scholarly journals Imaging and therapeutic applications of zinc(ii)-dipicolylamine molecular probes for anionic biomembranes

2016 ◽  
Vol 52 (57) ◽  
pp. 8787-8801 ◽  
Author(s):  
Douglas R. Rice ◽  
Kasey J. Clear ◽  
Bradley D. Smith
Keyword(s):  
Molecular Imaging ◽  
Molecular Probes ◽  
Targeted Therapeutics ◽  
Therapeutic Applications

Synthetic ZnDPA receptors are used for molecular imaging of disease and targeted therapeutics.

scholarly journals In Vitro Evaluation of Radiolabeled Amphotericin B for Molecular Imaging of Mold Infections

2020 ◽  
Vol 64 (7) ◽  
Author(s):  
Lukas Page ◽  
Andrew J. Ullmann ◽  
Fabian Schadt ◽  
Sebastian Wurster ◽  
Samuel Samnick
Keyword(s):  
Molecular Imaging ◽  
Amphotericin B ◽  
Invasive Pulmonary Aspergillosis ◽  
Nuclear Imaging ◽  
Molecular Probes ◽  
Content Type ◽  
Diagnostic Potential ◽  
Gallium 68

ABSTRACT Invasive pulmonary aspergillosis and mucormycosis are life-threatening complications in immunocompromised patients. A rapid diagnosis followed by early antifungal treatment is essential for patient survival. Given the limited spectrum of biomarkers for invasive mold infections, recent studies have proposed the use of radiolabeled siderophores or antibodies as molecular probes to increase the specificity of radiological findings by nuclear imaging modalities. While holding enormous diagnostic potential, most of the currently available molecular probes are tailored to the detection of Aspergillus species, and their cost-intensive and sophisticated implementation restricts their accessibility at less specialized centers. In order to develop cost-efficient and broadly applicable tracers for pulmonary mold infections, this study established streamlined and high-yielding protocols to radiolabel amphotericin B (AMB) with the gamma emitter technetium-99m (99mTc-AMB) and the positron emitter gallium-68 (68Ga-AMB). The radiochemical purity of the resulting tracers consistently exceeded 99%, and both probes displayed excellent stability in human serum (>98% after 60 to 240 min at 37°C). The uptake kinetics by representative mold pathogens were assessed in an in vitro Transwell assay using infected endothelial cell layers. Both tracers accumulated intensively and specifically in Transwell inserts infected with Aspergillus fumigatus, Rhizopus arrhizus, and other clinically relevant mold pathogens compared with their accumulation in uninfected inserts and inserts infected with bacterial controls. Inoculum-dependent enrichment was confirmed by gamma counting and autoradiographic imaging. Taken together, this pilot in vitro study proposes 99mTc-AMB and 68Ga-AMB to be facile, stable, and specific probes, meriting further preclinical in vivo evaluation of radiolabeled amphotericin B for molecular imaging in invasive mycoses.

Synthesis of fluorescent molecular probes based on cis-cinnamic acid and molecular imaging of lettuce roots

Tetrahedron ◽  
2016 ◽  
Vol 72 (41) ◽  
pp. 6492-6498 ◽  
Author(s):  
Hiroshi Fukuda ◽  
Keisuke Nishikawa ◽  
Yukihiro Fukunaga ◽  
Katsuhiro Okuda ◽  
Kozue Kodama ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Cinnamic Acid ◽  
Molecular Probes ◽  
Fluorescent Molecular Probes

scholarly journals Chemical probes for molecular imaging and detection of hydrogen sulfide and reactive sulfur species in biological systems

2015 ◽  
Vol 44 (14) ◽  
pp. 4596-4618 ◽  
Author(s):  
Vivian S. Lin ◽  
Wei Chen ◽  
Ming Xian ◽  
Christopher J. Chang
Keyword(s):  
Hydrogen Sulfide ◽  
Molecular Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Biological Systems ◽  
Molecular Probes ◽  
Chemical Probes ◽  
Sulfur Species ◽  
Sulfane Sulfur ◽  
Reactive Sulfur Species

This review highlights progress in the development of molecular probes for live cell imaging of hydrogen sulfide and other reactive sulfur species, including sulfite, bisulfite, sulfane sulfur species, and S-nitrosothiols.

scholarly journals Identify. Quantify. Predict. Why Immunologists Should Widely Use Molecular Imaging for Coronavirus Disease 2019

2021 ◽  
Vol 12 ◽  
Author(s):  
Freimut D. Juengling ◽  
Antonio Maldonado ◽  
Frank Wuest ◽  
Thomas H. Schindler
Keyword(s):  
Molecular Imaging ◽  
Current Knowledge ◽  
Imaging Modality ◽  
Acute Infection ◽  
Molecular Probes ◽  
Whole Body ◽  
Prognostic Indicators ◽  
Imaging Biomarkers ◽  
Body Scale

