽  
pp. 2669-2672 ◽  
Author(s):  
Qiong-Xin Hua ◽  
Bo Xin ◽  
Zu-Jing Xiong ◽  
Wen-Liang Gong ◽  
Chong Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Super Resolution ◽  
Fluorescence Microscope ◽  
Optical Diffraction ◽  
Tetraethylene Glycol ◽  
Resolution Imaging ◽  
Diffraction Limitation

Self-assembly of an amphiphilic photoswitchable fluorescent macrocycle methoxy-tetraethylene glycol-substituted hexaarylbiimidazole-borondipyrromethene can be observed directly under a super-resolution fluorescence microscope, with the nanoscale resolution beyond the optical diffraction limitation.

scholarly journals Large-Scale Fabrication of Photonic Nanojet Array via Template-Assisted Self-Assembly

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 473 ◽  
Author(s):  
Pengcheng Zhang ◽  
Xi Chen ◽  
Hui Yang
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Super Resolution ◽  
Light Signal ◽  
Practical Applications ◽  
Photonic Nanojet ◽  
Detection Technology ◽  
Size Uniformity ◽  
Resolution Imaging ◽  
Photonic Nanojets

A large-scale homogenized photonic nanojet array with defined pattern and spacing facilitates practical applications in super-resolution imaging, subwavelength-resolution nanopatterning, nano objects trapping and detection technology. In this paper, we present the fabrication of a large-scale photonic nanojet array via the template-assisted self-assembly (TASA) approach. Templates of two-dimensional (2D) large-scale microwell array with defined pattern and spacing are fabricated. Melamine microspheres with excellent size uniformity are utilized to pattern on the template. It is found that microwells can be filled at a yield up to 95%. These arrayed microspheres on the template serve as microlenses and can be excited to generate large-scale photonic nanojets. The uniformly-sized melamine spheres are beneficial for the generation of a homogenized photonic nanojet array. The intensity of the photonic nanojets in water is as high as ~2 fold the background light signal. Our work shows a simple, robust, and fast means for the fabrication of a large-scale homogenized photonic nanojet array.

scholarly journals Recent Progress in the Correlative Structured Illumination Microscopy

Chemosensors ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 364
Author(s):  
Meiting Wang ◽  
Jiajie Chen ◽  
Lei Wang ◽  
Xiaomin Zheng ◽  
Jie Zhou ◽  
...  
Keyword(s):  
High Frequency ◽  
Optical Transmission ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Recent Developments ◽  
Resolution Imaging

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

scholarly journals Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jason C. Sang ◽  
Eric Hidari ◽  
Georg Meisl ◽  
Rohan T. Ranasinghe ◽  
Maria Grazia Spillantini ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Self Assembly ◽  
Super Resolution ◽  
Quantitative Information ◽  
Protein Homeostasis ◽  
Protective Response ◽  
Resolution Imaging ◽  
Key Steps ◽  
Super Resolution Microscopy ◽  
The Brain

AbstractAggregation of α-synuclein (α-syn) is closely linked to Parkinson’s disease (PD) and the related synucleinopathies. Aggregates spread through the brain during the progression of PD, but the mechanism by which this occurs is still not known. One possibility is a self-propagating, templated-seeding mechanism, but this cannot be established without quantitative information about the efficiencies and rates of the key steps in the cellular process. To address this issue, we imaged the uptake and seeding of unlabeled exogenous α-syn fibrils by SH-SY5Y cells and the resulting secreted aggregates, using super-resolution microscopy. Externally-applied fibrils very inefficiently induced self-assembly of endogenous α-syn in a process accelerated by the proteasome. Seeding resulted in the increased secretion of nanoscopic aggregates (mean 35 nm diameter), of both α-syn and Aβ. Our results suggest that cells respond to seed-induced disruption of protein homeostasis predominantly by secreting nanoscopic aggregates; this mechanism may therefore be an important protective response by cells to protein aggregation.

scholarly journals Multilevel phase supercritical lens fabricated by synergistic optical lithography

Nanophotonics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1469-1477 ◽  
Author(s):  
Wei Fang ◽  
Jian Lei ◽  
Pengda Zhang ◽  
Fei Qin ◽  
Meiling Jiang ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Energy Concentration ◽  
Focal Region ◽  
Movement Trajectory ◽  
Label Free ◽  
Laser Fabrication ◽  
Resolution Imaging

AbstractThe advent of planar metalenses, including the super-oscillatory lens (SOL) and the supercritical lens (SCL) with distinctive interference properties, has profoundly impacted on the long-lasting perception of the far-field optical diffraction limit. In spite of its conspicuous success in achieving marvelously small focal spots, the planar metalens still faces tough design and fabrication challenges to realize high focusing efficiency. In this work, we demonstrated a dual-mode laser fabrication technique based on two-photon polymerization for realizing the multilevel phase SCL with focusing efficiency spiking. Synergistically controlling two types of movement trajectory, which is implemented with the piezo stage and the scanning galvo mirror, enables the fabrication of complicated structures with sub-diffraction-limit feature size. By utilizing such advantage, SCLs with discretized multilevel phase configurations are explicitly patterned. The experimental characterization results have shown that a four-level phase SCL can focus light into a sub-diffraction-limit spot with the lateral size of 0.41 λ/NA (NA is the numerical aperture), while achieve the focal spot intensity and the energy concentration ratio in the focal region 7.2 times and 3 times that of the traditional binary amplitude-type SCL with the same optimization conditions, respectively. Our results may release the application obstacles for the sub-diffraction-limit planar metalens and enable major advances in the fields from label-free optical super-resolution imaging to high precision laser fabrication.

