Simultaneous visualization of nerves and blood vessels with multispectral photoacoustic imaging for intraoperative guidance of neurosurgeries

2019 ◽  
Author(s):  
Michelle Graham ◽  
Joanna Guo ◽  
Muyinatu A. Lediju Bell
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Simultaneous Visualization ◽  
Intraoperative Guidance

scholarly journals Assessment of Brain Functional Activity Using a Miniaturized Head-Mounted Scanning Photoacoustic Imaging System in Awake and Freely Moving Rats

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 429
Author(s):  
Yuhling Wang ◽  
Tsung-Sheng Chu ◽  
Yan-Ren Lin ◽  
Chia-Hui Tsao ◽  
Chia-Hua Tsai ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
T Test ◽  
Natural Behavior ◽  
Freely Moving Rats ◽  
Anesthetized Rats ◽  
Freely Moving ◽  
Paired T Test ◽  
Awake Rats

Understanding the relationship between brain function and natural behavior remains a significant challenge in neuroscience because there are very few convincing imaging/recording tools available for the evaluation of awake and freely moving animals. Here, we employed a miniaturized head-mounted scanning photoacoustic imaging (hmPAI) system to image real-time cortical dynamics. A compact photoacoustic (PA) probe based on four in-house optical fiber pads and a single custom-made 48-MHz focused ultrasound transducer was designed to enable focused dark-field PA imaging, and miniature linear motors were included to enable two-dimensional (2D) scanning. The total dimensions and weight of the proposed hmPAI system are only approximately 50 × 64 × 48 mm and 58.7 g (excluding cables). Our ex vivo phantom experimental tests revealed that a spatial resolution of approximately 0.225 mm could be achieved at a depth of 9 mm. Our in vivo results further revealed that the diameters of cortical vessels draining into the superior sagittal sinus (SSS) could be clearly imaged and continuously observed in both anesthetized rats and awake, freely moving rats. Statistical analysis showed that the full width at half maximum (FWHM) of the PA A-line signals (relative to the blood vessel diameter) was significantly increased in the selected SSS-drained cortical vessels of awake rats (0.58 ± 0.17 mm) compared with those of anesthetized rats (0.31 ± 0.09 mm) (p < 0.01, paired t-test). In addition, the number of pixels in PA B-scan images (relative to the cerebral blood volume (CBV)) was also significantly increased in the selected SSS-drained blood vessels of awake rats (107.66 ± 23.02 pixels) compared with those of anesthetized rats (81.99 ± 21.52 pixels) (p < 0.01, paired t-test). This outcome may result from a more active brain in awake rats than in anesthetized rats, which caused cerebral blood vessels to transport more blood to meet the increased nutrient demand of the tissue, resulting in an obvious increase in blood vessel volume. This hmPAI system was further validated for utility in the brains of awake and freely moving rats, showing that their natural behavior was unimpaired during vascular imaging, thereby providing novel opportunities for studies of behavior, cognition, and preclinical models of brain diseases.

scholarly journals Photoacoustic imaging of blood vessels with a double-ring sensor featuring a narrow angular aperture

2004 ◽  
Vol 9 (6) ◽  
pp. 1327 ◽  
Author(s):  
Roy G. M. Kolkman ◽  
Erwin Hondebrink ◽  
Wiendelt Steenbergen ◽  
Ton G. van Leeuwen ◽  
Frits F. M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Angular Aperture ◽  
Double Ring

3D-Photoacoustic Imaging of Blood Vessels

1998 ◽  
Author(s):  
C. G. A. Hoelen ◽  
R. Pongers ◽  
A. Dekker ◽  
F. F. M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging

Fast Photoacoustic Imaging of Blood Vessels Based on an Annular Transducer Array

2012 ◽  
Vol 29 (10) ◽  
pp. 104302
Author(s):  
Di-Wu Yang ◽  
Lv-Ming Zeng ◽  
Xuan-Rong Ji ◽  
Zhong Huang ◽  
Xing-Hui Chen ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Transducer Array

scholarly journals In vivo photoacoustic imaging of blood vessels using an extreme-narrow aperture sensor

2003 ◽  
Vol 9 (2) ◽  
pp. 343-346 ◽  
Author(s):  
R.G.M. Kolkman ◽  
E. Hondebrink ◽  
W. Steenbergen ◽  
F.F.M. de Mul
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging

Fluorescent C-dot nanocomposites as efficient photothermal agents and multi-modal imaging tracers

2017 ◽  
Vol 1 (3) ◽  
pp. 538-541 ◽  
Author(s):  
Zhe Liu ◽  
Qien Xu ◽  
Yihong Li ◽  
Waner Chen
Keyword(s):  
Blood Vessels ◽  
Cell Tracking ◽  
Photoacoustic Imaging

Fluorescent C-dot nanocomposites were developed as photothermal agents and multi-modal tracers for cell tracking and photoacoustic imaging of ocular blood vessels.

scholarly journals Visualization of tumor-related blood vessels in human breast by photoacoustic imaging system with a hemispherical detector array

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
M. Toi ◽  
Y. Asao ◽  
Y. Matsumoto ◽  
H. Sekiguchi ◽  
A. Yoshikawa ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Detector Array ◽  
Human Breast

Photoacoustic imaging of blood vessels in the chorioallantoic membrane of a chicken embryo

2004 ◽  
Author(s):  
Roy G. M. Kolkman ◽  
Arjan Huisjes ◽  
Ronald I. Siphanto ◽  
Wiendelt Steenbergen ◽  
Ton G. van Leeuwen
Keyword(s):  
Blood Vessels ◽  
Chicken Embryo ◽  
Photoacoustic Imaging ◽  
Chorioallantoic Membrane

scholarly journals In vivo photoacoustic imaging of blood vessels using a homodyne interferometer with zero-crossing triggering

2017 ◽  
Vol 22 (3) ◽  
pp. 036002 ◽  
Author(s):  
Jiao Lu ◽  
Yingzhe Gao ◽  
Zhenhe Ma ◽  
Hongxian Zhou ◽  
Ruikang K. Wang ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
Zero Crossing
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