Insight into the interactions between nanoparticles and cells

2017 ◽  
Vol 5 (2) ◽  
pp. 173-189 ◽  
Author(s):  
Jingchao Li ◽  
Hongli Mao ◽  
Naoki Kawazoe ◽  
Guoping Chen
Keyword(s):  
Cell Proliferation ◽  
Surface Chemistry ◽  
Cellular Uptake ◽  
Shell Structure ◽  
Protein Corona ◽  
Cellular Responses ◽  
Cellular Mechanics ◽  
Corona Formation ◽  
Structure Surface ◽  
Insight Into

This review summarizes the latest advances in nanoparticle (NP)–cell interactions. The influence of NP size, shape, shell structure, surface chemistry and protein corona formation on cellular uptake and cytotoxicity is highlighted in detail. Their impact on other cellular responses such as cell proliferation, differentiation and cellular mechanics is also discussed.

Protein corona formation and its influence on biomimetic magnetite nanoparticles

2020 ◽  
Vol 8 (22) ◽  
pp. 4870-4882 ◽  
Author(s):  
Ana Peigneux ◽  
Emanuel A. Glitscher ◽  
Rawan Charbaji ◽  
Christoph Weise ◽  
Stefanie Wedepohl ◽  
...  
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Magnetite Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

Colloidal stability and cellular uptake of MamC-biomimetic magnetite nanoparticles (BMNPs) incubated with human plasma (PC-BMNPs).

Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake

Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23259-23267 ◽  
Author(s):  
Alberto Piloni ◽  
Chin Ken Wong ◽  
Fan Chen ◽  
Megan Lord ◽  
Andreas Walther ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Biological Behavior ◽  
Protein Absorption ◽  
Corona Formation

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.

scholarly journals Machine learning provides predictive analysis into silver nanoparticle protein corona formation from physicochemical properties

2018 ◽  
Vol 5 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Matthew R. Findlay ◽  
Daniel N. Freitas ◽  
Maryam Mobed-Miremadi ◽  
Korin E. Wheeler
Keyword(s):  
Machine Learning ◽  
Surface Chemistry ◽  
Physicochemical Properties ◽  
Silver Nanoparticle ◽  
Protein Corona ◽  
Engineered Nanomaterials ◽  
Predictive Analysis ◽  
Environmental Systems ◽  
Corona Formation ◽  
P Proteins

Proteins encountered in biological and environmental systems bind to engineered nanomaterials (ENMs) to form a protein corona (PC) that alters the surface chemistry, reactivity, and fate of the ENMs.

scholarly journals Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1028 ◽  
Author(s):  
Soyeon Jeon ◽  
Jessica Clavadetscher ◽  
Dong-Keun Lee ◽  
Sunay Chankeshwara ◽  
Mark Bradley ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Surface Charge ◽  
Cellular Uptake ◽  
A549 Cells ◽  
Protein Corona ◽  
Polystyrene Nanoparticles ◽  
Biological Impact ◽  
Corona Formation ◽  
Different Types

The evaluation of the role of physicochemical properties in the toxicity of nanoparticles is important for the understanding of toxicity mechanisms and for controlling the behavior of nanoparticles. The surface charge of nanoparticles is suggested as one of the key parameters which decide their biological impact. In this study, we synthesized fluorophore-conjugated polystyrene nanoparticles (F-PLNPs), with seven different types of surface functional groups that were all based on an identical core, to evaluate the role of surface charge in the cellular uptake of nanoparticles. Phagocytic differentiated THP-1 cells or non-phagocytic A549 cells were incubated with F-PLNP for 4 h, and their cellular uptake was quantified by fluorescence intensity and confocal microscopy. The amount of internalized F-PLNPs showed a good positive correlation with the zeta potential of F-PLNPs in both cell lines (Pearson’s r = 0.7021 and 0.7852 for zeta potential vs. cellular uptake in THP-1 cells and nonphagocytic A549 cells, respectively). This result implies that surface charge is the major parameter determining cellular uptake efficiency, although other factors such as aggregation/agglomeration, protein corona formation, and compositional elements can also influence the cellular uptake partly or indirectly.

scholarly journals Impact of silica nanoparticle surface chemistry on protein corona formation and consequential interactions with biological cells

