Fluorescent C-dot nanocomposites as efficient photothermal agents and multi-modal imaging tracers

2017 ◽  
Vol 1 (3) ◽  
pp. 538-541 ◽  
Author(s):  
Zhe Liu ◽  
Qien Xu ◽  
Yihong Li ◽  
Waner Chen
Keyword(s):  
Blood Vessels ◽  
Cell Tracking ◽  
Photoacoustic Imaging

Fluorescent C-dot nanocomposites were developed as photothermal agents and multi-modal tracers for cell tracking and photoacoustic imaging of ocular blood vessels.

scholarly journals Assessment of Brain Functional Activity Using a Miniaturized Head-Mounted Scanning Photoacoustic Imaging System in Awake and Freely Moving Rats

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 429
Author(s):  
Yuhling Wang ◽  
Tsung-Sheng Chu ◽  
Yan-Ren Lin ◽  
Chia-Hui Tsao ◽  
Chia-Hua Tsai ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
T Test ◽  
Natural Behavior ◽  
Freely Moving Rats ◽  
Anesthetized Rats ◽  
Freely Moving ◽  
Paired T Test ◽  
Awake Rats

Understanding the relationship between brain function and natural behavior remains a significant challenge in neuroscience because there are very few convincing imaging/recording tools available for the evaluation of awake and freely moving animals. Here, we employed a miniaturized head-mounted scanning photoacoustic imaging (hmPAI) system to image real-time cortical dynamics. A compact photoacoustic (PA) probe based on four in-house optical fiber pads and a single custom-made 48-MHz focused ultrasound transducer was designed to enable focused dark-field PA imaging, and miniature linear motors were included to enable two-dimensional (2D) scanning. The total dimensions and weight of the proposed hmPAI system are only approximately 50 × 64 × 48 mm and 58.7 g (excluding cables). Our ex vivo phantom experimental tests revealed that a spatial resolution of approximately 0.225 mm could be achieved at a depth of 9 mm. Our in vivo results further revealed that the diameters of cortical vessels draining into the superior sagittal sinus (SSS) could be clearly imaged and continuously observed in both anesthetized rats and awake, freely moving rats. Statistical analysis showed that the full width at half maximum (FWHM) of the PA A-line signals (relative to the blood vessel diameter) was significantly increased in the selected SSS-drained cortical vessels of awake rats (0.58 ± 0.17 mm) compared with those of anesthetized rats (0.31 ± 0.09 mm) (p < 0.01, paired t-test). In addition, the number of pixels in PA B-scan images (relative to the cerebral blood volume (CBV)) was also significantly increased in the selected SSS-drained blood vessels of awake rats (107.66 ± 23.02 pixels) compared with those of anesthetized rats (81.99 ± 21.52 pixels) (p < 0.01, paired t-test). This outcome may result from a more active brain in awake rats than in anesthetized rats, which caused cerebral blood vessels to transport more blood to meet the increased nutrient demand of the tissue, resulting in an obvious increase in blood vessel volume. This hmPAI system was further validated for utility in the brains of awake and freely moving rats, showing that their natural behavior was unimpaired during vascular imaging, thereby providing novel opportunities for studies of behavior, cognition, and preclinical models of brain diseases.

scholarly journals Photoacoustic imaging of blood vessels with a double-ring sensor featuring a narrow angular aperture

2004 ◽  
Vol 9 (6) ◽  
pp. 1327 ◽  
Author(s):  
Roy G. M. Kolkman ◽  
Erwin Hondebrink ◽  
Wiendelt Steenbergen ◽  
Ton G. van Leeuwen ◽  
Frits F. M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Angular Aperture ◽  
Double Ring

3D-Photoacoustic Imaging of Blood Vessels

1998 ◽  
Author(s):  
C. G. A. Hoelen ◽  
R. Pongers ◽  
A. Dekker ◽  
F. F. M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging

Fast Photoacoustic Imaging of Blood Vessels Based on an Annular Transducer Array

2012 ◽  
Vol 29 (10) ◽  
pp. 104302
Author(s):  
Di-Wu Yang ◽  
Lv-Ming Zeng ◽  
Xuan-Rong Ji ◽  
Zhong Huang ◽  
Xing-Hui Chen ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Transducer Array

scholarly journals In vivo photoacoustic imaging of blood vessels using an extreme-narrow aperture sensor

