Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering

2018 ◽  
Vol 6 (3) ◽  
pp. 550-561 ◽  
Author(s):  
Koji Nagahama ◽  
Naho Oyama ◽  
Kimika Ono ◽  
Atsushi Hotta ◽  
Keiko Kawauchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Building Blocks ◽  
The Body ◽  
Bioactive Molecules ◽  
In Vivo Tissue Engineering ◽  
Injectable Gels

Nanocomposite injectable gels, which self-replenish regenerative extracellular microenvironments within the gels in the body by utilizing host-derived bioactive molecules as building blocks, are reported.

scholarly journals Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

2021 ◽  
Vol 8 (3) ◽  
pp. 39
Author(s):  
Britani N. Blackstone ◽  
Summer C. Gallentine ◽  
Heather M. Powell
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Collagen Type I ◽  
The Body ◽  
Pool Data ◽  
Type I ◽  
High Concentrations ◽  
Increased Strength

Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

The use of pimonidazole to characterise hypoxia in the internal environment of an in vivo tissue engineering chamber

2005 ◽  
Vol 58 (8) ◽  
pp. 1104-1114 ◽  
Author(s):  
S.O.P. Hofer ◽  
G.M. Mitchell ◽  
A.J. Penington ◽  
W.A. Morrison ◽  
R. RomeoMeeuw ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Internal Environment ◽  
In Vivo Tissue Engineering

scholarly journals The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

2018 ◽  
Vol 59 (3-4) ◽  
pp. 286-299 ◽  
Author(s):  
Annika Weigand ◽  
Raymund E. Horch ◽  
Anja M. Boos ◽  
Justus P. Beier ◽  
Andreas Arkudas
Keyword(s):  
Tissue Engineering ◽  
Large Scale ◽  
Treatment Options ◽  
Current Treatment ◽  
Loop Model ◽  
Engineering Approach ◽  
In Vivo Tissue Engineering ◽  
Tissue Engineering Approach ◽  
Tissue Defects

Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects.

TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

2009 ◽  
Vol 21 (03) ◽  
pp. 149-155 ◽  
Author(s):  
Hsu-Wei Fang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Bone Cells ◽  
Cartilage Tissue Engineering ◽  
Bioactive Molecules ◽  
In Vivo Studies ◽  
Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

scholarly journals Clinical Approaches of Biomimetic: An Emerging Next Generation Technology

2021 ◽  
Author(s):  
Kirti Rani
Keyword(s):  
Tissue Engineering ◽  
Close Contact ◽  
The Body ◽  
Next Generation ◽  
Biochemical Engineering ◽  
Security Systems ◽  
Material Sciences ◽  
Reported Data ◽  
Generation Technology

Biomimetic is the study of various principles of working mechanisms of naturally occurring phenomena and their further respective integrations in to such a modified advanced mechanized instruments/models of digital or artificial intelligence protocols. Hence, biomimetic has been proposed in last decades for betterment of human mankind for improving security systems by developing various convenient robotic vehicles and devices inspired by natural working phenomenon of plants, animals, birds and insects based on biochemical engineering and nanotechnology. Hence, biomimetic will be considered next generation technology to develop various robotic products in the fields of chemistry, medicine, material sciences, regenerative medicine and tissue engineering medicine, biomedical engineering to treat various diseases and congenital disorders. The characteristics of tissue engineered scaffolds are found to possess multifunctional cellular properties like biocompatibility, biodegradability and favorable mechanized properties when comes in close contact with the body fluids in vivo. This chapter will provide overall overview to the readers for the study based on reported data of developed biomimetic materials and tools exploited for various biomedical applications and tissue engineering applications which further helpful to meet the needs of the medicine and health care industries.

scholarly journals In vivo Tissue Engineering: Mimicry of Homing Factors for Self-Endothelialization of Blood-Contacting Materials

Pathobiology ◽  
2013 ◽  
Vol 80 (4) ◽  
pp. 176-181 ◽  
Author(s):  
Meltem Avci-Adali ◽  
Heidi Stoll ◽  
Nadja Wilhelm ◽  
Nadja Perle ◽  
Christian Schlensak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vivo Tissue Engineering

Optimization of Oxygen Delivery within Hydrogels

2021 ◽  
Author(s):  
Sophia M Mavris ◽  
Laura M Hansen
Keyword(s):  
Tissue Engineering ◽  
Oxygen Transport ◽  
Current Medical ◽  
The Body ◽  
Clinical Translation ◽  
Technological Innovations ◽  
Technological Advances ◽  
Cell Sources

Abstract The field of tissue engineering has been continuously evolving since its inception over three decades ago with numerous new advancements in biomaterials and cell sources and widening applications to most tissues in the body. Despite the substantial promise and great opportunities for the advancement of current medical therapies and procedures, the field has yet to capture wide clinical translation due to some remaining challenges, including oxygen availability within constructs, both in vitro and in vivo. While this insufficiency of nutrients, specifically oxygen, is a limitation within the current frameworks of this field, the literature shows promise in new technological advances to efficiently provide adequate delivery of nutrients to cells. This review attempts to capture the most recent advances in the field of oxygen transport in hydrogel-based tissue engineering, including a comparison of current research as it pertains to the modeling, sensing, and optimization of oxygen within hydrogel constructs as well as new technological innovations to overcome traditional diffusion-based limitations. The application of these findings can further the advancement and development of better hydrogel-based tissue engineered constructs for future clinical translation and adoption.

Reconstructing Box-Like Bone Defect of Mandible with In Vivo Tissue Engineering Bone

2007 ◽  
pp. 1165-1168
Author(s):  
Jin Feng Yao ◽  
Y.Z. Zhang ◽  
C.Y. Bao ◽  
L.Y. Sun ◽  
X.M. Hao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Defect ◽  
In Vivo Tissue Engineering
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