scholarly journals Metrology of DNA arrays by super-resolution microscopy

Nanoscale ◽  
2017 ◽  
Vol 9 (29) ◽  
pp. 10205-10211 ◽  
Author(s):  
Christopher M. Green ◽  
Kelly Schutt ◽  
Noah Morris ◽  
Reza M. Zadegan ◽  
William L. Hughes ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Self Assembly ◽  
Super Resolution ◽  
Dna Nanostructures ◽  
Dna Arrays ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

Crystal-PAINT super-resolution imaging enables high-throughput metrology of DNA nanostructures for quantitative analysis of arrays formed through self-assembly.

scholarly journals Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jason C. Sang ◽  
Eric Hidari ◽  
Georg Meisl ◽  
Rohan T. Ranasinghe ◽  
Maria Grazia Spillantini ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Self Assembly ◽  
Super Resolution ◽  
Quantitative Information ◽  
Protein Homeostasis ◽  
Protective Response ◽  
Resolution Imaging ◽  
Key Steps ◽  
Super Resolution Microscopy ◽  
The Brain

AbstractAggregation of α-synuclein (α-syn) is closely linked to Parkinson’s disease (PD) and the related synucleinopathies. Aggregates spread through the brain during the progression of PD, but the mechanism by which this occurs is still not known. One possibility is a self-propagating, templated-seeding mechanism, but this cannot be established without quantitative information about the efficiencies and rates of the key steps in the cellular process. To address this issue, we imaged the uptake and seeding of unlabeled exogenous α-syn fibrils by SH-SY5Y cells and the resulting secreted aggregates, using super-resolution microscopy. Externally-applied fibrils very inefficiently induced self-assembly of endogenous α-syn in a process accelerated by the proteasome. Seeding resulted in the increased secretion of nanoscopic aggregates (mean 35 nm diameter), of both α-syn and Aβ. Our results suggest that cells respond to seed-induced disruption of protein homeostasis predominantly by secreting nanoscopic aggregates; this mechanism may therefore be an important protective response by cells to protein aggregation.

scholarly journals Super-Resolution Imaging and Quantitative Analysis of Membrane Protein/Lipid Raft Clustering Mediated by Cell-Surface Self-Assembly of Hybrid Nanoconjugates

ChemBioChem ◽  
2015 ◽  
Vol 16 (12) ◽  
pp. 1725-1729 ◽  
Author(s):  
Jonathan M. Hartley ◽  
Te-Wei Chu ◽  
Eric M. Peterson ◽  
Rui Zhang ◽  
Jiyuan Yang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Membrane Protein ◽  
Cell Surface ◽  
Lipid Raft ◽  
Self Assembly ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Raft Clustering

scholarly journals Metrology of DNA arrays by super-resolution microscopy

2017 ◽  
Author(s):  
Christopher Green ◽  
Kelly Schutt ◽  
Noah Morris ◽  
William L. Hughes ◽  
Wan Kuang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Dna Arrays ◽  
Super Resolution Microscopy

scholarly journals Large-Scale Fabrication of Photonic Nanojet Array via Template-Assisted Self-Assembly

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 473 ◽  
Author(s):  
Pengcheng Zhang ◽  
Xi Chen ◽  
Hui Yang
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Super Resolution ◽  
Light Signal ◽  
Practical Applications ◽  
Photonic Nanojet ◽  
Detection Technology ◽  
Size Uniformity ◽  
Resolution Imaging ◽  
Photonic Nanojets

A large-scale homogenized photonic nanojet array with defined pattern and spacing facilitates practical applications in super-resolution imaging, subwavelength-resolution nanopatterning, nano objects trapping and detection technology. In this paper, we present the fabrication of a large-scale photonic nanojet array via the template-assisted self-assembly (TASA) approach. Templates of two-dimensional (2D) large-scale microwell array with defined pattern and spacing are fabricated. Melamine microspheres with excellent size uniformity are utilized to pattern on the template. It is found that microwells can be filled at a yield up to 95%. These arrayed microspheres on the template serve as microlenses and can be excited to generate large-scale photonic nanojets. The uniformly-sized melamine spheres are beneficial for the generation of a homogenized photonic nanojet array. The intensity of the photonic nanojets in water is as high as ~2 fold the background light signal. Our work shows a simple, robust, and fast means for the fabrication of a large-scale homogenized photonic nanojet array.

scholarly journals Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells

Nanoscale ◽  
2019 ◽  
Vol 11 (20) ◽  
pp. 10023-10033 ◽  
Author(s):  
Jan Bergstrand ◽  
Lei Xu ◽  
Xinyan Miao ◽  
Nailin Li ◽  
Ozan Öktem ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Dictionary Learning ◽  
Super Resolution ◽  
Distribution Patterns ◽  
Specific Protein ◽  
Protein Distribution ◽  
Activated Platelets ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

Super-resolution imaging of P-selectin in platelets together with dictionary learning allow specifically activated platelets to be identified in an automatic objective manner.

scholarly journals 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples

Methods ◽  
2020 ◽  
Vol 174 ◽  
pp. 56-71 ◽  
Author(s):  
Ruisheng Lin ◽  
Alexander H. Clowsley ◽  
Tobias Lutz ◽  
David Baddeley ◽  
Christian Soeller
Keyword(s):  
Quantitative Analysis ◽  
Super Resolution ◽  
Dna Origami ◽  
Super Resolution Microscopy
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