scholarly journals Super-Resolution Imaging and Quantitative Analysis of Membrane Protein/Lipid Raft Clustering Mediated by Cell-Surface Self-Assembly of Hybrid Nanoconjugates

ChemBioChem ◽  
2015 ◽  
Vol 16 (12) ◽  
pp. 1725-1729 ◽  
Author(s):  
Jonathan M. Hartley ◽  
Te-Wei Chu ◽  
Eric M. Peterson ◽  
Rui Zhang ◽  
Jiyuan Yang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Membrane Protein ◽  
Cell Surface ◽  
Lipid Raft ◽  
Self Assembly ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Raft Clustering

scholarly journals Metrology of DNA arrays by super-resolution microscopy

Nanoscale ◽  
2017 ◽  
Vol 9 (29) ◽  
pp. 10205-10211 ◽  
Author(s):  
Christopher M. Green ◽  
Kelly Schutt ◽  
Noah Morris ◽  
Reza M. Zadegan ◽  
William L. Hughes ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Self Assembly ◽  
Super Resolution ◽  
Dna Nanostructures ◽  
Dna Arrays ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

Crystal-PAINT super-resolution imaging enables high-throughput metrology of DNA nanostructures for quantitative analysis of arrays formed through self-assembly.

Exploring cell surface-nanopillar interactions with 3D super-resolution imaging

2021 ◽  
Author(s):  
Anish R. Roy ◽  
Wei Zhang ◽  
Zeinab Jahed ◽  
Ching-Ting Tsai ◽  
Bianxiao Cui ◽  
...  
Keyword(s):  
Cell Surface ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Large-Scale Fabrication of Photonic Nanojet Array via Template-Assisted Self-Assembly

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 473 ◽  
Author(s):  
Pengcheng Zhang ◽  
Xi Chen ◽  
Hui Yang
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Super Resolution ◽  
Light Signal ◽  
Practical Applications ◽  
Photonic Nanojet ◽  
Detection Technology ◽  
Size Uniformity ◽  
Resolution Imaging ◽  
Photonic Nanojets

A large-scale homogenized photonic nanojet array with defined pattern and spacing facilitates practical applications in super-resolution imaging, subwavelength-resolution nanopatterning, nano objects trapping and detection technology. In this paper, we present the fabrication of a large-scale photonic nanojet array via the template-assisted self-assembly (TASA) approach. Templates of two-dimensional (2D) large-scale microwell array with defined pattern and spacing are fabricated. Melamine microspheres with excellent size uniformity are utilized to pattern on the template. It is found that microwells can be filled at a yield up to 95%. These arrayed microspheres on the template serve as microlenses and can be excited to generate large-scale photonic nanojets. The uniformly-sized melamine spheres are beneficial for the generation of a homogenized photonic nanojet array. The intensity of the photonic nanojets in water is as high as ~2 fold the background light signal. Our work shows a simple, robust, and fast means for the fabrication of a large-scale homogenized photonic nanojet array.

Super-resolution imaging of self-assembly of amphiphilic photoswitchable macrocycles

2017 ◽  
Vol 53 (18) ◽  
pp. 2669-2672 ◽  
Author(s):  
Qiong-Xin Hua ◽  
Bo Xin ◽  
Zu-Jing Xiong ◽  
Wen-Liang Gong ◽  
Chong Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Super Resolution ◽  
Fluorescence Microscope ◽  
Optical Diffraction ◽  
Tetraethylene Glycol ◽  
Resolution Imaging ◽  
Diffraction Limitation

Self-assembly of an amphiphilic photoswitchable fluorescent macrocycle methoxy-tetraethylene glycol-substituted hexaarylbiimidazole-borondipyrromethene can be observed directly under a super-resolution fluorescence microscope, with the nanoscale resolution beyond the optical diffraction limitation.

scholarly journals A DNA nanoassembly-based approach to map membrane protein nanoenvironments

2019 ◽  
Author(s):  
Elena Ambrosetti ◽  
Giulio Bernardinelli ◽  
Ian Hoffecker ◽  
Leonard Hartmanis ◽  
Rickard Sandberg ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Function ◽  
Spatial Organization ◽  
Super Resolution ◽  
Resolution Method ◽  
Resolution Imaging ◽  
Functional Relevance ◽  
Ensemble Analysis ◽  
Membrane Protein Function ◽  
Comprehensive Mapping

