Time-resolved fluorescence-based assay for rapid detection of Escherichia coli

2015 ◽  
Vol 470 ◽  
pp. 1-6 ◽  
Author(s):  
Janne Kulpakko ◽  
Kari Kopra ◽  
Pekka Hänninen
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Detection of Escherichia coli rRNA using target amplification and time-resolved fluorescence detection

1991 ◽  
Vol 5 (6) ◽  
pp. 467-472 ◽  
Author(s):  
Charlene E. Bush ◽  
Kurt M. Vanden Brink ◽  
David G. Sherman ◽  
W. Richard Peterson ◽  
Laura A. Beninsig ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Sensitive and simple detection of Escherichia coli strain based on time-resolved fluorescence DNA hybridization assay

2010 ◽  
Vol 664 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Min Ruan ◽  
Cheng-Gang Niu ◽  
Pin-Zhu Qin ◽  
Guang-Ming Zeng ◽  
Zhao-Hui Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Hybridization ◽  
Coli Strain ◽  
Hybridization Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Simple Detection

A Time-Resolved-Fluorescence Lateral Flow Assay for Rapid Detection of Cholyglycine Acid for the Diagnosis of Liver Diseases

2021 ◽  
Vol 11 (10) ◽  
pp. 2023-2029
Author(s):  
Wang Zhang ◽  
Yujun Zhou ◽  
Xiuli Xu ◽  
Yaping Tian ◽  
Chunyan Zhang
Keyword(s):  
Rapid Detection ◽  
Liver Diseases ◽  
Point Of Care ◽  
Quantitative Information ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Homogeneous Enzyme Immunoassay

Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum, plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.

Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

2017 ◽  
Vol 9 (45) ◽  
pp. 6430-6434 ◽  
Author(s):  
Yi Zhang ◽  
Xin-Liang Ding ◽  
Ming-Ming Guo ◽  
Ting-Ting Han ◽  
Zi-Jian Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

In the present study, a novel time-resolved fluorescence immunochromatographic assay was established for the rapid quantitative detection of microcystin-leucine arginine (MC-LR).

Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B1

2020 ◽  
Vol 12 (43) ◽  
pp. 5229-5235
Author(s):  
Lingling Guo ◽  
Zhongxing Wang ◽  
Xinxin Xu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Rapid Detection ◽  
Detection Method ◽  
Fumonisin B1 ◽  
Immunochromatographic Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The developed time-resolved fluorescence immunochromatographic assay can be widely applicable as an ultrasensitive, rapid detection method for FB1 in grains.

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