Application of three-dimensional liquid chromatography for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (THI) in caramel colours

2016 ◽  
Vol 8 (35) ◽  
pp. 6573-6579 ◽  
Author(s):  
Cédric Moretton ◽  
Henri Nigay
Keyword(s):  
Liquid Chromatography ◽  
Sample Preparation ◽  
Three Dimensional ◽  
New Method ◽  
Separation Power

This study described a new method to quantify THI in caramels colours without sample preparation thanks to the separation power of multidimensional liquid chromatography.

scholarly journals Enhancing biological analyses with three dimensional field asymmetric ion mobility, low field drift tube ion mobility and mass spectrometry (μFAIMS/IMS-MS) separations

The Analyst ◽  
2015 ◽  
Vol 140 (20) ◽  
pp. 6955-6963 ◽  
Author(s):  
Xing Zhang ◽  
Yehia M. Ibrahim ◽  
Tsung-Chi Chen ◽  
Jennifer E. Kyle ◽  
Randolph V. Norheim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Drift Tube ◽  
Three Dimensional ◽  
Separation Power ◽  
Low Field ◽  
Field Drift

Novel μFAIMS/IMS-MS three dimensional separations were optimized to enhance separation power and selectivity in biological analyses.

scholarly journals A New Method to Evaluate Trueness and Precision of Digital and Conventional Impression Techniques for Complete Dental Arch

2021 ◽  
Vol 11 (10) ◽  
pp. 4612
Author(s):  
KweonSoo Seo ◽  
Sunjai Kim
Keyword(s):  
Intraclass Correlation ◽  
Three Dimensional ◽  
New Method ◽  
Digital Impression ◽  
Dental Arch ◽  
Digital Models ◽  
Digital Impressions ◽  
Measuring Machine ◽  
Master Model ◽  
Conventional Impression

Purpose: The aim of this study was to present a new method to analyze the three-dimensional accuracy of complete-arch dental impressions and verify the reliability of the method. Additionally, the accuracies of conventional and intraoral digital impressions were compared using the new method. Methods: A master model was fabricated using 14 milled polyetheretherketone cylinders and a maxillary acrylic model. Each cylinder was positioned and named according to its corresponding tooth position. Twenty-five definitive stone casts were fabricated using conventional impressions of the master model. An intraoral scanner was used to scan the master model 25 times to fabricate 25 digital models. A coordinate measuring machine was used to physically probe each cylinder in the master model and definitive casts. An inspection software was used to probe cylinders of digital models. A three-dimensional part coordinate system was defined and used to compute the centroid coordinate of each cylinder. Intraclass correlation coefficient (ICC) was evaluated to examine the reliability of the new method. Independent two sample t-test was performed to compare the trueness and precision of conventional and intraoral digital impressions (α = 0.05). Results: ICC results showed that, the new method had almost perfect reliability for the measurements of the master model, conventional and digital impression. Conventional impression showed more accurate absolute trueness and precision than intraoral digital impression for most of the tooth positions (p < 0.05). Conclusions: The new method was reliable to analyze the three-dimensional deviation of complete-arch impressions. Conventional impression was still more accurate than digital intraoral impression for complete arches.

scholarly journals Towards spatial comprehensive three-dimensional liquid chromatography: a tutorial review

2020 ◽  
Author(s):  
Thomas Themelis ◽  
Ali Amini ◽  
Jelle De Vos ◽  
Sebastiaan Eeltink
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional

Determination of Free Solanesol Levels in Cigarette Filters by Liquid Chromatography–Mass Spectrometry

2021 ◽  
Author(s):  
Roberto Bravo Cardenas ◽  
Phuong Ngac ◽  
Clifford Watson ◽  
Liza Valentin-Blasini
Keyword(s):  
Liquid Chromatography ◽  
Sample Preparation ◽  
Tobacco Smoke ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Filter Material ◽  
Previous Method ◽  
Tobacco Smoke Exposure ◽  
Triple Quadrupole Mass Spectrometer ◽  
Cigarette Butts

Abstract Solanesol, a naturally occurring constituent of tobacco, has been utilized as a good marker for environmental tobacco smoke particulate and as a noninvasive predictor of mainstream cigarette smoke tar and nicotine intake under naturalistic smoking conditions. A fast and accurate method for measuring free solanesol to assess tobacco smoke exposure is highly desirable. We have developed and validated a new environmentally friendly, high-throughput method for measuring solanesol content in discarded cigarette filter butts. The solanesol deposited in the used filters can be correlated with mainstream smoke deliveries of nicotine and total particle matter to estimate constituent delivery to smokers. A portion of filter material is removed from cigarette butts after machine smoking, spiked with internal standard solution, extracted and quantitatively analyzed using reverse-phase liquid chromatography coupled to a triple-quadrupole mass spectrometer. The new method incorporates a 48-well plate format for automated sample preparation that reduces sample preparation time and solvent use and increases sample throughput 10-fold compared to our previous method. Accuracy and precision were evaluated by spiking known amounts of solanesol on both clean and smoked cigarette butts. Recoveries exceeded 93% at both low and high spiking levels. Linear solanesol calibration curves ranged from 1.9 to 367 µg/butt with a 0.05 µg/butt limit of detection.

