Investigation of the fluorescence quenching behavior of PEI-doped silica nanoparticles and its applications

RSC Advances ◽  
2016 ◽  
Vol 6 (99) ◽  
pp. 97187-97193 ◽  
Author(s):  
Yali Qiao ◽  
Xingwang Zheng
Keyword(s):  
Fluorescence Quenching ◽  
Silica Nanoparticles ◽  
Eosin Y ◽  
Feasible Method ◽  
Quenching Effect ◽  
First Time

A simple and feasible method for overcoming the fluorescence quenching effect of PEI on fluorophores (eosin Y was chosen as the model dye) was designed for the first time.

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

scholarly journals Detection of nitroaromatic vapours with diketopyrrolopyrrole thin films: exploring the role of structural order and morphology on thin film properties and fluorescence quenching efficiency

2015 ◽  
Vol 51 (6) ◽  
pp. 1143-1146 ◽  
Author(s):  
Monika Warzecha ◽  
Jesus Calvo-Castro ◽  
Alan R. Kennedy ◽  
Alisdair N. Macpherson ◽  
Kenneth Shankland ◽  
...  
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Fluorescence Quenching ◽  
Optical Detection ◽  
Film Properties ◽  
Structural Order ◽  
Quenching Efficiency ◽  
First Time

Sensitive optical detection of nitroaromatic vapours with diketopyrrolopyrrole thin films is reported for the first time.

scholarly journals Synthesis of new p-tert-butylcalix[4]arene-based polyammonium triazolyl amphiphiles and their binding with nucleoside phosphates

2018 ◽  
Vol 14 ◽  
pp. 1980-1993 ◽  
Author(s):  
Vladimir A Burilov ◽  
Guzaliya A Fatikhova ◽  
Mariya N Dokuchaeva ◽  
Ramil I Nugmanov ◽  
Diana A Mironova ◽  
...  
Keyword(s):  
Colorimetric Method ◽  
Cycloaddition Reaction ◽  
Water Soluble ◽  
Critical Aggregation Concentration ◽  
Eosin Y ◽  
Replacement Procedure ◽  
Upper Rim ◽  
First Time ◽  
Naked Eye Detection ◽  
Premicellar Aggregates

The synthesis of new calix[4]arenes adopting a cone stereoisomeric form bearing two or four azide fragments on the upper rim and water-soluble triazolyl amphiphilic receptors with two or four polyammonium headgroups via copper-catalyzed azide–alkyne cycloaddition reaction has been performed for the first time. It was found that the synthesized macrocycles form stable aggregates with hydrodynamic diameters between 150–200 nm and electrokinetic potentials about +40 to +60 mV in water solutions. Critical aggregation concentration (CAC) values were measured using a micelle method with pyrene and eosin Y as dye probes. The CAC values of tetraalkyl-substituted macrocycles 12a,b (5 µM for both) are significantly lower than those for dialkyl-substituted macrocycles 10a,b (790 and 160 µM, respectively). Premicellar aggregates of macrocycles 10a,b and 12a,b with the dye eosin Y were used for nucleotides sensing through a dye replacement procedure. It is unusual that disubstituted macrocycles 10a,b bind more effectively a less charged adenosine 5'-diphosphate (ADP) than adenosine 5'-triphosphate (ATP). A simple colorimetric method based on polydiacetylene vesicles decorated with 10b was elaborated for the naked-eye detection of ADP with a detection limit of 0.5 mM.

scholarly journals Investigation of 1-Methylcytosine as a Ligand in Gold(III) Complexes: Synthesis and Protein Interactions

Inorganics ◽  
2018 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
James Beaton ◽  
Nicholas Farrell
Keyword(s):  
Fluorescence Quenching ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Nucleocapsid Protein ◽  
1H Nmr Spectroscopy ◽  
Ionization Mass ◽  
Target Interaction ◽  
Self Association ◽  
A Charge ◽  
First Time

