Simultaneous Detection of Ochratoxin A and Fumonisin B1 in Cereal Samples Using an Aptamer–Photonic Crystal Encoded Suspension Array

2014 ◽  
Vol 86 (23) ◽  
pp. 11797-11802 ◽  
Author(s):  
Sun Yue ◽  
Xu Jie ◽  
Li Wei ◽  
Cao Bin ◽  
Wang Dou Dou ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Ochratoxin A ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Suspension Array ◽  
Cereal Samples

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

Rapid simultaneous extraction and magnetic particle-based enzyme immunoassay for the parallel determination of ochratoxin A, fumonisin B1 and deoxynivalenol mycotoxins in cereal samples

2017 ◽  
Vol 9 (24) ◽  
pp. 3602-3611 ◽  
Author(s):  
J. C. Vidal ◽  
J. R. Bertolín ◽  
A. Ezquerra ◽  
S. Hernández ◽  
J. R. Castillo
Keyword(s):  
Enzyme Immunoassay ◽  
Ochratoxin A ◽  
Rapid Method ◽  
Magnetic Particle ◽  
Fumonisin B1 ◽  
Simultaneous Extraction ◽  
Immunochemical Determination ◽  
Cereal Samples

This work describes the development of a rapid method for the extraction and the immunochemical determination of three mycotoxins, deoxynivalenol, fumonisin B1, and ochratoxin A, from cereal samples (wheat and corn flours).

Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array

2016 ◽  
Vol 458 ◽  
pp. 72-77 ◽  
Author(s):  
Xin Kong ◽  
Baofen Ye ◽  
Zixue Yang ◽  
Baoan Chen ◽  
Yun Ling
Keyword(s):  
Photonic Crystal ◽  
Simultaneous Detection ◽  
Specific Antibodies ◽  
Suspension Array

AN IMMUNOARRAY FOR THE SIMULTANEOUS DETECTION OF TWO MYCOTOXINS, OCHRATOXIN A AND FUMONISIN B1

2011 ◽  
Vol 31 (3) ◽  
pp. 408-416 ◽  
Author(s):  
XICHUN WANG ◽  
HAIBIN ZHANG ◽  
HAIMING LIU ◽  
CHENGHUA HE ◽  
AIHUA ZHANG ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Simultaneous Detection ◽  
Fumonisin B1

scholarly journals Detection of Fumonisin B1 and Ochratoxin A in Grain Products Using Microsphere-Based Fluid Array Immunoassays

Toxins ◽  
2010 ◽  
Vol 2 (2) ◽  
pp. 297-309 ◽  
Author(s):  
George P. Anderson ◽  
Vasudha A. Kowtha ◽  
Chris R. Taitt
Keyword(s):  
Ochratoxin A ◽  
Fumonisin B1 ◽  
Grain Products

scholarly journals Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglets

2013 ◽  
Vol 6 (3) ◽  
pp. 299-308 ◽  
Author(s):  
S. Gambacorta ◽  
H. Solfrizzo ◽  
A. Visconti ◽  
S. Powers ◽  
A.M. Cossalter ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Dose Response ◽  
Fumonisin B1 ◽  
Correlation Coefficients ◽  
Urinary Biomarkers ◽  
Post Dose ◽  
Toxigenic Fungi ◽  
Biomarkers Of Exposure ◽  
Commission Recommendation ◽  
Aflatoxin B

The multi-biomarker approach was used to validate urinary biomarkers in piglets administered boluses contaminated with mixtures of deoxynivalenol (DON), aflatoxin B1 (AFB1), fumonisin B1 (FB1), zearalenone (ZEA) and ochratoxin A (OTA) at different concentrations. Boluses contaminated with mycotoxins were prepared by slurrying and freezedrying feed material fortified with culture extracts of selected toxigenic fungi. Piglets were individually placed in metabolic cages to collect urine before gavage and 24 h post dose. Urine samples were hydrolysed with β-glucuronidase and analysed by a multi-biomarker LC-MS/MS method developed and validated to identify and measure biomarkers of FB1, OTA, DON, ZEA and AFB1. Urinary levels of FB1, OTA, DON + de-epoxy-deoxynivalenol, ZEA + alphazearalenol and aflatoxin M1 were selected as biomarkers of FB1, OTA, DON, ZEA and AFB1, respectively. Mean percentages of dietary mycotoxins excreted as biomarkers in 24 h post dose urine were 36.8% for ZEA, 28.5% for DON, 2.6% FB1, 2.6% for OTA and 2.5% for AFB1. A good correlation was observed between the amount of mycotoxins ingested and the amount of relevant biomarkers excreted in 24 h post dose urine. Linear dose-response correlation coefficients ranged between 0.68 and 0.78 for the tested couples of mycotoxin/biomarker. The good sensitivity of the LC-MS/MS method and the good dose-response correlations observed in this study permitted to validate the selected mycotoxin biomarkers in piglets at dietary levels close to the maximum permitted levels reported in Commission Directive 2003/100/EC for AFB1 and the guidance values reported in Commission Recommendation 2006/576/EC for DON, ZEA, OTA and FB1.

Sign in / Sign up

Export Citation Format

Share Document