scholarly journals Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification

2008 ◽  
Vol 37 (3) ◽  
pp. e19-e19 ◽  
Author(s):  
Taku Murakami ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Nucleic Acid Sequence ◽  
Rolling Circle

The analysis of proteins and small molecules based on sterically tunable nucleic acid hyperbranched rolling circle amplification

2017 ◽  
Vol 91 ◽  
pp. 136-142 ◽  
Author(s):  
Hai Shi ◽  
Xiaoxia Mao ◽  
Xiaoxia Chen ◽  
Zihan Wang ◽  
Keming Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Small Molecules ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Mitigating the Non-Specific Amplification in RCA: Assessment of the Role of Ligation and Exonuclease Digestion in Circular DNA Preparation

2021 ◽  
Author(s):  
Vandana Kuttappan Nair ◽  
Chandrika Sharma ◽  
Mrittika Sengupta ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
False Positive ◽  
Rolling Circle Amplification ◽  
Circular Dna ◽  
Rolling Circle ◽  
Specific Amplification ◽  
Exonuclease Digestion ◽  
Sticky End

<b>Layman Summary: </b>Rolling circle amplification (RCA) is a popular and extensively used bioanalytical tool. Like any nucleic acid amplifications, non-specific amplification may occur in it and risk generating false positive readouts. The work described in the manuscript investigates non-specific amplification in RCA as a function of ligation and exonuclease digestion assays during the synthesis of circular DNA. In particular, it investigates and compares the role of three different ligation techniques, namely splint-padlock ligation, cohesive end (sticky end ligation), and self-annealing ligation. In addition, it also probes the role of single exonuclease vs dual exonuclease digestions. We employed real time fluorescence to quantify the effect of these factors. Finally, our work hypothesizes the possible origins of non-specific amplification in RCA.

Nucleic acid sensing by regenerable surface-associated isothermal rolling circle amplification

2007 ◽  
Vol 22 (7) ◽  
pp. 1236-1244 ◽  
Author(s):  
Erik L. McCarthy ◽  
Lee E. Bickerstaff ◽  
Mauricio Pereira da Cunha ◽  
Paul J. Millard
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Rolling Circle

scholarly journals Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 352
Author(s):  
Kuankuan Zhang ◽  
Hua Zhang ◽  
Haorui Cao ◽  
Yu Jiang ◽  
Kang Mao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Environmental Pollutants ◽  
Monitoring Method ◽  
Rolling Circle ◽  
Advantages And Disadvantages ◽  
Contaminant Monitoring ◽  
Aqueous Environments ◽  
Global Concern ◽  
Circular Template

Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring.

Mitigating the Non-Specific Amplification in RCA: Assessment of the Role of Ligation and Exonuclease Digestion in Circular DNA Preparation

2021 ◽  
Author(s):  
Vandana Kuttappan Nair ◽  
Chandrika Sharma ◽  
Mrittika Sengupta ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
False Positive ◽  
Rolling Circle Amplification ◽  
Circular Dna ◽  
Rolling Circle ◽  
Specific Amplification ◽  
Exonuclease Digestion ◽  
Sticky End

<b>Layman Summary: </b>Rolling circle amplification (RCA) is a popular and extensively used bioanalytical tool. Like any nucleic acid amplifications, non-specific amplification may occur in it and risk generating false positive readouts. The work described in the manuscript investigates non-specific amplification in RCA as a function of ligation and exonuclease digestion assays during the synthesis of circular DNA. In particular, it investigates and compares the role of three different ligation techniques, namely splint-padlock ligation, cohesive end (sticky end ligation), and self-annealing ligation. In addition, it also probes the role of single exonuclease vs dual exonuclease digestions. We employed real time fluorescence to quantify the effect of these factors. Finally, our work hypothesizes the possible origins of non-specific amplification in RCA.

A netlike rolling circle nucleic acid amplification technique

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Xiaoli Zhu ◽  
Chang Feng ◽  
Bin Zhang ◽  
Hui Tong ◽  
Tao Gao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Nucleic Acid Amplification ◽  
Amplification Efficiency ◽  
Rolling Circle ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Technique ◽  
Nucleic Acid Amplification Technique ◽  
Network Morphology

An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.

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