Rapid simultaneous extraction and magnetic particle-based enzyme immunoassay for the parallel determination of ochratoxin A, fumonisin B1 and deoxynivalenol mycotoxins in cereal samples

2017 ◽  
Vol 9 (24) ◽  
pp. 3602-3611 ◽  
Author(s):  
J. C. Vidal ◽  
J. R. Bertolín ◽  
A. Ezquerra ◽  
S. Hernández ◽  
J. R. Castillo
Keyword(s):  
Enzyme Immunoassay ◽  
Ochratoxin A ◽  
Rapid Method ◽  
Magnetic Particle ◽  
Fumonisin B1 ◽  
Simultaneous Extraction ◽  
Immunochemical Determination ◽  
Cereal Samples

This work describes the development of a rapid method for the extraction and the immunochemical determination of three mycotoxins, deoxynivalenol, fumonisin B1, and ochratoxin A, from cereal samples (wheat and corn flours).

Determination of Pentachlorophenol in Water and Soil by a Magnetic Particle-Based Enzyme Immunoassay

1995 ◽  
Vol 29 (11) ◽  
pp. 2754-2758 ◽  
Author(s):  
Charles S. Hottenstein ◽  
Scott W. Jourdan ◽  
Mary C. Hayes ◽  
Fernando M. Rubio ◽  
David P. Herzog ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

Determination of Trace Atrazine Levels in Water by a Sensitive Magnetic Particle-Based Enzyme Immunoassay

1996 ◽  
Vol 44 (11) ◽  
pp. 3576-3581 ◽  
Author(s):  
Charles S. Hottenstein ◽  
Fernando M. Rubio ◽  
David P. Herzog ◽  
James R. Fleeker ◽  
Timothy S. Lawruk
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

Simultaneous Detection of Ochratoxin A and Fumonisin B1 in Cereal Samples Using an Aptamer–Photonic Crystal Encoded Suspension Array

2014 ◽  
Vol 86 (23) ◽  
pp. 11797-11802 ◽  
Author(s):  
Sun Yue ◽  
Xu Jie ◽  
Li Wei ◽  
Cao Bin ◽  
Wang Dou Dou ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Ochratoxin A ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Suspension Array ◽  
Cereal Samples

Development of a Magnetic Particle-Based Enzyme Immunoassay for the Determination of Penoxsulam in Water

2008 ◽  
Vol 56 (17) ◽  
pp. 7606-7612 ◽  
Author(s):  
Daniel R. Rubio ◽  
Lisa M. Kamp ◽  
Mark Heilman ◽  
Lee Williams ◽  
Fernando M. Rubio
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

Comparison of single and multi-analyte methods based on LC-MS/MS for mycotoxin biomarker determination in human urine

2013 ◽  
Vol 6 (4) ◽  
pp. 355-366 ◽  
Author(s):  
M. Solfrizzo ◽  
L. Gambacorta ◽  
B. Warth ◽  
K. White ◽  
C. Srey ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fumonisin B1 ◽  
Good Method ◽  
Aflatoxin M1 ◽  
Z Score ◽  
Future Studies ◽  
Naturally Occurring ◽  
Z Scores ◽  
Calibration Solution

The performances of four LC-MS/MS methodologies for determination of up to eight mycotoxin biomarkers in human urines were compared by involving three laboratories that analysed common urine samples spiked at two levels of each biomarker. Each laboratory received a calibration solution, spiked urines and the corresponding unspiked urine. The two spiking levels for each biomarker were chosen by considering the levels naturally occurring in human urines and the limits of quantification of the LC-MS/MS methodologies used by the participating laboratories. The results of each laboratory were evaluated for their z-score values. The percentage of satisfactory z-scores (| z | < 2) were: 100% for deoxynivalenol, de-epoxy deoxynivalenol, aflatoxin M1, β-zearalenol and zearalenone, 87% for α-zearalenol, 50% for ochratoxin A and 42% for fumonisin B1. Good method performances were obtained for most biomarkers at the levels tested in this study, as demonstrated by the overall percentage of satisfactory z-scores for all analytes (87%). Unsatisfactory/questionable z-scores (| z | ≯2) were obtained for fumonisin B1 (7/12 results), ochratoxin A (4/8 results) and ?-zearalenol (1/8 results). The percentage of satisfactory z-scores for fumonisin B1 and ochratoxin A increased from 42 to 83% for fumonisin B1 and from 50 to 62% for ochratoxin A when laboratories 1 and 2 used own calibrants. Factors that could explain the different results obtained for fumonisin B1 and ochratoxin A with provided and own calibration solutions could not be identified in this study and should be carefully investigated in future studies.

A Magnetic Particle-Based Competitive Enzyme Immunoassay for Rapid Determination of Ciprofloxacin: A Potential Method for the General Detection of Fluoroquinolones

2014 ◽  
Vol 47 (7) ◽  
pp. 1134-1146 ◽  
Author(s):  
Bo Zhang ◽  
Daolin Du ◽  
Meng Meng ◽  
Sergei A. Eremin ◽  
Victor B. Rybakov ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle ◽  
Rapid Determination ◽  
Potential Method

Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

1995 ◽  
Vol 43 (5) ◽  
pp. 1413-1419 ◽  
Author(s):  
Timothy S. Lawruk ◽  
Adrian M. Gueco ◽  
Scott W. Jourdan ◽  
Adele M. Scutellaro ◽  
James R. Fleeker ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle ◽  
Agricultural Products

Ultrasonic Extraction with Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry for the Determination of Ochratoxin A in Processed Cereal Products

2014 ◽  
Vol 97 (5) ◽  
pp. 1384-1386 ◽  
Author(s):  
Kieu Thi Ngoc Nguyen ◽  
Dojin Ryu
Keyword(s):  
Mass Spectrometry ◽  
Ochratoxin A ◽  
Reliable Method ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Cereal Products ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Cereal Samples

Abstract A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed for determination of ochratoxin A (OTA) in processed cereal products. OTA was ultrasonically extracted from the sample with acetonitrile–water (80 + 20, v/v), and the extract was then injected into the UPLC/MS/MS system after filtration. The calibration curves had good linearity with coefficients of determination greater than 0.999. Recoveries of OTA were in the range of 90–104%. LOD and LOQ of OTA in samples were 0.6 and 2.0 ng/g, respectively, and no significant matrix effect was found. This method was applied to determine OTA in 25 oat-based cereal samples. OTA was detected in five samples (20%) in the range of 2.4 to 7.3 ng/g.

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