scholarly journals Nanoscopy on the Chea(i)p

2020 ◽  
Author(s):  
Benedict Diederich ◽  
Øystein Helle ◽  
Patrick Then ◽  
Pablo Carravilla ◽  
Kay Oliver Schink ◽  
...  
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Low Cost ◽  
Super Resolution ◽  
Image Sensor ◽  
Optical Diffraction ◽  
Sensor Technology ◽  
Safety Level ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

AbstractSuper-resolution microscopy allows for stunning images with a resolution well beyond the optical diffraction limit, but the imaging techniques are demanding in terms of instrumentation and software. Using scientific-grade cameras, solid-state lasers and top-shelf microscopy objective lenses drives the price and complexity of the system, limiting its use to well-funded institutions. However, by harnessing recent developments in CMOS image sensor technology and low-cost illumination strategies, super-resolution microscopy can be made available to the mass-markets for a fraction of the price. Here, we present a 3D printed, self-contained super-resolution microscope with a price tag below 1000 $ including the objective and a cellphone. The system relies on a cellphone to both acquire and process images as well as control the hardware, and a photonic-chip enabled illumination. The system exhibits 100nm optical resolution using single-molecule localization microscopy and can provide live super-resolution imaging using light intensity fluctuation methods. Furthermore, due to its compactness, we demonstrate its potential use inside bench-top incubators and high biological safety level environments imaging SARS-CoV-2 viroids. By the development of low-cost instrumentation and by sharing the designs and manuals, the stage for democratizing super-resolution imaging is set.

scholarly journals Super-Resolution Imaging and Quantitative Analysis of Membrane Protein/Lipid Raft Clustering Mediated by Cell-Surface Self-Assembly of Hybrid Nanoconjugates

ChemBioChem ◽  
2015 ◽  
Vol 16 (12) ◽  
pp. 1725-1729 ◽  
Author(s):  
Jonathan M. Hartley ◽  
Te-Wei Chu ◽  
Eric M. Peterson ◽  
Rui Zhang ◽  
Jiyuan Yang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Membrane Protein ◽  
Cell Surface ◽  
Lipid Raft ◽  
Self Assembly ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Raft Clustering

A Polymerizable Photoswitchable Fluorophore for Super-Resolution Imaging of Polymer Self-Assembly and Dynamics

2018 ◽  
Vol 7 (12) ◽  
pp. 1432-1437 ◽  
Author(s):  
Zhe Qiang ◽  
Kevin M. Shebek ◽  
Masahiro Irie ◽  
Muzhou Wang
Keyword(s):  
Self Assembly ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Metrology of DNA arrays by super-resolution microscopy

Nanoscale ◽  
2017 ◽  
Vol 9 (29) ◽  
pp. 10205-10211 ◽  
Author(s):  
Christopher M. Green ◽  
Kelly Schutt ◽  
Noah Morris ◽  
Reza M. Zadegan ◽  
William L. Hughes ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Self Assembly ◽  
Super Resolution ◽  
Dna Nanostructures ◽  
Dna Arrays ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

Crystal-PAINT super-resolution imaging enables high-throughput metrology of DNA nanostructures for quantitative analysis of arrays formed through self-assembly.

Programmable in vitro Co-Encapsidation of Guest Proteins Inside Protein Nanocages

2018 ◽  
Author(s):  
Noor H. Dashti ◽  
Rufika S. Abidin ◽  
Frank Sainsbury
Keyword(s):  
Self Assembly ◽  
Mammalian Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Cell Engineering ◽  
Particle Analysis ◽  
Protein Cages

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both <i>in vitro</i> and <i>in vivo</i> cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for <i>in vivo</i> self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by <i>in vitro</i> self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

Probing the Dynamic Self-Assembly Behaviour of Photoswitchable Wormlike Micelles in Real Time

2018 ◽  
Author(s):  
Elaine A. Kelly ◽  
Judith E. Houston ◽  
Rachel Evans
Keyword(s):  
Real Time ◽  
Absorption Spectroscopy ◽  
Self Assembly ◽  
Uv Light ◽  
Wormlike Micelles ◽  
Tetraethylene Glycol ◽  
Light Illumination ◽  
First Time ◽  
Dependent Processes

Understanding the dynamic self-assembly behaviour of azobenzene photosurfactants (AzoPS) is crucial to advance their use in controlled release applications such as<i></i>drug delivery and micellar catalysis. Currently, their behaviour in the equilibrium <i>cis-</i>and <i>trans</i>-photostationary states is more widely understood than during the photoisomerisation process itself. Here, we investigate the time-dependent self-assembly of the different photoisomers of a model neutral AzoPS, <a>tetraethylene glycol mono(4′,4-octyloxy,octyl-azobenzene) </a>(C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>) using small-angle neutron scattering (SANS). We show that the incorporation of <i>in-situ</i>UV-Vis absorption spectroscopy with SANS allows the scattering profile, and hence micelle shape, to be correlated with the extent of photoisomerisation in real-time. It was observed that C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>could switch between wormlike micelles (<i>trans</i>native state) and fractal aggregates (under UV light), with changes in the self-assembled structure arising concurrently with changes in the absorption spectrum. Wormlike micelles could be recovered within 60 seconds of blue light illumination. To the best of our knowledge, this is the first time the degree of AzoPS photoisomerisation has been tracked <i>in</i><i>-situ</i>through combined UV-Vis absorption spectroscopy-SANS measurements. This technique could be widely used to gain mechanistic and kinetic insights into light-dependent processes that are reliant on self-assembly.

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