scholarly journals Highly specific C–C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging

2017 ◽  
Vol 8 (3) ◽  
pp. 2199-2203 ◽  
Author(s):  
Hua Li ◽  
Deliang Zhang ◽  
Mengna Gao ◽  
Lumei Huang ◽  
Longguang Tang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Bond Cleavage ◽  
Quantitative Imaging ◽  
Dihydropyridine Derivatives

A novel FRET fluorescence “off–on” system based on the highly specific, sensitive and effective C–C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO).

scholarly journals In vivo characterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid

2012 ◽  
Vol 4 (2) ◽  
pp. 550 ◽  
Author(s):  
Niall J. Finnerty ◽  
Saidhbhe L. O'Riordan ◽  
Finbar O. Brown ◽  
Pier A. Serra ◽  
Robert D. O'Neill ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Extracellular Fluid ◽  
Pt Electrode ◽  
Brain Extracellular Fluid

Mechanisms of polymicrobial sepsis-induced ileus

2004 ◽  
Vol 287 (3) ◽  
pp. G685-G694 ◽  
Author(s):  
Marcus Overhaus ◽  
Sandra Tögel ◽  
Michael A. Pezzone ◽  
Anthony J. Bauer
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Real Time ◽  
Inducible Nitric Oxide Synthase ◽  
Gastrointestinal Transit ◽  
Polymicrobial Sepsis ◽  
Rt Pcr ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Sepsis frequently occurs after hemorrhage, trauma, burn, or abdominal surgery and is a leading cause of morbidity and mortality in severely ill patients. We performed experiments to delineate intestinal molecular and functional motility consequences of polymicrobial sepsis in the clinically relevant cecal ligation and puncture (CLP) sepsis model. CLP was performed on male Sprague-Dawley rats. Gastrointestinal transit, colonic in vivo pressure recordings, and in vitro muscle contractions were recorded. Histochemistry was performed for macrophages, monocytes, and neutrophils. Inflammatory gene expressions were quantified by real-time RT-PCR. CLP delayed gastrointestinal transit, decreased colonic pressures, and suppressed in vivo circular muscle contractility of the jejunum and colon over a 4-day period. A leukocytic infiltrate of monocytes and neutrophils developed over 24 h. Real-time RT-PCR demonstrated a significant temporal elevation in IL-6, IL-1β, monocyte chemoattractant protein-1, and inducible nitric oxide synthase, with higher expression levels of IL-6 and inducible nitric oxide synthase in colonic extracts compared with small intestine. Polymicrobial CLP sepsis induces a complex inflammatory response within the intestinal muscularis with the recruitment of leukocytes and elaboration of mediators that inhibit intestinal muscle function. Differences were elucidated between endotoxin and CLP models of sepsis, as well as a heterogeneous regional response of the gastrointestinal tract to CLP. Thus the intestine is not only a source of bacteremia but also an important target of bacterial products with major functional consequences to intestinal motility and the generation of cytokines, which participate in the development of multiple organ failure.

Organic semiconducting nanoprobe with redox-activatable NIR-II fluorescence forin vivoreal-time monitoring of drug toxicity

2019 ◽  
Vol 55 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Yufu Tang ◽  
Yuanyuan Li ◽  
Zhen Wang ◽  
Feng Pei ◽  
Xiaoming Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Drug Toxicity ◽  
Drug Dose ◽  
Non Invasive ◽  
Dose Dependent

A nitric-oxide-activatable organic semiconducting nanoprobe was developed forin vivo,in situ, real-time and non-invasive NIR-II fluorescence monitoring of drug-dose-dependent hepatotoxicity.