The HIV nucleocapsid protein NCp7 was previously shown to play a number of roles in the viral life cycle and was previously identified as a potential target for small molecule intervention. In this work, the synthesis of the previously unreported complexes [Au(dien)(1MeCyt)]3+, [Au(N-Medien)(1MeCyt)]3+, and [Au(dien)(Cyt)]3+ is detailed, and the interactions of these complexes with the models for NCp7 are described. The affinity for these complexes with the target interaction site, the “essential” tryptophan of the C-terminal zinc finger motif of NCp7, was investigated through the use of a fluorescence quenching assay and by 1H-NMR spectroscopy. The association of [Au(dien)(1MeCyt)]3+ as determined through fluorescence quenching is intermediate between the previously reported DMAP and 9-EtGua analogs, while the associations of [Au(N-Medien)(1MeCyt)]3+ and [Au(dien)(Cyt)]3+ are lower than the previously reported complexes. Additionally, NMR investigation shows that the self-association of relevant compounds is negligible. The specifics of the interaction with the C-terminal zinc finger were investigated by circular dichroism spectroscopy and electrospray-ionization mass spectrometry. The interaction is complete nearly immediately upon mixing, and the formation of AuxFn+ (x = 1, 2, or 4; F = apopeptide) concomitant with the loss of all ligands is observed. Additionally, oxidized dimerized peptide was observed for the first time as a product, indicating a reaction via a charge transfer mechanism.

Spectroscopic and molecular modeling studies of human serum albumin interaction with propyl gallate

RSC Advances ◽  
2014 ◽  
Vol 4 (110) ◽  
pp. 64559-64564 ◽  
Author(s):  
Jafar Ezzati Nazhad Dolatabadi ◽  
Vahid Panahi-Azar ◽  
Abolfazl Barzegar ◽  
Ali Akbar Jamali ◽  
Fahimeh Kheirdoosh ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Human Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Human Serum ◽  
Propyl Gallate ◽  
Fluorescence Quenching Method ◽  
Molecular Modeling Studies ◽  
Quenching Method ◽  
First Time

For the first time, PG interaction with HSA using fluorescence quenching method, circular dichroism spectroscopy and molecular modeling was investigated.

Properties of the EL2 Level in Organometallic Ga1−xAlxAs

1987 ◽  
Vol 104 ◽  
Author(s):  
A. Ben Cherifa ◽  
R. Azoulay ◽  
G. Guillot
Keyword(s):  
Vapor Deposition ◽  
Deep Level Transient Spectroscopy ◽  
Metalorganic Chemical Vapor Deposition ◽  
Deep Level ◽  
Chemical Vapor ◽  
Electron Trap ◽  
Quenching Effect ◽  
Deep Electron Trap ◽  
Transient Spectroscopy ◽  
First Time

ABSTRACTWe have studied by means of deep level transient spectroscopy and photocapacitance measurements deep electron traps in undoped Ga1−xAlxAs of n-type grown by metalorganic chemical vapor deposition with 0≤x≤ 0.3. A dominant deep electron trap is detected in the series of alloys. Its activation energy is found at EC-0.8 eV in GaAs and it increases with x. Its concentration is found nearly independent of x. For the first time we observed for this level in the Ga1−xAlxAs alloys, the photocapacitance quenching effect typical for the EL2 defect in GaAs thus confirming clearly that EL2 is also created in MOCVD Ga1−xAlxAs.

Black phosphorus nanosheets for rapid microRNA detection

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5060-5064 ◽  
Author(s):  
Jie Zhou ◽  
Zhongjun Li ◽  
Ming Ying ◽  
Maixian Liu ◽  
Xiaomei Wang ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Rapid Detection ◽  
Black Phosphorus ◽  
Sensing Platform ◽  
Microrna Detection ◽  
First Time

Herein, for the first time, a sensitive sensing platform for rapid detection of microRNA was developed by employing black phosphorus nanosheets as the fluorescence quenching material.

A simple enzyme-catalyzed reaction induced “switch” type fluorescence biosensor based on carbon nitride nanosheets for the assay of alkaline phosphatase activity

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6277-6282
Author(s):  
Xi Zhu ◽  
Huifeng Xu ◽  
Yan Zhan ◽  
Wenjing Li ◽  
Yongqiang Dong ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fluorescence Quenching ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Carbon Nitride ◽  
Quenching Effect ◽  
Fluorescence Switch ◽  
Switch Type ◽  
Fluorescence Biosensor ◽  
Carbon Nitride Nanosheets

An enzyme-catalyzed fluorescence “switch” type sensor was constructed for assay of alkaline phosphatase activity by combining the fluorescence quenching effect of Ag+ on ultrathin g-C3N4 nanosheets with the simple redox reaction of AA and Ag+.

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