scholarly journals A near-infrared AIEgen for specific imaging of lipid droplets

2016 ◽  
Vol 52 (35) ◽  
pp. 5957-5960 ◽  
Author(s):  
Miaomiao Kang ◽  
Xinggui Gu ◽  
Ryan T. K. Kwok ◽  
Chris W. T. Leung ◽  
Jacky W. Y. Lam ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Near Infrared ◽  
High Brightness ◽  
Good Biocompatibility ◽  
Specific Lipid

A new near-infrared AIE luminogen is developed for specific lipid droplet imaging with high brightness, good biocompatibility and superior photostability.

scholarly journals Biogenesis of the multifunctional lipid droplet: Lipids, proteins, and sites

2014 ◽  
Vol 204 (5) ◽  
pp. 635-646 ◽  
Author(s):  
Albert Pol ◽  
Steven P. Gross ◽  
Robert G. Parton
Keyword(s):  
Energy Metabolism ◽  
Lipid Droplet ◽  
Cell Biology ◽  
Lipid Droplets ◽  
Recent Progress ◽  
Human Diseases ◽  
Membrane Synthesis ◽  
Specific Lipid

Lipid droplets (LDs) are ubiquitous dynamic organelles that store and supply lipids in all eukaryotic and some prokaryotic cells for energy metabolism, membrane synthesis, and production of essential lipid-derived molecules. Interest in the organelle’s cell biology has exponentially increased over the last decade due to the link between LDs and prevalent human diseases and the discovery of new and unexpected functions of LDs. As a result, there has been significant recent progress toward understanding where and how LDs are formed, and the specific lipid pathways that coordinate LD biogenesis.

scholarly journals A Novel Photosensitizer for Lipid Droplet–Location Photodynamic Therapy

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiang Xia ◽  
Ran Wang ◽  
Yingqi Hu ◽  
WeiJian Liu ◽  
Ting Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Biological Processes ◽  
Cellular Organelle ◽  
Potential Target ◽  
Ic50 Value ◽  
Good Biocompatibility

Lipid droplets (LDs), an extremely important cellular organelle, are responsible for the storage of neutral lipids in multiple biological processes, which could be a potential target site for photodynamic therapy (PDT) of cancer. Herein, a lipid droplet–targeted photosensitizer (BODSeI) is developed, allowing for fluorescence imaging–guided PDT. Owing to the location of lipid droplets, BODSeI demonstrates enhanced PDT efficiency with an extremely low IC50 value (around 125 nM). Besides, BODSeI shows good biocompatibility and high photostability. Therefore, BODSeI is promising for droplet-location PDT, which may trigger wide interest for exploring the pathway of lipid droplet–location PDT.

scholarly journals Two-photon AIE bio-probe with large Stokes shift for specific imaging of lipid droplets

2017 ◽  
Vol 8 (8) ◽  
pp. 5440-5446 ◽  
Author(s):  
Meijuan Jiang ◽  
Xinggui Gu ◽  
Jacky W. Y. Lam ◽  
Yilin Zhang ◽  
Ryan T. K. Kwok ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Stokes Shift ◽  
Large Stokes Shift ◽  
Two Photon ◽  
Photon Excitation ◽  
Specific Lipid ◽  
Two Photon Excitation ◽  
In Cells

A novel AIEgen with prominent two-photon excitation was rationally developed for specific lipid-droplet imaging in cells and tissues.

Facile construction of boranil complexes with aggregation-induced emission characteristics and their specific lipid droplet imaging applications

2019 ◽  
Vol 55 (58) ◽  
pp. 8494-8497 ◽  
Author(s):  
Na Zhao ◽  
Chengcheng Ma ◽  
Weiyao Yang ◽  
Wei Yin ◽  
Jiahui Wei ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Living Cells ◽  
Emission Characteristics ◽  
Aggregation Induced Emission ◽  
Specific Lipid

A series of boranil complexes with aggregation-induced emission effects were facilely constructed, which can be utilized to image lipid droplets in living cells and yolk lipids in zebrafish.

scholarly journals Indolylbenzothiadiazoles as highly tunable fluorophores for imaging lipid droplet accumulation in astrocytes and glioblastoma cells

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23960-23967
Author(s):  
Kilian Colas ◽  
Karl O. Holmberg ◽  
Linus Chiang ◽  
Susanne Doloczki ◽  
Fredrik J. Swartling ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Glioblastoma Cells ◽  
Photophysical Study

We present an extensive photophysical study of a series of fluorescent indolylbenzothiadiazole derivatives and their ability to specifically image lipid droplets in astrocytes and glioblastoma cells.

scholarly journals Uniform Spheres of α-NaYF4:RE3+ (RE=Eu, Tb, Ce, Er, and Tm): Template-Free Synthesis, Multi-Color Photoluminescence, and Their Application in Cellular Imaging

Crystals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 119
Author(s):  
Xiaofeng Fan ◽  
Laiqin Gu ◽  
Yiling Hu ◽  
Qi Zhu
Keyword(s):  
Near Infrared ◽  
Infrared Emission ◽  
Particle Sizes ◽  
Hydrothermal Processing ◽  
High Brightness ◽  
Luminescent Probes ◽  
Bio Imaging ◽  
Template Free ◽  
Near Infrared Emission

