Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 951-960 ◽  
Author(s):  
Cifeng Fang ◽  
Fujun Ji ◽  
Zhiquan Shu ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Temperature Control ◽  
Temperature Dependent ◽  
Microfluidic Platform ◽  
Instantaneous Flow ◽  
Dependent Cell ◽  
Membrane Permeabilities

We developed an integrated microfluidic platform for instantaneous flow and localized temperature control.

Numerical Modeling of Temperature-Dependent Cell Membrane Permeability to Water Based on a Microfluidic System with Dynamic Temperature Control

2021 ◽  
pp. 247263032110151
Author(s):  
Tianhang Yang ◽  
Ji Peng ◽  
Cifeng Fang ◽  
Songjing Li ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Constant Temperature ◽  
Temperature Control ◽  
Microfluidic System ◽  
Cell Membrane Permeability ◽  
Transfer Model ◽  
Temperature Dependent ◽  
Dynamic Temperature ◽  
Dependent Cell ◽  
On Chip

In order to describe temperature-dependent cell osmotic behaviors in a more reliable method, a novel mathematical mass transfer model coupled with dynamic temperature change has been established based on the combination of a time domain to temperature domain transformation equation and a constant temperature mass transfer model. This novel model is numerically simulated under multiple temperature changing rates and extracellular osmolarities. A microfluidic system that can achieve single-cell osmotic behavior observation and provide dynamic and swift on-chip temperature control was built and tested in this paper. Utilizing the temperature control system, the on-chip heating processes are recorded and then described as polynomial time–temperature relationships. These dynamic temperature changing profiles were performed by obtaining cell membrane properties by parameter fitting only one set of testing experimental data to the mathematical model with a constant temperature changing rate. The numerical modeling results show that predicting the osmotic cell volume change using selected dynamic temperature profiles is more suitable for studies concerning cell membrane permeability determination and cryopreservation process than tests using constant temperature changing rates.

Integrated microfluidic platform for instantaneous flow and localized temperature control

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85620-85629 ◽  
Author(s):  
Cifeng Fang ◽  
Doojin Lee ◽  
Boris Stober ◽  
Gerald G. Fuller ◽  
Amy Q. Shen
Keyword(s):  
Temperature Control ◽  
Microfluidic Platform ◽  
Instantaneous Flow

We developed an integrated microfluidic platform for instantaneous flow and localized temperature control.

Fluorescence imaging of cholesterol and temperature dependent cell membrane dynamics

2007 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Wolfgang S. L. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Fluorescence Imaging ◽  
Membrane Dynamics ◽  
Temperature Dependent ◽  
Dependent Cell

scholarly journals Temperature Dependence of G and D’ Phonons in Monolayer to Few-Layer Graphene with Vacancies

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Mingming Yang ◽  
Longlong Wang ◽  
Xiaofen Qiao ◽  
Yi Liu ◽  
Yufan Liu ◽  
...  
Keyword(s):  
Negative Temperature Coefficient ◽  
Negative Temperature ◽  
Intrinsic Properties ◽  
Temperature Dependent ◽  
Phonon Interaction ◽  
Electron Phonon Interaction ◽  
Layer Graphene ◽  
A Value ◽  
Few Layer Graphene

Abstract The defects into the hexagonal network of a sp2-hybridized carbon atom have been demonstrated to have a significant influence on intrinsic properties of graphene systems. In this paper, we presented a study of temperature-dependent Raman spectra of G peak and D’ band at low temperatures from 78 to 318 K in defective monolayer to few-layer graphene induced by ion C+ bombardment under the determination of vacancy uniformity. Defects lead to the increase of the negative temperature coefficient of G peak, with a value almost identical to that of D’ band. However, the variation of frequency and linewidth of G peak with layer number is contrary to D’ band. It derives from the related electron-phonon interaction in G and D’ phonon in the disorder-induced Raman scattering process. Our results are helpful to understand the mechanism of temperature-dependent phonons in graphene-based materials and provide valuable information on thermal properties of defects for the application of graphene-based devices.

scholarly journals Selectivity of Tungsten Oxide Synthesized by Sol-Gel Method Towards Some Volatile Organic Compounds and Gaseous Materials in a Broad Range of Temperatures

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 523 ◽  
Author(s):  
Simonas Ramanavičius ◽  
Milda Petrulevičienė ◽  
Jurga Juodkazytė ◽  
Asta Grigucevičienė ◽  
Arūnas Ramanavičius
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Sol Gel ◽  
Temperature Dependent ◽  
Sensing Systems ◽  
Sensing Material ◽  
Operational Temperature ◽  
Volatile Organic ◽  
Different Gases

In this research, the investigation of sensing properties of non-stoichiometric WO3 (WO3−x) film towards some volatile organic compounds (VOC) (namely: Methanol, ethanol, isopropanol, acetone) and ammonia gas are reported. Sensors were tested at several temperatures within the interval ranging from a relatively low temperature of 60 up to 270 °C. Significant variation of selectivity, which depended on the operational temperature of sensor, was observed. Here, the reported WO3/WO3–x-based sensing material opens an avenue for the design of sensors with temperature-dependent sensitivity, which can be applied in the design of new gas- and/or VOC-sensing systems that are dedicated for the determination of particular gas- and/or VOC-based analyte concentration in the mixture of different gases and/or VOCs, using multivariate analysis of variance (MANOVA).

ArabidopsisHSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses

2014 ◽  
Vol 202 (4) ◽  
pp. 1320-1334 ◽  
Author(s):  
Fei Bao ◽  
Xiaozhen Huang ◽  
Chipan Zhu ◽  
Xiaoyan Zhang ◽  
Xin Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Defense Responses ◽  
Temperature Dependent ◽  
Dependent Cell
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