Time evolution and dynamic cellular uptake of PEGYlated gold nanorods

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 8089-8092 ◽  
Author(s):  
Hongyuan Song ◽  
Qingqiang Xu ◽  
Hongwei Di ◽  
Ting Guo ◽  
Zhongtian Qi ◽  
...  
Keyword(s):  
Time Evolution ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Protein Corona

The protein corona formed and evolved of PEGYlated GNRs which alter GNRs characteristics significantly leading to dynamic cellular uptake pathways.

scholarly journals Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

Nanoscale ◽  
2016 ◽  
Vol 8 (13) ◽  
pp. 6948-6957 ◽  
Author(s):  
Marilena Hadjidemetriou ◽  
Zahraa Al-Ahmady ◽  
Kostas Kostarelos
Keyword(s):  
Time Evolution ◽  
Liposomal Doxorubicin ◽  
Pegylated Liposomal Doxorubicin ◽  
Protein Corona

Time evolution of β-lactoglobulin corona formation on gold nanoparticles and impact on allergy

2020 ◽  
Author(s):  
Xiaoning Zhang ◽  
Meifeng Li ◽  
Yuanping Lv ◽  
Xiaoling Sun ◽  
Yao Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Secondary Structure ◽  
Time Evolution ◽  
Electrostatic Force ◽  
Protein Corona ◽  
Raw Milk ◽  
Covalent Bonds ◽  
Corona Formation ◽  
Β Lactoglobulin ◽  
Β Sheet

Abstract Gold nanoparticles (AuNPs) are modified immediately by the adsorption of β-lactoglobulin (βlg) when designed as colorimetric probe in raw milk, leading to the formation of a protein corona. This adsorption results mainly from a fast electrostatic force and a slow formation of Au-S covalent bonds, which is a precondition for the use of AuNPs in biodetection. The proteins corona influences the structure and bioactivity of adsorbed protein, such as the allergy. In this study, the mechanism of βlg adsorbed on AuNPs was investigated in terms of stoichiometry, binding affinity (Ka), time evolution of Au-S bond, and general secondary structure changes to address the desensitization of AuNPs. The results show that about 3,600 βlg are adsorbed on a single AuNPs, and the Ka is 2.9 ± 0.7 × 10 6 M -1 . The formation of Au-S bonds takes about 9 h, which is the time needed for complete changes in secondary structure and the IgE combining capacity. The structure of allergenic epitopes assigned to β-sheet was destroyed by the formation of Au-S bond, then induced to the decrease allergy. Furthermore, Fourier transform infrared spectroscopy confirmed a decrease in β-sheet contents after conjugated with AuNPs.

scholarly journals Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2250
Author(s):  
Shanmugavel Chinnathambi ◽  
Nobutaka Hanagata ◽  
Tomohiko Yamazaki ◽  
Naoto Shirahata
Keyword(s):  
Quantum Dots ◽  
Blood Plasma ◽  
Cellular Uptake ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Infrared Light ◽  
Spectral Window ◽  
Blood Plasma Proteins

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein’s secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellular uptake (NIH3T3 Fibroblasts) and minimal cytotoxicity using different cell lines (HeLa, A549, and NIH3T3). This study offers a basis to design QDs without altering the biomacromolecule’s original conformation with enabled cellular uptake with minimal cytotoxicity.

Protein corona formation and its influence on biomimetic magnetite nanoparticles

2020 ◽  
Vol 8 (22) ◽  
pp. 4870-4882 ◽  
Author(s):  
Ana Peigneux ◽  
Emanuel A. Glitscher ◽  
Rawan Charbaji ◽  
Christoph Weise ◽  
Stefanie Wedepohl ◽  
...  
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Magnetite Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

Colloidal stability and cellular uptake of MamC-biomimetic magnetite nanoparticles (BMNPs) incubated with human plasma (PC-BMNPs).

Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake

Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23259-23267 ◽  
Author(s):  
Alberto Piloni ◽  
Chin Ken Wong ◽  
Fan Chen ◽  
Megan Lord ◽  
Andreas Walther ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Biological Behavior ◽  
Protein Absorption ◽  
Corona Formation

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.

Cellular Uptake and Cytotoxicity of Varying Aspect Ratios of Gold Nanorods in HeLa Cells

2020 ◽  
Vol 3 (3) ◽  
pp. 1374-1384 ◽  
Author(s):  
Deshani Fernando ◽  
Shoukath Sulthana ◽  
Yolanda Vasquez
Keyword(s):  
Cellular Uptake ◽  
Gold Nanorods ◽  
Hela Cells ◽  
Aspect Ratios

Preparation of the protein corona: How washing shapes the proteome and influences cellular uptake of nanocarriers

2020 ◽  
Vol 114 ◽  
pp. 333-342
Author(s):  
Maximilian Brückner ◽  
Johanna Simon ◽  
Shuai Jiang ◽  
Katharina Landfester ◽  
Volker Mailänder
Keyword(s):  
Cellular Uptake ◽  
Protein Corona

Time Evolution of the Nanoparticle Protein Corona

ACS Nano ◽  
2010 ◽  
Vol 4 (7) ◽  
pp. 3623-3632 ◽  
Author(s):  
Eudald Casals ◽  
Tobias Pfaller ◽  
Albert Duschl ◽  
Gertie Janneke Oostingh ◽  
Victor Puntes
Keyword(s):  
Time Evolution ◽  
Protein Corona

Surface modification of gold nanorods using layer-by-layer technique for cellular uptake

2007 ◽  
Vol 10 (1) ◽  
pp. 221-228 ◽  
Author(s):  
Hironobu Takahashi ◽  
Takuro Niidome ◽  
Takahito Kawano ◽  
Sunao Yamada ◽  
Yasuro Niidome
Keyword(s):  
Surface Modification ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Layer By Layer ◽  
Layer Technique ◽  
Layer By Layer Technique
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