Uniformly dispersed luminescent probes with a high brightness and high resolution are desired in bio imaging fields. Here, ~100 nm sized and well-dispersed spheres of RE3+ doped α-NaYF4 (rare earth (RE) = Eu, Tb, Ce, Er, and Tm) have been facile synthesized through hydrothermal processing in the absence of a template, followed by a proper annealing. The processing window of the cubic structured spheres is wide, because the hydrothermal products are independent of the processing conditions, including reaction time and temperature. The original morphology and crystal structure can be well retained with a calcination temperature up to 600 °C. However, calcination gives rise to a reduction of particle sizes, as a result of the crystallite growth and densification. Under ultraviolet radiation, α-NaYF4:RE3+ spheres show characteristic f-f emissions of RE3+ (RE = Eu, Tb, Ce, Er, and Tm), and exhibit orange red, green, ultraviolet (UV), blue green, and blue emissions, respectively. Mainly because of the near-infrared emission at ~697 nm (5D0→7F4 transitions of Eu3+), the successful imaging of macrophages was achieved by NH2-NaYF4:Eu3+ probes, indicating their excellent imaging capacity for cells in vitro.

Identification of Perilipin-2 as a lipid droplet protein regulated in oocytes during maturation

2010 ◽  
Vol 22 (8) ◽  
pp. 1262 ◽  
Author(s):  
Xing Yang ◽  
Kylie R. Dunning ◽  
Linda L.-Y. Wu ◽  
Theresa E. Hickey ◽  
Robert J. Norman ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Content ◽  
Lipid Droplets ◽  
Intracellular Lipids ◽  
Epidermal Growth ◽  
Novel Method ◽  
Perilipin 2 ◽  
Lipid Droplet Protein

Lipid droplet proteins regulate the storage and utilisation of intracellular lipids. Evidence is emerging that oocyte lipid utilisation impacts embryo development, but lipid droplet proteins have not been studied in oocytes. The aim of the present study was to characterise the size and localisation of lipid droplets in mouse oocytes during the periovulatory period and to identify lipid droplet proteins as potential biomarkers of oocyte lipid content. Oocyte lipid droplets, visualised using a novel method of staining cumulus–oocyte complexes (COCs) with BODIPY 493/503, were small and diffuse in oocytes of preovulatory COCs, but larger and more centrally located after maturation in response to ovulatory human chorionic gonadotrophin (hCG) in vivo, or FSH + epidermal growth factor in vitro. Lipid droplet proteins Perilipin, Perilipin-2, cell death-inducing DNA fragmentation factor 45-like effector (CIDE)-A and CIDE-B were detected in the mouse ovary by immunohistochemistry, but only Perilipin-2 was associated with lipid droplets in the oocyte. In COCs, Perilipin-2 mRNA and protein increased in response to ovulatory hCG. IVM failed to induce Perilipin-2 mRNA, yet oocyte lipid content was increased in this context, indicating that Perilipin-2 is not necessarily reflective of relative oocyte lipid content. Thus, Perilipin-2 is a lipid droplet protein in oocytes and its induction in the COC concurrent with dynamic reorganisation of lipid droplets suggests marked changes in lipid utilisation during oocyte maturation.

scholarly journals HSD17B13: A Potential Therapeutic Target for NAFLD

2022 ◽  
Vol 8 ◽  
Author(s):  
Hai-bo Zhang ◽  
Wen Su ◽  
Hu Xu ◽  
Xiao-yan Zhang ◽  
You-fei Guan
Keyword(s):  
Liver Disease ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
Critical Role ◽  
Chronic Liver ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Nonalcoholic Fatty Liver ◽  
Promising Target ◽  
Associated Proteins

Nonalcoholic fatty liver disease (NAFLD), especially in its inflammatory form (steatohepatitis, NASH), is closely related to the pathogenesis of chronic liver disease. Despite substantial advances in the management of NAFLD/NASH in recent years, there are currently no efficacious therapies for its treatment. The biogenesis and expansion of lipid droplets (LDs) are critical pathophysiological processes in the development of NAFLD/NASH. In the past decade, increasing evidence has demonstrated that lipid droplet-associated proteins may represent potential therapeutic targets for the treatment of NAFLD/NASH given the critical role they play in regulating the biogenesis and metabolism of lipid droplets. Recently, HSD17B13, a newly identified liver-enriched, hepatocyte-specific, lipid droplet-associated protein, has been reported to be strongly associated with the development and progression of NAFLD/NASH in both mice and humans. Notably, human genetic studies have repeatedly reported a robust association of HSD17B13 single nucleotide polymorphisms (SNPs) with the occurrence and severity of NAFLD/NASH and other chronic liver diseases (CLDs). Here we briefly overview the discovery, tissue distribution, and subcellular localization of HSD17B13 and highlight its important role in promoting the pathogenesis of NAFLD/NASH in both experimental animal models and patients. We also discuss the potential of HSD17B13 as a promising target for the development of novel therapeutic agents for NAFLD/NASH.

scholarly journals Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Chuangjia Huang ◽  
Xiaoling Guan ◽  
Hui Lin ◽  
Lu Liang ◽  
Yingling Miao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Good Biocompatibility ◽  
Targeting Ability ◽  
Body Clearance

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

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