AbstractSuper-resolution imaging has revealed that most proteins at the plasma membrane are not uniformly distributed but localize to dynamic domains of nanoscale dimensions. To investigate their functional relevance, there is a need for methods that enable comprehensive mapping of the compositions and spatial organizations of membrane protein nanodomains in cell populations. However, current superresolution methods are limited to analysing small, preselected subsets of proteins, at very low sampling fractions. Here we describe the development of a non-microscopy based super-resolution method for unbiased ensemble analysis of membrane protein nanodomains. The method, termed NANOscale DEciphEring of membrane Protein nanodomains (NanoDeep), is based on the use of DNA nanoassemblies to translate membrane protein organization information into a DNA sequencing readout. Using NanoDeep, we characterized the nanoenvironments of Her2, a membrane receptor of critical relevance in cancer. We found that the occupancies of Her2, Her3 and EGFR in the nanoenvironments surrounding Her2 were similar in two cell lines with vastly different expression levels of Her2. Further, we found that adding Heregulin-β1 to cancer cells led to increased occupancy of Her2 and Her3, and to a lesser extent EGFR, in Her2 nanoenvironments. NanoDeep has the potential to provide new insights into the roles of the composition and spatial organization of protein nanoenvironments in the regulation of membrane protein function.

scholarly journals FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Matthieu Lagardère ◽  
Ingrid Chamma ◽  
Emmanuel Bouilhol ◽  
Macha Nikolski ◽  
Olivier Thoumine
Keyword(s):  
Membrane Protein ◽  
Protein Dynamics ◽  
Single Molecule ◽  
Imaging Techniques ◽  
Simulated Data ◽  
Super Resolution ◽  
Molecular Complexes ◽  
Live Cell ◽  
Resolution Imaging ◽  
Membrane Protein Dynamics

AbstractFluorescence live-cell and super-resolution microscopy methods have considerably advanced our understanding of the dynamics and mesoscale organization of macro-molecular complexes that drive cellular functions. However, different imaging techniques can provide quite disparate information about protein motion and organization, owing to their respective experimental ranges and limitations. To address these issues, we present here a robust computer program, called FluoSim, which is an interactive simulator of membrane protein dynamics for live-cell imaging methods including SPT, FRAP, PAF, and FCS, and super-resolution imaging techniques such as PALM, dSTORM, and uPAINT. FluoSim integrates diffusion coefficients, binding rates, and fluorophore photo-physics to calculate in real time the localization and intensity of thousands of independent molecules in 2D cellular geometries, providing simulated data directly comparable to actual experiments. FluoSim was thoroughly validated against experimental data obtained on the canonical neurexin-neuroligin adhesion complex at cell–cell contacts. This unified software allows one to model and predict membrane protein dynamics and localization at the ensemble and single molecule level, so as to reconcile imaging paradigms and quantitatively characterize protein behavior in complex cellular environments.

scholarly journals Time-Lapse Super-Resolution Imaging of Apical Membrane Protein Domains in Live Filamentous Fungi

2013 ◽  
Vol 104 (2) ◽  
pp. 652a ◽  
Author(s):  
Yuji Ishitsuka ◽  
Yiming Li ◽  
Reinhard Fischer ◽  
Norio Takeshita ◽  
G. Ulrich Nienhaus
Keyword(s):  
Membrane Protein ◽  
Filamentous Fungi ◽  
Apical Membrane ◽  
Protein Domains ◽  
Super Resolution ◽  
Time Lapse ◽  
Resolution Imaging

scholarly journals VMD as a Software for Visualization and Quantitative Analysis of Super Resolution Imaging and Single Particle Tracking

2014 ◽  
Vol 106 (2) ◽  
pp. 202a ◽  
Author(s):  
Yanxin Liu ◽  
John E. Stone ◽  
En Cai ◽  
Jingyi Fei ◽  
Sang Hak Lee ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Resolution Imaging
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