Molecular imaging using PET/CT or PET/MRI has evolved from an experimental imaging modality at its inception in 1972 to an integral component of diagnostic procedures in oncology, and, to lesser extent, in cardiology and neurology, by successfully offering in-vivo imaging and quantitation of key pathophysiological targets or molecular signatures, such as glucose metabolism in cancerous disease. Apart from metabolism probes, novel radiolabeled peptide and antibody PET tracers, including radiolabeled monoclonal antibodies (mAbs) have entered the clinical arena, providing the in-vivo capability to collect target-specific quantitative in-vivo data on cellular and molecular pathomechanisms on a whole-body scale, and eventually, extract imaging biomarkers possibly serving as prognostic indicators. The success of molecular imaging in mapping disease severity on a whole-body scale, and directing targeted therapies in oncology possibly could translate to the management of Coronavirus Disease 2019 (COVID-19), by identifying, localizing, and quantifying involvement of different immune mediated responses to the infection with SARS-COV2 during the course of acute infection and possible, chronic courses with long-term effects on specific organs. The authors summarize current knowledge for medical imaging in COVID-19 in general with a focus on molecular imaging technology and provide a perspective for immunologists interested in molecular imaging research using validated and immediately available molecular probes, as well as possible future targets, highlighting key targets for tailored treatment approaches as brought up by key opinion leaders.

scholarly journals Mechanistic and high-throughput approaches for the design of molecular imaging probes and targeted therapeutics

2014 ◽  
Vol 2 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Uwe Haberkorn ◽  
Walter Mier ◽  
Antonia Dimitrakopoulou-Strauss ◽  
Matthias Eder ◽  
Klaus Kopka ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
High Throughput ◽  
Targeted Therapeutics ◽  
Molecular Imaging Probes ◽  
Imaging Probes

scholarly journals Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET)

2007 ◽  
Vol 50 (3) ◽  
pp. 445-459 ◽  
Author(s):  
Leonard I. Wiebe
Keyword(s):  
Positron Emission Tomography ◽  
Molecular Imaging ◽  
Molecular Biology ◽  
Nucleic Acids ◽  
Therapy Monitoring ◽  
Molecular Probes ◽  
The Body ◽  
New Drugs ◽  
Emission Tomography ◽  
Positron Emission

Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology. Originally, molecular biology meant the biology of gene expression, but now molecular biology broadly encompasses the macromolecular biology and biochemistry of proteins, complex carbohydrates and nucleic acids. To date, molecular imaging has focused primarily on proteins, with emphasis on monoclonal antibodies and their derivative forms, small-molecule enzyme substrates and components of cell membranes, including transporters and transmembrane signalling elements. This overview provides an introduction to nucleosides, nucleotides and nucleic acids in the context of molecular imaging.

POLYMER-MODIFIED GADOLINIUM NANOPARTICLES FOR TARGETED MAGNETIC RESONANCE IMAGING AND THERAPY

Nano LIFE ◽  
2010 ◽  
Vol 01 (03n04) ◽  
pp. 263-275 ◽  
Author(s):  
STEPHEN G. BOYES ◽  
MISTY D. ROWE ◽  
NATALIE J. SERKOVA ◽  
FERNANDO J. KIM ◽  
JAMES R. LAMBERT ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Molecular Probes ◽  
Imaging Agents ◽  
Resonance Imaging ◽  
Non Invasive ◽  
Gene And Protein Expression ◽  
Wide Range

Functional imaging is a novel area in radiological sciences and allows for the non-invasive assessment and visualization of specific targets such as gene and protein expression, metabolic rates, and drug delivery in intact living subjects. As such, the field of molecular imaging has been defined as the non-invasive, quantitative, and repetitive imaging of biomolecules and biological processes in living organisms. For example, cancer cells may be genetically altered to attract molecules that alter the magnetic susceptibility, thereby permitting their identification by magnetic resonance imaging. These contrast agents and/or molecular reporters are seen as essential to the task of molecular medicine to increase both sensitivity and specificity of imaging. Therefore, there are five general principles which need to be fulfilled in order to conduct a successful in vivo molecular imaging study: (1) selection of appropriate cellular and subcellular targets; (2) development of suitable in vivo affinity ligands (molecular probes); (3) delivery of these probes to the target organ; (4) amplification strategies able to detect minimal target concentrations; and (5) development of imaging systems with high resolution. Although there has been a wide range of routes taken to incorporate both imaging agents and a disease-targeting moiety into diagnostic devices, arguably the most interesting of these routes employs the use of nanoparticles. Nanoscale diagnostic systems that incorporate molecular targeting agents and diagnostic imaging capabilities are emerging as the next-generation imaging agents and have the potential to dramatically improve the outcome of the imaging, diagnosis, and treatment of a wide range of diseases. The present review addresses chemical aspects in development of molecular probes based upon gadolinium nanoparticles and their potential role in translational clinical imaging and therapy.

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