Preferential binding of anticancer drugs to triplex DNA compared to duplex DNA: a spectroscopic and calorimetric study

RSC Advances ◽  
2016 ◽  
Vol 6 (46) ◽  
pp. 39903-39917 ◽  
Author(s):  
Neelam Lohani ◽  
Moganty R. Rajeswari
Keyword(s):  
Anticancer Drugs ◽  
Promoter Region ◽  
Binding Study ◽  
Triplex Dna ◽  
Calorimetric Study ◽  
Duplex Dna ◽  
Calorimetric Technique ◽  
Preferential Binding

Binding study of adriamycin and actinomycin to triplex DNA formed on the promoter region of hmgb1 gene using spectroscopic and calorimetric technique.

scholarly journals Evaluation of the stoichiometry and energetics of carbohydrate binding to Ricinus communis agglutinin: a calorimetric study

1998 ◽  
Vol 333 (3) ◽  
pp. 539-542 ◽  
Author(s):  
Shalini SHARMA ◽  
Satish BHARADWAJ ◽  
Avadhesha SUROLIA ◽  
Sunil Kumar PODDER
Keyword(s):  
Ricinus Communis ◽  
Binding Constants ◽  
High Sensitivity ◽  
Titration Calorimetry ◽  
Water Molecules ◽  
Carbohydrate Binding ◽  
Calorimetric Study ◽  
Ricinus Communis Agglutinin ◽  
Preferential Binding ◽  
Site Binding

High-sensitivity isothermal titration calorimetry has been used to investigate the thermodynamics of binding of Ricinus communis agglutinin to galactose, lactose and their derivatives in the temperature range 280.5–298 K. The present study unequivocally establishes the carbohydrate-binding stoichiometry of the tetrameric agglutinin from castor bean as two, i.e. the (As–sB)2-type tetramer of the agglutinin has two equivalent sites that are non-interacting and independent. The site binding constants range from 2.2×103 M-1 at 282 K for galactose to 4.84×104 M-1 at 281 K for N-acetyl-lactosamine. The binding enthalpies range from -21.9 kJ·mol-1 at 293 K for 4-methylumbelliferyl-β-galactoside to -50.2 kJ·mol-1 at 292.9 K for thiodigalactoside. The observation of limited entropy–enthalpy compensation for binding of the sugars to the lectin indicates that reorganization of water molecules plays an important role in binding. As the slope of the compensation plot is greater than unity, the reactions are largely enthalpically driven. These studies show that the stronger binding of N-acetyl-lactosamine than lactose is due to a favourable interaction between the acetamido group of the reducing-end N-acetylglucosamine of the former and the corresponding loci in the agglutinin molecule. Preferential binding of methyl-β-galactoside over methyl-α-galactoside also indicates the apolar nature of the interaction with the methyl group of the former sugar.

A Molecular Beacon Strategy for the Thermodynamic Characterization of Triplex DNA:  Triplex Formation at the Promoter Region of Cyclin D1†

Biochemistry ◽  
2001 ◽  
Vol 40 (31) ◽  
pp. 9387-9395 ◽  
Author(s):  
Thomas Antony ◽  
Thresia Thomas ◽  
Leonard H. Sigal ◽  
Akira Shirahata ◽  
T. J. Thomas
Keyword(s):  
Cyclin D1 ◽  
Promoter Region ◽  
Molecular Beacon ◽  
Triplex Dna ◽  
Thermodynamic Characterization ◽  
Triplex Formation ◽  
Dna Triplex

Preferential Binding of 3,3‘-Diethyloxadicarbocyanine to Triplex DNA

2000 ◽  
Vol 122 (2) ◽  
pp. 424-425 ◽  
Author(s):  
Jinsong Ren ◽  
Jonathan B. Chaires
Keyword(s):  
Triplex Dna ◽  
Preferential Binding

Groove Binding Ligands for the Interaction with Parallel-Strandedps-Duplex DNA and Triplex DNA

