Determination of Pentachlorophenol in Water and Soil by a Magnetic Particle-Based Enzyme Immunoassay

1995 ◽  
Vol 29 (11) ◽  
pp. 2754-2758 ◽  
Author(s):  
Charles S. Hottenstein ◽  
Scott W. Jourdan ◽  
Mary C. Hayes ◽  
Fernando M. Rubio ◽  
David P. Herzog ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

Determination of Trace Atrazine Levels in Water by a Sensitive Magnetic Particle-Based Enzyme Immunoassay

1996 ◽  
Vol 44 (11) ◽  
pp. 3576-3581 ◽  
Author(s):  
Charles S. Hottenstein ◽  
Fernando M. Rubio ◽  
David P. Herzog ◽  
James R. Fleeker ◽  
Timothy S. Lawruk
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

Rapid simultaneous extraction and magnetic particle-based enzyme immunoassay for the parallel determination of ochratoxin A, fumonisin B1 and deoxynivalenol mycotoxins in cereal samples

2017 ◽  
Vol 9 (24) ◽  
pp. 3602-3611 ◽  
Author(s):  
J. C. Vidal ◽  
J. R. Bertolín ◽  
A. Ezquerra ◽  
S. Hernández ◽  
J. R. Castillo
Keyword(s):  
Enzyme Immunoassay ◽  
Ochratoxin A ◽  
Rapid Method ◽  
Magnetic Particle ◽  
Fumonisin B1 ◽  
Simultaneous Extraction ◽  
Immunochemical Determination ◽  
Cereal Samples

This work describes the development of a rapid method for the extraction and the immunochemical determination of three mycotoxins, deoxynivalenol, fumonisin B1, and ochratoxin A, from cereal samples (wheat and corn flours).

Development of a Magnetic Particle-Based Enzyme Immunoassay for the Determination of Penoxsulam in Water

2008 ◽  
Vol 56 (17) ◽  
pp. 7606-7612 ◽  
Author(s):  
Daniel R. Rubio ◽  
Lisa M. Kamp ◽  
Mark Heilman ◽  
Lee Williams ◽  
Fernando M. Rubio
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle

A Magnetic Particle-Based Competitive Enzyme Immunoassay for Rapid Determination of Ciprofloxacin: A Potential Method for the General Detection of Fluoroquinolones

2014 ◽  
Vol 47 (7) ◽  
pp. 1134-1146 ◽  
Author(s):  
Bo Zhang ◽  
Daolin Du ◽  
Meng Meng ◽  
Sergei A. Eremin ◽  
Victor B. Rybakov ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle ◽  
Rapid Determination ◽  
Potential Method

Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

1995 ◽  
Vol 43 (5) ◽  
pp. 1413-1419 ◽  
Author(s):  
Timothy S. Lawruk ◽  
Adrian M. Gueco ◽  
Scott W. Jourdan ◽  
Adele M. Scutellaro ◽  
James R. Fleeker ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particle ◽  
Agricultural Products

scholarly journals AB0110 DETERMINING THE AMOUNT OF COPPER–CONTAINING PROTEIN, ITS BIOCHEMICAL AND IMMUNOLOGIC ACTIVITY IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1083.2-1084
Author(s):  
O. Rusanova ◽  
A. Trofimenko ◽  
N. Emelyanov ◽  
O. Emelyanova
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Enzyme Immunoassay ◽  
Oxidase Activity ◽  
Mean Value ◽  
Hospital Treatment ◽  
Diagnostic Agent ◽  
Immunologic Activity ◽  
Rheumatoid Arthritis Activity

