scholarly journals In vivo characterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid

2012 ◽  
Vol 4 (2) ◽  
pp. 550 ◽  
Author(s):  
Niall J. Finnerty ◽  
Saidhbhe L. O'Riordan ◽  
Finbar O. Brown ◽  
Pier A. Serra ◽  
Robert D. O'Neill ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Extracellular Fluid ◽  
Pt Electrode ◽  
Brain Extracellular Fluid

Mechanisms of polymicrobial sepsis-induced ileus

2004 ◽  
Vol 287 (3) ◽  
pp. G685-G694 ◽  
Author(s):  
Marcus Overhaus ◽  
Sandra Tögel ◽  
Michael A. Pezzone ◽  
Anthony J. Bauer
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Real Time ◽  
Inducible Nitric Oxide Synthase ◽  
Gastrointestinal Transit ◽  
Polymicrobial Sepsis ◽  
Rt Pcr ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Sepsis frequently occurs after hemorrhage, trauma, burn, or abdominal surgery and is a leading cause of morbidity and mortality in severely ill patients. We performed experiments to delineate intestinal molecular and functional motility consequences of polymicrobial sepsis in the clinically relevant cecal ligation and puncture (CLP) sepsis model. CLP was performed on male Sprague-Dawley rats. Gastrointestinal transit, colonic in vivo pressure recordings, and in vitro muscle contractions were recorded. Histochemistry was performed for macrophages, monocytes, and neutrophils. Inflammatory gene expressions were quantified by real-time RT-PCR. CLP delayed gastrointestinal transit, decreased colonic pressures, and suppressed in vivo circular muscle contractility of the jejunum and colon over a 4-day period. A leukocytic infiltrate of monocytes and neutrophils developed over 24 h. Real-time RT-PCR demonstrated a significant temporal elevation in IL-6, IL-1β, monocyte chemoattractant protein-1, and inducible nitric oxide synthase, with higher expression levels of IL-6 and inducible nitric oxide synthase in colonic extracts compared with small intestine. Polymicrobial CLP sepsis induces a complex inflammatory response within the intestinal muscularis with the recruitment of leukocytes and elaboration of mediators that inhibit intestinal muscle function. Differences were elucidated between endotoxin and CLP models of sepsis, as well as a heterogeneous regional response of the gastrointestinal tract to CLP. Thus the intestine is not only a source of bacteremia but also an important target of bacterial products with major functional consequences to intestinal motility and the generation of cytokines, which participate in the development of multiple organ failure.

Organic semiconducting nanoprobe with redox-activatable NIR-II fluorescence forin vivoreal-time monitoring of drug toxicity

2019 ◽  
Vol 55 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Yufu Tang ◽  
Yuanyuan Li ◽  
Zhen Wang ◽  
Feng Pei ◽  
Xiaoming Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Drug Toxicity ◽  
Drug Dose ◽  
Non Invasive ◽  
Dose Dependent

A nitric-oxide-activatable organic semiconducting nanoprobe was developed forin vivo,in situ, real-time and non-invasive NIR-II fluorescence monitoring of drug-dose-dependent hepatotoxicity.

Origin of endogenous nitric oxide released in the nucleus accumbens under real-time in vivo conditions

Life Sciences ◽  
2015 ◽  
Vol 134 ◽  
pp. 79-84 ◽  
Author(s):  
Helmut Prast ◽  
Ariane Hornick ◽  
Michaela M. Kraus ◽  
Athineos Philippu
Keyword(s):  
Nitric Oxide ◽  
Nucleus Accumbens ◽  
Real Time ◽  
Endogenous Nitric Oxide

scholarly journals Highly specific C–C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging

2017 ◽  
Vol 8 (3) ◽  
pp. 2199-2203 ◽  
Author(s):  
Hua Li ◽  
Deliang Zhang ◽  
Mengna Gao ◽  
Lumei Huang ◽  
Longguang Tang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Bond Cleavage ◽  
Quantitative Imaging ◽  
Dihydropyridine Derivatives

A novel FRET fluorescence “off–on” system based on the highly specific, sensitive and effective C–C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO).

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