Origin of endogenous nitric oxide released in the nucleus accumbens under real-time in vivo conditions

Life Sciences ◽  
2015 ◽  
Vol 134 ◽  
pp. 79-84 ◽  
Author(s):  
Helmut Prast ◽  
Ariane Hornick ◽  
Michaela M. Kraus ◽  
Athineos Philippu
Keyword(s):  
Nitric Oxide ◽  
Nucleus Accumbens ◽  
Real Time ◽  
Endogenous Nitric Oxide

The real-time in vivo electrochemical measurement of nitric oxide and carbon monoxide release upon direct epidural electrical stimulation of the rat neocortex

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3415-3421 ◽  
Author(s):  
Sarah S. Park ◽  
Minyoung Hong ◽  
Yejin Ha ◽  
Jeongeun Sim ◽  
Gil-Ja Jhon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carbon Monoxide ◽  
Electrical Stimulation ◽  
Real Time ◽  
Vascular Function ◽  
Electrochemical Measurement ◽  
Functional Measurement ◽  
Rat Neocortex ◽  
Stimulation Of

This study reports real-time,in vivofunctional measurement of nitric oxide (NO) and carbon monoxide (CO), two gaseous mediators in controlling vascular function.

Real-time profiling of kidney tubular fluid nitric oxide concentrations in vivo

2001 ◽  
Vol 281 (1) ◽  
pp. F189-F194 ◽  
Author(s):  
David Z. Levine ◽  
Michelle Iacovitti ◽  
Kevin D. Burns ◽  
Xueji Zhang ◽  
Keyword(s):  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Proximal Tubule ◽  
Real Time ◽  
Zero Point ◽  
Renal Ablation ◽  
Highly Sensitive ◽  
Early Proximal Tubule

To directly determine intratubular nitric oxide concentrations ([NO]) in vivo, we modified amperometric integrated electrodes (WPI P/N ISO-NOP007), which are highly sensitive to NO and not affected by ascorbic acid, nitrite, l-arginine, or dopamine. Although reactive lengths were as short as 5 μm long, the electrode still responded rapidly. With the use of kidney surface fluid as the “zero point,” the electrode tip was inserted into tubular segments along the track of a perforation made by a beveled glass pipette. The surface fluid zero point was usually stable as distal, late proximal, and early proximal tubule [NO] levels were measured sequentially in the same nephron. In eight normal rats, distal, late proximal, and early proximal [NO] concentrations were each ∼110 nM. In contrast, in nine 5/6 nephrectomized rats 2 wk postsurgery, although [NO] also did not differ among distal, late proximal, and early proximal segments, levels were approximately fourfold higher than those in normal rats and were significantly reduced after N G-monomethyl-l-arginine administration. These are the first quantitative in vivo tubular fluid [NO] measurements and show a significant increase in tubular fluid [NO] after renal ablation.

scholarly journals Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Mireia Perez-Camps ◽  
Jing Tian ◽  
Serene C Chng ◽  
Kai Pin Sem ◽  
Thankiah Sudhaharan ◽  
...  
Keyword(s):  
Real Time ◽  
Developmental Process ◽  
Quantitative Imaging ◽  
Quantitative Model ◽  
Correlation Spectroscopy ◽  
Bmp Signaling ◽  
Fluorescence Lifetime Imaging ◽  
Fluorescence Correlation ◽  
Lifetime Imaging

Formation of the three embryonic germ layers is a fundamental developmental process that initiates differentiation. How the zebrafish pluripotency factor Pou5f3 (homologous to mammalian Oct4) drives lineage commitment is unclear. Here, we introduce fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to assess the formation of Pou5f3 complexes with other transcription factors in real-time in gastrulating zebrafish embryos. We show, at single-cell resolution in vivo, that Pou5f3 complexes with Nanog to pattern mesendoderm differentiation at the blastula stage. Later, during gastrulation, Sox32 restricts Pou5f3–Nanog complexes to the ventrolateral mesendoderm by binding Pou5f3 or Nanog in prospective dorsal endoderm. In the ventrolateral endoderm, the Elabela / Aplnr pathway limits Sox32 levels, allowing the formation of Pou5f3–Nanog complexes and the activation of downstream BMP signaling. This quantitative model shows that a balance in the spatiotemporal distribution of Pou5f3–Nanog complexes, modulated by Sox32, regulates mesendoderm specification along the dorsoventral axis.

scholarly journals Real Time Microelectrode Measurement of Nitric Oxide in Kidney Tubular Fluid in vivo

Sensors ◽  
2003 ◽  
Vol 3 (8) ◽  
pp. 314-320 ◽  
Author(s):  
David Levine ◽  
Michelle Iacovitti
Keyword(s):  
Nitric Oxide ◽  
Real Time
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