scholarly journals Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase

2018 ◽  
Vol 9 (28) ◽  
pp. 6053-6061 ◽  
Author(s):  
Li-juan Wang ◽  
Xiao Han ◽  
Chen-chen Li ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Specific Detection ◽  
Mtase Activity

Specific and sensitive detection of DNA MTase activity can be achieved by a single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification approach.

Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification

2015 ◽  
Vol 51 (73) ◽  
pp. 13968-13971 ◽  
Author(s):  
Yan Zhang ◽  
Wen-jing Xu ◽  
Ya-ping Zeng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Methyltransferase Activity

We develop transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification for sensitive detection of DNA methyltransferase activity.

Detection of DNA methyltransferase activity using allosteric molecular beacons

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 579-584 ◽  
Author(s):  
Weiting Zhang ◽  
Xiaolong Zu ◽  
Yanling Song ◽  
Zhi Zhu ◽  
Chaoyong James Yang
Keyword(s):  
Dna Methylation ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Sensitive Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Adenine Methylation ◽  
Methylation Patterns

Abnormal DNA methylation patterns caused by altered DNA methyltransferase (MTase) activity are closely associated with cancer. Herein, using DNA adenine methylation methyltransferase (Dam MTase) as a model analyte, we designed an allosteric molecular beacon (aMB) for sensitive detection of Dam MTase activity.

A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification

2017 ◽  
Vol 9 (20) ◽  
pp. 2933-2938 ◽  
Author(s):  
Ying Chen ◽  
Hongchao Yi
Keyword(s):  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Enzyme Amplification ◽  
Signal Output

The polymerization nicking reaction (PNR) and invertase enzyme amplify the glucometer signal output to achieve simple and sensitive detection of DNA methyltransferase (MTase) activity.

A highly sensitive fluorescence biosensor for detection of Staphylococcus aureus based on HCR-mediated three-way DNA junction nicking enzyme assisted signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Chuyan Zhang ◽  
Zewei Luo ◽  
Mengfan Wu ◽  
Wei Ning ◽  
Ziyi Tian ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Safety ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Safety Control ◽  
Food Borne ◽  
Highly Sensitive ◽  
Fluorescence Biosensor ◽  
Highly Sensitive Detection ◽  
Nicking Enzyme

Sensitive and efficient monitoring of food-borne bacteria is of great importance for food safety control. Herein, a novel biosensor for highly sensitive detection of Staphylococcus aureus (S. aureus) was constructed...

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