An electrochemiluminescence biosensor for Kras mutations based on locked nucleic acid functionalized DNA walkers and hyperbranched rolling circle amplification

2017 ◽  
Vol 53 (20) ◽  
pp. 2910-2913 ◽  
Author(s):  
Ying Zhang ◽  
Lixu Wang ◽  
Fang Luo ◽  
Bin Qiu ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Locked Nucleic Acid ◽  
Specific Detection ◽  
Kras Mutations ◽  
Rolling Circle ◽  
Dna Walkers ◽  
Mutant Genes

Herein, an electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of Kras mutant genes has been developed.

scholarly journals Highly sensitive and specific screening of EGFR mutation using a PNA microarray-based fluorometric assay based on rolling circle amplification and graphene oxide

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38298-38308 ◽  
Author(s):  
Xiaojun Xu ◽  
Shu Xing ◽  
Mengjia Xu ◽  
Pan Fu ◽  
Tingting Gao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Egfr Mutation ◽  
Rolling Circle Amplification ◽  
Specific Detection ◽  
Fluorometric Method ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Detection Probe

A facile peptide nucleic acid microarray-based fluorometric method was developed for sensitive and specific detection of EGFR mutation by using rolling circle amplification, graphene oxide, and a fluorescently-labeled detection probe.

The analysis of proteins and small molecules based on sterically tunable nucleic acid hyperbranched rolling circle amplification

2017 ◽  
Vol 91 ◽  
pp. 136-142 ◽  
Author(s):  
Hai Shi ◽  
Xiaoxia Mao ◽  
Xiaoxia Chen ◽  
Zihan Wang ◽  
Keming Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Small Molecules ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Mitigating the Non-Specific Amplification in RCA: Assessment of the Role of Ligation and Exonuclease Digestion in Circular DNA Preparation

2021 ◽  
Author(s):  
Vandana Kuttappan Nair ◽  
Chandrika Sharma ◽  
Mrittika Sengupta ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
False Positive ◽  
Rolling Circle Amplification ◽  
Circular Dna ◽  
Rolling Circle ◽  
Specific Amplification ◽  
Exonuclease Digestion ◽  
Sticky End

<b>Layman Summary: </b>Rolling circle amplification (RCA) is a popular and extensively used bioanalytical tool. Like any nucleic acid amplifications, non-specific amplification may occur in it and risk generating false positive readouts. The work described in the manuscript investigates non-specific amplification in RCA as a function of ligation and exonuclease digestion assays during the synthesis of circular DNA. In particular, it investigates and compares the role of three different ligation techniques, namely splint-padlock ligation, cohesive end (sticky end ligation), and self-annealing ligation. In addition, it also probes the role of single exonuclease vs dual exonuclease digestions. We employed real time fluorescence to quantify the effect of these factors. Finally, our work hypothesizes the possible origins of non-specific amplification in RCA.

Screening substrate-binding positions by rolling circle amplification suggesting a binding model of Nt.BstNBI

2019 ◽  
Vol 476 (10) ◽  
pp. 1483-1496
Author(s):  
Hua Wei ◽  
Suming Tang ◽  
Xuying Duan ◽  
Yifu Guan ◽  
Guojie Zhao
Keyword(s):  
Catalytic Mechanism ◽  
Rolling Circle Amplification ◽  
Locked Nucleic Acid ◽  
Dna Duplex ◽  
Recognition Sequence ◽  
Acid Modification ◽  
Binding Model ◽  
Rolling Circle ◽  
Structure Information ◽  
Potential Binding

Abstract Nicking endonucleases (NEs) become increasingly attractive for their promising applications in isothermal amplification. Unfortunately, in comparison with their applications, their catalytic mechanism studies have relatively lagged behind due to a paucity of crystal structure information. Nt.BstNBI is one of those widely used NEs. However, many aspects of its catalytic mechanism still remained to be explored. Herein, we employed only rolling circle amplification (RCA) assay as a major analytic tool and succeeded in identifying the potential binding positions and regions of the DNA substrate based on locked nucleic acid modification, DNA duplex length of substrate, and substrate mismatch designs. Based on these data, we, for the first time, revealed that Nt.BstNBI was likely to recognize six adjacent positions of the recognition sequence (G1rt, A2rt, G3rt, A2rb, C3rb, and T4rb) in the major groove and hold three positions of the cleavage sequence (N3ct, N4ct, and N7cb) in the minor groove of DNA duplex for nicking. Moreover, this work also demonstrated the unexpected efficiency of RCA to study the macromolecular interaction for certain kind of nucleases in an easy and high-throughput way.

Nucleic acid sensing by regenerable surface-associated isothermal rolling circle amplification

2007 ◽  
Vol 22 (7) ◽  
pp. 1236-1244 ◽  
Author(s):  
Erik L. McCarthy ◽  
Lee E. Bickerstaff ◽  
Mauricio Pereira da Cunha ◽  
Paul J. Millard
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Protein binding-protected DNA three-way junction-mediated rolling circle amplification for sensitive and specific detection of transcription factors

RSC Advances ◽  
2016 ◽  
Vol 6 (73) ◽  
pp. 68846-68851 ◽  
Author(s):  
Kan Li ◽  
Lei Wang ◽  
Xiaowen Xu ◽  
Ting Gao ◽  
Ping Yan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Protein Binding ◽  
Rolling Circle Amplification ◽  
Specific Detection ◽  
Rolling Circle

A novel fluorescent strategy for transcription factors assay was developed based on protein binding-protected DNA three-way junction-mediated rolling circle amplification.

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