A new method for volumetric measurement of the normal female cervix by three‐dimensional transvaginal ultrasound

2021 ◽  
Author(s):  
Matija Prka ◽  
Albert Despot ◽  
Alemka Brnčić Fischer ◽  
Herman Haller ◽  
Ana Tikvica Luetić ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Three Dimensional ◽  
New Method ◽  
Normal Female ◽  
Volumetric Measurement

scholarly journals Three-dimensional analysis of the root canal preparation with Reciproc Blue®, WaveOne Gold® and XP EndoShaper®: a new method in vivo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Nestor Rios-Osorio ◽  
Diana Usme ◽  
Cristian Jimenez ◽  
Adriana Pinzon ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Root Canal ◽  
Paired Comparison ◽  
Three Dimensional ◽  
New Method ◽  
Root Canal Preparation ◽  
Paired Data ◽  
Before And After ◽  
Student’S T

Abstract Background The purpose of this study was to evaluate the changes in canal volume after root canal preparation in vivo with 3 different single-file techniques (Reciproc-Blue®, WaveOne-Gold® and XP-EndoShaper®), with a new method using CBCT and 3D reconstruction. Methods In this prospective study, thirty human lower premolars from healthy patients were used, in which extraction was indicated for orthodontic reasons. All the teeth used were caries- and restoration-free with complete root development, without signs of periodontal disease or traumatic occlusion, and with only one straight canal (up to 25º curvature). Teeth were randomly divided into three different groups: Reciproc-Blue, WaveOne-Gold and XP-EndoShaper. CBCT scans before root canal preparation were used to create a 3D reconstruction with RHINOCEROS 5.0 software to assess the initial canal volume, and then compared with 3D reconstructions after canal preparation to measure the increase in canal volume. Student’s t test for paired data were used to determine statistically significant differences between the before and after canal volumes. Anova test was used to determine statistically significant differences in the percentage of canal volume increase between the groups and Tukey's post-hoc test were used to paired comparison. Results Reciproc-Blue showed the higher increase in canal volume, followed by WaveOne-Gold and XP-EndoShaper (p = 0.003). XP-EndoShaper did not show a statistically significant increase in canal volume after root canal preparation (p = 0.06). Conclusion With this model, Reciproc-Blue showed higher increase in root canal volume, followed by WaveOne-Gold, while XP-EndoShaper did not significantly increase root canal volume during preparation.

Integration of Liquid Chromatography with Immunoassay: An Approach Combining the Strengths of Both Methods

1994 ◽  
Vol 77 (5) ◽  
pp. 1275-1287 ◽  
Author(s):  
Petra M Krämer ◽  
Qing X Li ◽  
Bruce D Hammock
Keyword(s):  
Liquid Chromatography ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Uv Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multiresidue Analysis ◽  
Selective Method ◽  
Separation Power ◽  
Immunochemical Detection ◽  
Very High

Abstract The integration of liquid chromatography (LC) with immunochemical detection combines the superior separation power of LC and the sensitivity and specificity of immunoassays. This approach is shown with 3 LC systems (Perkin-Elmer, C18 RP, 4.6 mm; Varian, C18 RP, 1 mm microbore; Michrom, C18 RP, 1 mm microbore) Integrated with an enzyme-linked immunosorbent assay (ELISA) selective for five 4-nitrophenols. The nitrophenols were separated with the 3 LC systems with isocratic runs of 15 to 20 min. Microbore LC separation showed a 10-20 times reduction in solvent amount compared to conventional separation. LC–immunoassay was about 8- to 10-fold more sensitive compared with LC with UV detection. Integrated LC–immunoassay proved to be a very selective method when 2-methylphenol was injected with an equimolar mixture of 2-amino-4-nitrophenol and 3-methyl-4-nrtrophenol; 2-methy I phenol does not crossreact with the serum used. Only 2 peaks could be seen in the detection, even when 2-methylphenol was present in very high amounts (3000 pmol). Further, the EUSA-LC detection proved to be selective and sensitive for complex matrixes. 2-Amlno-4-nitrophenol was clearly identified in spiked extracts of soil and plant, even when a very small amount (2.4 ng) was injected. Although LC–immunoassay is more labor intensive than LC with UV detection, it offers great advantages in multiresidue analysis and is generally applicable for peak confirmation.

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