Background:Production of antibodies to ceruloplasmin (CP) in rheumatoid arthritis is an issue that has not been studied well enough. It was not by chance that this copper–containing alpha 2-glycorpoteid of blood plasma showing multienzymatic properties was chosen as an object of investigation. Data on the content and activity of CP in the blood of rheumatoid arthritis patients are contradictory, which has to do with different approaches to selection of patients and different measuring methods.Objectives:Improving diagnosis of rheumatoid arthritis by determination of antibodies to CP as well as its amount and enzymatic activity.Methods:We studied the serum from 30 apparently healthy individuals, and 108 rheumatoid arthritis patients. Antibodies to CP were determined by enzyme immunoassay using immobilized granulated antigen preparations (modification by Gontar et al, 2002). The amount of CP was determined by enzyme immunoassay according to the method of I.S. Kuzmina et al (1991) using commercial diagnostic agent manufactured by Mechnikov Research Institute for Vaccines and Sera.Results:Enzyme immunoassay showed a mean level of CP antibodies in donor sera of 0,020±0,006 optical density units. The level of normal values of specific antibodies determined as M±2σ included an extinction value in the range 0 – 0,086. The mean value of oxidase activity and the amount of CP in healthy people was 716±26,3 and 921±32 ng/ml, correspondingly. In the process of study we revealed a reliable increase in CP antibody count, the activity and amount of CP in patients with rheumatoid arthritis while in all cases the parameters under study correlated with the degree of disease activity (p<0,05): at activity degree I CP antibodies were 0,098±0,011; CP activity was 954±48,1; CP amount was 1292±73,4. At activity degree II CP antibodies were 0,138±0,007; CP activity was 1163±39,6; CP amount was 1763±69,3. At activity degree III, CP antibodies were 0,182±0,015; CP activity was 1368±89,5; CP amount was 1794±102,8. After a course of hospital treatment was completed, we noted a reliable decrease in the activity and amount of CP (at degree I of rheumatoid arthritis activity p<0,001, at degree II of rheumatoid arthritis activity p<0,01for both parameters; at degree III, p<0,05) compared with baseline findings. A decrease in CP antibodies shows decelerated dynamics, especially in patients with pronounced disease activity, which indicates severe disorders in the immunity that cannot be cured completely within 30 – 40 days of hospital treatment course.Conclusion:Determination of CP antibodies, as well as quantitative content of CP and its oxidase activity can serve as indicators of the activity of rheumatoid arthritis, as well as an accessory criterion of the effectiveness of administered therapy.Disclosure of Interests:None declared

scholarly journals Magnetic particle characterization in the Seine river system: Implications for the determination of natural versus anthropogenic input

2009 ◽  
Vol 10 (8) ◽  
pp. n/a-n/a ◽  
Author(s):  
Christine Franke ◽  
Catherine Kissel ◽  
Eric Robin ◽  
Philippe Bonté ◽  
France Lagroix
Keyword(s):  
Magnetic Particle ◽  
River System ◽  
Particle Characterization ◽  
Anthropogenic Input ◽  
Seine River

Measurement of progesterone in sheep using a commercial ELISA kit for human plasma

2021 ◽  
pp. 104063872110435
Author(s):  
Valeria Pasciu ◽  
Maria Nieddu ◽  
Elena Baralla ◽  
Cristian Porcu ◽  
Francesca Sotgiu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Human Plasma ◽  
Method Validation ◽  
Plasma Progesterone ◽  
Freeze Thaw ◽  
Elisa Kit ◽  
Stability Data ◽  
Human Use ◽  
Species Specific

Determination of serum or plasma progesterone (P4) concentrations is important to recognize pregnant and non-pregnant ewes, and also to predict the number of carried lambs. The 2 most common methodologies for the detection of plasma P4 are radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA is very expensive, and not all laboratories are equipped to perform this test; EIA is commercially available for human use, but only a few companies produce species-specific kits, which are expensive. We verified for ovine plasma a less expensive and easily available ELISA kit (DiaMetra) designed to quantify P4 in humans. Pools of ovine and human plasma were used to compare repeatability, accuracy, sensitivity, and stability of P4 measured by the DiaMetra kit. Repeatability data were within 15%, and accuracy values were ~90% for both plasma matrices. Stability data showed a loss of <20% for freeze–thaw and <30% for 30-d storage. All parameters were acceptable under international guidelines for method validation. The human ELISA kit was used successfully to quantify plasma P4 in 26 ewes during pregnancy until delivery. P4 concentrations were also correlated with the number of carried lambs.

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