2010 ◽  
Vol 21 (8) ◽  
pp. 1389-1403 ◽  
Author(s):  
Akash K. Jain ◽  
Santanu Bhattacharya
Keyword(s):  
Triplex Dna ◽  
Duplex Dna ◽  
Groove Binding

scholarly journals Quadruplex formation is necessary for stable PNA invasion into duplex DNA of BCL2 promoter region

2011 ◽  
Vol 39 (16) ◽  
pp. 7114-7123 ◽  
Author(s):  
M. I. Onyshchenko ◽  
T. I. Gaynutdinov ◽  
E. A. Englund ◽  
D. H. Appella ◽  
R. D. Neumann ◽  
...  
Keyword(s):  
Promoter Region ◽  
Duplex Dna ◽  
Quadruplex Formation

scholarly journals Transcription factors as targets of anticancer drugs.

1999 ◽  
Vol 46 (2) ◽  
pp. 255-262 ◽  
Author(s):  
M Gniazdowski ◽  
M Czyz
Keyword(s):  
Transcription Factors ◽  
Anticancer Drugs ◽  
Binding Sites ◽  
Promoter Region ◽  
Alkylating Agents ◽  
New Drugs ◽  
Pharmacological Properties ◽  
Specific Interactions ◽  
Covalent Modifications ◽  
Specific Sequences

Several general and gene- and cell-selective transcription factors are required for specific transcription to occur. Many of them exert their functions through specific contacts either in the promoter region or at distant sequences regulating the initiation. These contacts may be altered by anticancer drugs which form non-covalent complexes with DNA. Covalent modifications of DNA by alkylating agents may prevent transcription factors from recognizing their specific sequences or may constitute multiple "unnatural" binding sites in DNA which attract the factors thus decreasing their availability in the cell. The anticancer drug-transcription factor interplay which is based on specific interactions with DNA may contribute to pharmacological properties of the former and provide a basis for the search for new drugs.

scholarly journals Selectivity of a bromoacridine-containing fluorophore for triplex DNA

2021 ◽  
Author(s):  
Sanjeev Kumar Sharma ◽  
William Fraser
Keyword(s):  
Dna Sequences ◽  
Bromine Atom ◽  
Triplex Dna ◽  
Target Sequence ◽  
Duplex Dna ◽  
Synthetic Route

AbstractFluorophore 1,8-naphthilamide was linked to 2-bromoacridine through an ethylenediamine spacer using a succinct synthetic route to give a bromoacridine-linked, bifunctional fluorophore conjugate for the detection of triplex DNA. Acridine is well known to intercalate into duplex DNA whereas introduction of a bulky bromine atom at position C2 redirects specificity for triplex over duplex DNA. In this work, photoelectron transfer assay was used to demonstrate that the synthesised 2-bromoacridine-linked fluorophore conjugate had good selectivity for the representative triplex DNA target sequence d(T*A.T)20 compared with double-stranded d(T.A)20, single-stranded dT20 or d(G/A)19 DNA sequences. Graphic abstract

A Calorimetric Study on the Precipitation of T1 Phase during the Ageing of an Al-Li-Cu-Zr Alloy

2008 ◽  
Vol 273-276 ◽  
pp. 14-17 ◽  
Author(s):  
Shahram Ahmadi ◽  
Ali Shokuhfar ◽  
M.R. Abotalebi ◽  
Arash Rezaei
Keyword(s):  
Activation Energies ◽  
Alloy Sheet ◽  
Differential Scanning Calorimetric ◽  
Calorimetric Study ◽  
Temperature Range ◽  
Calorimetric Technique ◽  
Zr Alloy

The precipitation of T1 phase during the ageing of an Al- Li- Cu -Zr alloy sheet was studied by DSC (Differential Scanning Calorimetric) technique. Results showed that precipitation of T1 phase occurred in temperature range of 250°C to 300°C whereas its dissolution occurred within the temperature of 450°C to 530°C. Furthermore, activation energies for precipitation and dissolution of T1 phase were determined 122.1 kJ/mol and 130.3 kJ/mol, respectively.

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