scholarly journals Push–pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand–receptor interactions

2016 ◽  
Vol 4 (14) ◽  
pp. 3002-3009 ◽  
Author(s):  
Iuliia A. Karpenko ◽  
Yosuke Niko ◽  
Viktor P. Yakubovskyi ◽  
Andriy O. Gerasov ◽  
Dominique Bonnet ◽  
...  
Keyword(s):  
Molecular Rotors ◽  
Live Cells ◽  
Molecular Rotor ◽  
Solvatochromic Dyes ◽  
Fluorogenic Probe ◽  
Receptor Interactions ◽  
Fluorogenic Probes ◽  
Detection Of Biomolecules

Fluorescent solvatochromic dyes and molecular rotors have attracted considerable attention as fluorogenic probes because of background-free detection of biomolecules in live cells in no-wash conditions.

The effect of solvent polarity and macromolecular crowding on the viscosity sensitivity of a molecular rotor BODIPY-C10

2020 ◽  
Vol 22 (16) ◽  
pp. 8296-8303
Author(s):  
Artūras Polita ◽  
Stepas Toliautas ◽  
Rokas Žvirblis ◽  
Aurimas Vyšniauskas
Keyword(s):  
Biological Systems ◽  
Macromolecular Crowding ◽  
Solvent Polarity ◽  
Fluorescent Sensors ◽  
Molecular Rotors ◽  
Live Cells ◽  
Molecular Rotor ◽  
Effect Of Solvent ◽  
Key Parameter

Viscosity is the key parameter of many biological systems such as live cells. It can be conveniently measured with ‘molecular rotors’ – fluorescent sensors of microviscosity. Here, we investigate one of the most applied molecular rotors BODIPY-C10.

A ratiometric fluorogenic probe for the real-time detection of SO32−in aqueous medium: application in a cellulose paper based device and potential to sense SO32−in mitochondria

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 250-257 ◽  
Author(s):  
Soham Samanta ◽  
Senjuti Halder ◽  
Poulomi Dey ◽  
Utsab Manna ◽  
Aiyagari Ramesh ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Real Time ◽  
Water Soluble ◽  
Live Cells ◽  
The Real ◽  
Cellulose Paper ◽  
Fluorogenic Probe ◽  
Ratiometric Detection ◽  
Real Time Detection

A new water soluble and fluorogenic probe (L) that can demonstrate the specific ratiometric detection of a SO2derivative (SO32−) in 100% aqueous medium and live cells has been designed and synthesized.

Molecular Rotors as Reporters for Viscosity of Solutions of Collagen Like Peptides

2021 ◽  
Author(s):  
Christopher McTiernan ◽  
Matias Zuñiga-Bustos ◽  
Roberto Rosales-Rojas ◽  
Pablo Barrias ◽  
May Griffith ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorometric Assay ◽  
Molecular Rotors ◽  
Molecular Rotor

We have studied the suitability of using a molecular rotor-based steady-state fluorometric assay for evaluating changes in both the conformation and the viscosity of collagen-like peptide solutions. Our results indicate...

scholarly journals A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells

2008 ◽  
Vol 18 (22) ◽  
pp. 5864-5866 ◽  
Author(s):  
Melissa M. Yatzeck ◽  
Luke D. Lavis ◽  
Tzu-Yuan Chao ◽  
Sunil S. Chandran ◽  
Ronald T. Raines
Keyword(s):  
Cytochrome P450 ◽  
Live Cells ◽  
Cytochrome P450 Activity ◽  
Fluorogenic Probe ◽  
Highly Sensitive ◽  
P450 Activity

scholarly journals A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region

2018 ◽  
Vol 24 (24) ◽  
pp. 6344-6348 ◽  
Author(s):  
Cecilia Samaniego Lopez ◽  
Jimena Hebe Martínez ◽  
María Laura Uhrig ◽  
Federico Coluccio Leskow ◽  
Carla Cecilia Spagnuolo
Keyword(s):  
Near Infrared ◽  
Infrared Region ◽  
Live Cells ◽  
Fluorogenic Probe ◽  
Highly Sensitive ◽  
Near Infrared Region

Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3433-3441 ◽  
Author(s):  
Yanfei Zhao ◽  
Yun Ni ◽  
Liulin Wang ◽  
Chenchen Xu ◽  
Chenqi Xin ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Live Cell ◽  
Live Cells ◽  
Two Photon ◽  
Ligand Displacement ◽  
Cell Tissue ◽  
Fluorogenic Probe ◽  
Displacement Based

We report the Fe(iii)-based complex TPFeS which acts as a novel ligand-displacement-based TP fluorogenic probe for the rapid detection of mercapto biomolecules both in vitro and in live cell/tissue/in vivo imaging.

Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme activities in live cells

2019 ◽  
Vol 55 (99) ◽  
pp. 15000-15003 ◽  
Author(s):  
Jialing Jiang ◽  
Qingwei Tan ◽  
Shuxuan Zhao ◽  
Heng Song ◽  
Liqiang Hu ◽  
...  
Keyword(s):  
Late Stage ◽  
Enzyme Activities ◽  
Quinone Methide ◽  
Live Cells ◽  
Fluorogenic Probe

A novel p-quinone methide-based self-immobilizing and fluorogenic probe is reported for the visualization of β-galactosidase activities in live cells.

scholarly journals Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification

1997 ◽  
Vol 43 (5) ◽  
pp. 752-758 ◽  
Author(s):  
Stefania Gelmini ◽  
Claudio Orlando ◽  
Roberta Sestini ◽  
Giovanna Vona ◽  
Pamela Pinzani ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Fluorescence Emission ◽  
Globin Genes ◽  
Time Saving ◽  
Taq Polymerase ◽  
Oncogene Amplification ◽  
Fluorogenic Probe ◽  
Pcr Product ◽  
Fluorogenic Probes ◽  
Homogeneous Assay

Abstract We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqManTM system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and β-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase of the PCR cycle, the 5′→3′ exonuclease activity of Taq polymerase cleaves the hybridized fluorogenic probe, resulting in an increase of fluorescence emission of the reporter dye that is quantitative for the amount of PCR product and, under appropriate conditions, for the amount of template. Assay performance showed adequate precision and a lower detection limit and good correlation with the results obtained in the same samples by a competitive PCR assay (n = 25, r = 0.94, P <0.01). This homogeneous assay is time-saving, avoids usually cumbersome postamplification procedures (that can be additional sources of inaccuracy and contamination), and seems suitable for determination of c-erbB-2 oncogene amplification in tumor specimens.

A fluorogenic probe for imaging protein S-nitrosylation in live cells

2017 ◽  
Vol 94 ◽  
pp. 162-168 ◽  
Author(s):  
Shiyi Shao ◽  
Bo Chen ◽  
Juan Cheng ◽  
Chengkun Wang ◽  
Yanli Zhang ◽  
...  
Keyword(s):  
Protein S ◽  
Live Cells ◽  
Fluorogenic Probe

Predicting lanthanide boride inverse sandwich tubular molecular rotors with the smallest core–shell structure

Nanoscale ◽  
2019 ◽  
Vol 11 (44) ◽  
pp. 21311-21316 ◽  
Author(s):  
Xiao-Qin Lu ◽  
Qiang Chen ◽  
Xin-Xin Tian ◽  
Yue-Wen Mu ◽  
Hai-Gang Lu ◽  
...  
Keyword(s):  
First Principles ◽  
Shell Structure ◽  
Molecular Rotors ◽  
Core Shell ◽  
Molecular Rotor ◽  
Sandwich Complexes ◽  
Core Shell Structure

Extensive first-principles theory calculations predict the possibility of bi-decker inverse sandwich complexes La2[B18], La2[B18]2−, and La2[B2@B18], with the B2-centered La2[B2@B18] as a tubular molecular rotor possessing the smallest core–shell structure.

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