scholarly journals Towards spatial comprehensive three-dimensional liquid chromatography: a tutorial review

2020 ◽  
Author(s):  
Thomas Themelis ◽  
Ali Amini ◽  
Jelle De Vos ◽  
Sebastiaan Eeltink
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional

Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph

2017 ◽  
Vol 2 (30) ◽  
pp. 9797-9802
Author(s):  
Eva Sarkadi-Priboczki ◽  
Ivan Valastyan ◽  
Karoly Brezovcsik ◽  
David Nagy ◽  
Gabor Opposits ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Three Dimensional ◽  
Positron Emission Tomograph ◽  
Three Dimensional Imaging ◽  
Positron Emission

Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides

2012 ◽  
Vol 393 (8) ◽  
pp. 757-765 ◽  
Author(s):  
Weston B. Struwe ◽  
Pauline M. Rudd
Keyword(s):  
Liquid Chromatography ◽  
Hplc Analysis ◽  
Three Dimensional ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Peak Capacity ◽  
Fluorescent Labels ◽  
Sample Analysis ◽  
Hydrophilic Interaction ◽  
Optimal Excitation ◽  
Complex Sample

Abstract In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differed considerably from the wavelengths applied in previous reports. HILIC-FLD chromatograms using experimentally determined wavelengths were similar to 2-aminobenzamide controls, but the peak capacity and resolution differed significantly when published 3-AQ λex/em values were applied. Furthermore, we found that 5-AQ and 8-AQ labeled maltohexaose did not display any fluorescent pro\xadperties when used as a carbohydrate tag for HPLC analysis. Finally, we applied experimentally determined wavelengths to 3-AQ labeled N-glycans released from human IgG to illustrate changes in retention time as well as to demonstrate that AQ labeling is applicable to complex sample analysis via exoglycosidase sequencing.

Application of three-dimensional liquid chromatography for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (THI) in caramel colours

2016 ◽  
Vol 8 (35) ◽  
pp. 6573-6579 ◽  
Author(s):  
Cédric Moretton ◽  
Henri Nigay
Keyword(s):  
Liquid Chromatography ◽  
Sample Preparation ◽  
Three Dimensional ◽  
New Method ◽  
Separation Power

This study described a new method to quantify THI in caramels colours without sample preparation thanks to the separation power of multidimensional liquid chromatography.

Liquid chromatography mass spectrometry based characterization of epitope configurations

2020 ◽  
Vol 12 (45) ◽  
pp. 5476-5484
Author(s):  
Maren Christin Stillesby Levernæs ◽  
Arelí Urtubia Moe ◽  
Sigurd Leinæs Bøe ◽  
Elisabeth Paus ◽  
Léon Reubsaet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Liquid Chromatography Mass Spectrometry ◽  
Three Dimensional Structure ◽  
Chromatography Mass Spectrometry ◽  
Pre Treatment

Here we evaluate a quick and easy tool for determination of epitope configuration using immunocapture and liquid chromatography mass spectrometry (LC-MS) subsequent to pre-treatment of the target protein to disrupt its three-dimensional structure.

Fractionation of Complex Protein Mixture by Virtual Three-Dimensional Liquid Chromatography Based on Combined pH and Salt Steps

2008 ◽  
Vol 7 (10) ◽  
pp. 4525-4537 ◽  
Author(s):  
Zhi-Bin Ning ◽  
Qing-Run Li ◽  
Jie Dai ◽  
Rong-Xia Li ◽  
Chia-Hui Shieh ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein
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