2003 ◽  
Vol 9 (2) ◽  
pp. 343-346 ◽  
Author(s):  
R.G.M. Kolkman ◽  
E. Hondebrink ◽  
W. Steenbergen ◽  
F.F.M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging

scholarly journals Polymer-Peptide Modified Gold Nanorods to Improve Cell Conjugation and Cell labelling for Stem Cells Photoacoustic Imaging

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1196
Author(s):  
Dina Salah ◽  
Farahat S. Moghanm ◽  
Muhammad Arshad ◽  
Abdulaziz A. Alanazi ◽  
Salman latif ◽  
...  
Keyword(s):  
Stem Cells ◽  
Contrast Agent ◽  
Surface Plasmon ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Cell Tracking ◽  
Photoacoustic Imaging ◽  
Optical Measurements ◽  
Peptide Sequence ◽  
Ammonium Bromide

The use of gold nanorods (GNRs) as a contrast agent in bioimaging and cell tracking has numerous advantages, primarily due to the unique optical properties of gold nanorods which allow for the use of infrared regions when imaging. Owing to their unique geometry, Au NRs exhibit surface plasmon modes in the near-infrared wavelength range, which is ideal for carrying out optical measurements in biological fluids and tissue. Gold nanorod functionalization is essential, since the Cetyltrimethyl ammonium bromide CTAB gold nanorods are toxic, and for further in vitro and in vivo experiments the nanorods should be functionalized to become optically stable and biocompatible. In the present study, gold nanorods with an longitudinal surface plasmon resonance (LSPR) position around 800 nm were synthesized in order to be used for photoacoustic imaging applications for stem cell tracking. The gold nanorods were functionalized using both thiolated poly (ethylene glycol) (PEG) to stabilize the gold nanorods surface and a CALNN–TAT peptide sequence. Both ligands were attached to the gold nanorods through an Au–sulfur bond. CALNN–TAT is known as a cell penetrating peptide which ensures endocytosis of the gold nanorods inside the mesenchymal stem cells of mice (MSCD1). Surface modifications of gold nanorods were achieved using optical spectroscopy (UV–VIS), electron microscopy (TEM), zeta-potential, and FTIR. Gold nanorods were incubated in MSCD1 in order to achieve a cellular uptake that was characterized by a transmission electron microscope (TEM). For photoacoustic imaging, Multi-Spectral Optoacoustic Tomography (MSOT) was used. The results demonstrated good cellular uptake for PEG–CALNN–TAT GNRs and the successful use of modified gold nanorods as both a contrast agent in photoacoustic imaging and as a novel tracking bioimaging technique.

scholarly journals Contrast agents for photoacoustic imaging: a review of stem cell tracking

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Soorya James ◽  
Kai Neuhaus ◽  
Mary Murphy ◽  
Martin Leahy
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Contrast Agents ◽  
Real Time ◽  
Cell Tracking ◽  
Spinal Cord Injuries ◽  
Photoacoustic Imaging ◽  
Heart Diseases ◽  
Biodistribution Studies ◽  
Migration Pathways

AbstractWith the advent of stem cell therapy for spinal cord injuries, stroke, burns, macular degeneration, heart diseases, diabetes, rheumatoid arthritis and osteoarthritis; the need to track the survival, migration pathways, spatial destination and differentiation of transplanted stem cells in a clinical setting has gained increased relevance. Indeed, getting regulatory approval to use these therapies in the clinic depends on biodistribution studies. Although optoacoustic imaging (OAI) or photoacoustic imaging can detect functional information of cell activities in real-time, the selection and application of suitable contrast agents is essential to achieve optimal sensitivity and contrast for sensing at clinically relevant depths and can even provide information about molecular activity. This review explores OAI methodologies in conjunction with the specific application of exogenous contrast agents in comparison to other imaging modalities and describes the properties of exogenous contrast agents for quantitative and qualitative monitoring of stem cells. Specific characteristics such as biocompatibility, the absorption coefficient, and surface functionalization are compared and how the labelling efficiency translates to both short and long-term visualization of mesenchymal stem cells is explored. An overview of novel properties of recently developed optoacoustic contrast agents and their capability to detect disease and recovery progression in clinical settings is provided which includes newly developed exogenous contrast agents to monitor stem cells in real-time for multimodal sensing.

scholarly journals Visualization of tumor-related blood vessels in human breast by photoacoustic imaging system with a hemispherical detector array

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
M. Toi ◽  
Y. Asao ◽  
Y. Matsumoto ◽  
H. Sekiguchi ◽  
A. Yoshikawa ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Detector Array ◽  
Human Breast
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