Fluorescence imaging of cholesterol and temperature dependent cell membrane dynamics

2007 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Wolfgang S. L. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Fluorescence Imaging ◽  
Membrane Dynamics ◽  
Temperature Dependent ◽  
Dependent Cell

Numerical Modeling of Temperature-Dependent Cell Membrane Permeability to Water Based on a Microfluidic System with Dynamic Temperature Control

2021 ◽  
pp. 247263032110151
Author(s):  
Tianhang Yang ◽  
Ji Peng ◽  
Cifeng Fang ◽  
Songjing Li ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Constant Temperature ◽  
Temperature Control ◽  
Microfluidic System ◽  
Cell Membrane Permeability ◽  
Transfer Model ◽  
Temperature Dependent ◽  
Dynamic Temperature ◽  
Dependent Cell ◽  
On Chip

In order to describe temperature-dependent cell osmotic behaviors in a more reliable method, a novel mathematical mass transfer model coupled with dynamic temperature change has been established based on the combination of a time domain to temperature domain transformation equation and a constant temperature mass transfer model. This novel model is numerically simulated under multiple temperature changing rates and extracellular osmolarities. A microfluidic system that can achieve single-cell osmotic behavior observation and provide dynamic and swift on-chip temperature control was built and tested in this paper. Utilizing the temperature control system, the on-chip heating processes are recorded and then described as polynomial time–temperature relationships. These dynamic temperature changing profiles were performed by obtaining cell membrane properties by parameter fitting only one set of testing experimental data to the mathematical model with a constant temperature changing rate. The numerical modeling results show that predicting the osmotic cell volume change using selected dynamic temperature profiles is more suitable for studies concerning cell membrane permeability determination and cryopreservation process than tests using constant temperature changing rates.

Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 951-960 ◽  
Author(s):  
Cifeng Fang ◽  
Fujun Ji ◽  
Zhiquan Shu ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Temperature Control ◽  
Temperature Dependent ◽  
Microfluidic Platform ◽  
Instantaneous Flow ◽  
Dependent Cell ◽  
Membrane Permeabilities

We developed an integrated microfluidic platform for instantaneous flow and localized temperature control.

scholarly journals Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies

2007 ◽  
Vol 179 (5) ◽  
pp. 1067-1082 ◽  
Author(s):  
Valeria R. Caiolfa ◽  
Moreno Zamai ◽  
Gabriele Malengo ◽  
Annapaola Andolfo ◽  
Chris D. Madsen ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Plasminogen Activator ◽  
Fluorescent Protein ◽  
Surface Protein ◽  
Basal Membrane ◽  
Membrane Dynamics ◽  
Live Cells ◽  
Cell Surface Protein ◽  
Protein Assemblies

To search for functional links between glycosylphosphatidylinositol (GPI) protein monomer–oligomer exchange and membrane dynamics and confinement, we studied urokinase plasminogen activator (uPA) receptor (uPAR), a GPI receptor involved in the regulation of cell adhesion, migration, and proliferation. Using a functionally active fluorescent protein–uPAR in live cells, we analyzed the effect that extracellular matrix proteins and uPAR ligands have on uPAR dynamics and dimerization at the cell membrane. Vitronectin directs the recruitment of dimers and slows down the diffusion of the receptors at the basal membrane. The commitment to uPA–plasminogen activator inhibitor type 1–mediated endocytosis and recycling modifies uPAR diffusion and induces an exchange between uPAR monomers and dimers. This exchange is fully reversible. The data demonstrate that cell surface protein assemblies are important in regulating the dynamics and localization of uPAR at the cell membrane and the exchange of monomers and dimers. These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents.

ArabidopsisHSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses

2014 ◽  
Vol 202 (4) ◽  
pp. 1320-1334 ◽  
Author(s):  
Fei Bao ◽  
Xiaozhen Huang ◽  
Chipan Zhu ◽  
Xiaoyan Zhang ◽  
Xin Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Defense Responses ◽  
Temperature Dependent ◽  
Dependent Cell

PMR4-dependent cell wall depositions are a consequence but not the cause of temperature-induced autoimmunity

2021 ◽  
Author(s):  
Giuliana Hessler ◽  
Stephan Michael Portheine ◽  
Eva-Maria Gerlach ◽  
Tim Lienemann ◽  
Gerald Koch ◽  
...  
Keyword(s):  
Cell Wall ◽  
Immune System ◽  
Severe Damage ◽  
Temperature Dependent ◽  
Callose Synthase ◽  
Ambient Temperatures ◽  
Point Of No Return ◽  
Dependent Cell ◽  
And Function ◽  
Reduced Temperature

Abstract Plants possess a well-balanced immune system that is required for defense against pathogen infections. In autoimmune mutants or necrotic crosses, an intrinsic temperature-dependent imbalance leads to constitutive immune activation, resulting in severe damage or even death of plants. Recently, cell wall depositions were described as one of the symptoms following induction of the autoimmune phenotype in Arabidopsis saul1-1 mutants. However, the regulation and function of these depositions remained unclear. Here, we show that cell wall depositions, containing lignin and callose, were a common autoimmune feature and were deposited in proportion to the severity of the autoimmune phenotype at reduced ambient temperatures. When plants were exposed to reduced temperature for periods insufficient to induce an autoimmune phenotype, the cell wall depositions were not present. After low temperature intervals, sufficient to induce autoimmune responses, cell wall depositions correlated with a point of no return in saul1-1 autoimmunity. Although cell wall depositions were largely abolished in saul1-1 pmr4-1 double mutants lacking SAUL1 and the callose synthase gene GSL5/PMR4, their phenotype remained unchanged compared to that of the saul1-1 single mutant. Our data showed that cell wall depositions generally occur in autoimmunity, but appear not to be the cause of autoimmune phenotypes.

scholarly journals Application of Spot Variation FCS (svFCS) Analysis to T Cell Membrane Dynamics

2020 ◽  
Vol 118 (3) ◽  
pp. 353a
Author(s):  
Yannick Hamon ◽  
Anne-Marie Sartre ◽  
Anthony Formisano ◽  
Sébastien Mailfert ◽  
Didier Marguet ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Membrane ◽  
Membrane Dynamics ◽  
T Cell Membrane
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