2017 ◽  
Vol 75 ◽  
pp. 16-24 ◽  
Author(s):  
Andréa Kurtz-Chalot ◽  
Christian Villiers ◽  
Jérémie Pourchez ◽  
Delphine Boudard ◽  
Matteo Martini ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Silica Nanoparticle ◽  
Protein Corona ◽  
Biological Cells ◽  
Nanoparticle Surface ◽  
Corona Formation

scholarly journals The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids

Small ◽  
2017 ◽  
Vol 13 (16) ◽  
pp. 1603847 ◽  
Author(s):  
Alyssa B. Chinen ◽  
Chenxia M. Guan ◽  
Caroline H. Ko ◽  
Chad A. Mirkin
Keyword(s):  
Nucleic Acids ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Corona Formation ◽  
Spherical Nucleic Acids ◽  
The Impact

scholarly journals Surface properties modulate protein corona formation and determine cellular uptake and cytotoxicity of silver nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Marianna Barbalinardo ◽  
Jessika Bertacchini ◽  
Linda Bergamini ◽  
Maria Sara Magarò ◽  
Luca Ortolani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Properties ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Protein Corona ◽  
Diagnosis And Treatment ◽  
Corona Formation ◽  
Basic Understanding

Nanoparticles (NPs) have been studied for biomedical applications, ranging from prevention, diagnosis and treatment of diseases. However, the lack of the basic understanding of how NPs interact with the biological...

Protein Corona Formation on Colloidal Polymeric Nanoparticles and Polymeric Nanogels: Impact on Cellular Uptake, Toxicity, Immunogenicity, and Drug Release Properties

2017 ◽  
Vol 18 (6) ◽  
pp. 1762-1771 ◽  
Author(s):  
Katja Obst ◽  
Guy Yealland ◽  
Benjamin Balzus ◽  
Enrico Miceli ◽  
Mathias Dimde ◽  
...  
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Polymeric Nanoparticles ◽  
Protein Corona ◽  
Corona Formation

scholarly journals The Combination of Morphology and Surface Chemistry Defines the Biological Identity of Nanocarriers in Human Blood

2020 ◽  
Author(s):  
Nicholas B. Karabin ◽  
Michael P. Vincent ◽  
Sean D. Allen ◽  
Sharan Bobbala ◽  
Molly A. Frey ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Surface Chemistry ◽  
Human Blood ◽  
Rational Design ◽  
Immune Cell ◽  
Protein Corona ◽  
Blood Proteins ◽  
Drug Delivery Vehicles ◽  
Corona Formation ◽  
Delivery Vehicles

AbstractFollowing intravenous administration, an adsorbed corona of blood proteins immediately forms on the surfaces of nanocarriers to confer a distinct biological identity that dictates interactions with the immune system. While the nanocarrier surface chemistry has long been the focus of protein corona formation, the influence of the nanocarrier structure has remained unclear despite well-documented influences on biodistribution, clearance and inflammation. Here, we present design rules for the combined engineering of both nanocarrier structure and surface chemistry derived from a comprehensive proteomic analysis of protein corona formation in human blood. A library of nine soft PEGylated nanocarriers that differ in their combination of morphology (spheres, vesicles, and cylinders) and surface chemistry (methoxy, hydroxyl, and phosphate) were synthesized to represent properties of commonly employed drug delivery vehicles. Using label-free proteomics and high-throughput techniques, we examined the relationship between physicochemical properties and the resulting nanocarrier biological identity, including dynamic changes in protein corona composition, differential immunostimulation and uptake by relevant immune cell populations. In human blood, non-polar spherical micelles developed a similar biological identity to polar vesicles, whereas the identities of polar spheres and cylinders resembled that of non-polar vesicles. The formed protein coronas were compositionally dynamic and morphology-dependent, and these time-dependent fingerprints altered nanocarrier complement activation as well as their uptake by human monocytes, macrophages, and dendritic cells. This comprehensive analysis provides mechanistic insights into rational design choices that impact nanocarrier fate in human blood.One Sentence SummaryWe demonstrate that not only the surface chemistry, but the combined chemical and structural properties of soft drug delivery vehicles impact the composition of blood proteins that adsorb to their surfaces, and these differences specify their interactions with and modulation of human immune cells.

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