Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using β-galactosidase from Aspergillus sp.

RSC Advances ◽  
2015 ◽  
Vol 5 (96) ◽  
pp. 78874-78879 ◽  
Author(s):  
Hui-da Wan ◽  
Dan Li
Keyword(s):  
Ginsenoside Rb1 ◽  
Ginsenoside Rh2 ◽  
Ginsenoside Rg3 ◽  
Ginsenoside Rd ◽  
Highly Efficient ◽  
Rare Ginsenoside ◽  
Aspergillus Sp

β-Galactosidase from Aspergillus sp. can transform major ginsenoside Rb1 to rare ginsenoside F2 via ginsenoside Rd. Ginsenoside Rg3 can be selectively hydrolyzed with this β-galactosidase and only ginsenoside Rh2 was obtained as well.

scholarly journals Development of a Validated UPLC-MS/MS Method for Analyzing Major Ginseng Saponins from Various Ginseng Species

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4065 ◽  
Author(s):  
Ling Yang ◽  
Chi-Lin Li ◽  
Yung-Yi Cheng ◽  
Tung-Hu Tsai
Keyword(s):  
Partition Coefficient ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Reversed Phase ◽  
Hydroxyl Groups ◽  
Ginsenoside Rb1 ◽  
Ginsenoside Re ◽  
Ginsenoside Rd

Ginsenosides, which contain one triterpene and one or more sugar moieties, are the major bioactive compounds of ginseng. The aim of this study was to develop and optimize a specific and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the analysis of twelve different resources of ginseng. The six marker compounds of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, and ginsenoside Rg1, as well as an internal standard, were separated by a reversed-phase C-18 column with a gradient elution of water and methanol-acetonitrile. The multiple-reaction monitoring (MRM) mode was used to quantify and identify twelve market products. The results demonstrated that not only is the logarithm of its partition coefficient (cLog P; octanol-water partition coefficient) one of the factors, but also the number of sugars, position of sugars, and position of the hydroxyl groups are involved in the complicated separation factors for the analytes in the analytical system. If the amount of ginsenoside Rb1 was higher than 40 mg/g, then the species might be Panax quinquefolius, based on the results of the marker ginsenoside contents of various varieties. In summary, this study provides a rapid and precise analytical method for identifying the various ginsenosides from different species, geographic environments, and cultivation cultures.

Conversion of major ginsenoside Rb1 to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514

2008 ◽  
Vol 69 (1) ◽  
pp. 218-224 ◽  
Author(s):  
Le-Qin Cheng ◽  
Ju Ryun Na ◽  
Myun Ho Bang ◽  
Myung Kyum Kim ◽  
Deok-Chun Yang
Keyword(s):  
Ginsenoside Rb1 ◽  
Ginsenoside Rg3

scholarly journals Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1

2015 ◽  
Vol 39 (4) ◽  
pp. 304-313 ◽  
Author(s):  
Hoang Tung Vo ◽  
Jae Youl Cho ◽  
Yong-Eui Choi ◽  
Yong-Soon Choi ◽  
Yeon-Ho Jeong
Keyword(s):  
Heat Treatment ◽  
Kinetic Study ◽  
Ginsenoside Rb1 ◽  
Ginsenoside Rg3

Effects of external calcium on the biotransformation of ginsenoside Rb1 to ginsenoside Rd by Paecilomyces bainier 229-7

2011 ◽  
Vol 28 (3) ◽  
pp. 857-863 ◽  
Author(s):  
Li Ye ◽  
Chunyan Zhang ◽  
Jiyang Li ◽  
Xunlong Shi ◽  
Meiqing Feng
Keyword(s):  
Ginsenoside Rb1 ◽  
Ginsenoside Rd ◽  
External Calcium

scholarly journals Biocatalysis for Rare Ginsenoside Rh2 Production in High Level with Co-Immobilized UDP-Glycosyltransferase Bs-YjiC Mutant and Sucrose Synthase AtSuSy

Catalysts ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 132
Author(s):  
Jianlin Chu ◽  
Jiheng Yue ◽  
Song Qin ◽  
Yuqiang Li ◽  
Bin Wu ◽  
...  
Keyword(s):  
Sucrose Synthase ◽  
Specific Binding ◽  
High Titer ◽  
Substrate Inhibition ◽  
Ginsenoside Rh2 ◽  
Recombinant Enzymes ◽  
One Pot ◽  
Final Yield ◽  
Rare Ginsenoside ◽  
High Level

Rare ginsenoside Rh2 exhibits diverse pharmacological effects. UDP-glycosyltransferase (UGT) catalyzed glycosylation of protopanaxadiol (PPD) has been of growing interest in recent years. UDP-glycosyltransferase Bs-YjiC coupling sucrose synthase in one-pot reaction was successfully applied to ginsenoside biosynthesis with UDP-glucose regeneration from sucrose and UDP, which formed a green and sustainable approach. In this study, the his-tagged UDP-glycosyltransferase Bs-YjiC mutant M315F and sucrose synthase AtSuSy were co-immobilized on heterofunctional supports. The affinity adsorption significantly improved the capacity of specific binding of the two recombinant enzymes, and the dual enzyme covalently cross-linked by the acetaldehyde groups significantly promoted the binding stability of the immobilized bienzyme, allowing higher substrate concentration by easing substrate inhibition for the coupled reaction. The dual enzyme amount used for ginsenoside Rh2 biosynthesis is Bs-YjiC-M315F: AtSuSy = 18 mU/mL: 25.2 mU/mL, a yield of 79.2% was achieved. The coimmobilized M315F/AtSuSy had good operational stability of repetitive usage for 10 cycles, and the yield of ginsenoside Rh2 was kept between 77.6% and 81.3%. The high titer of the ginsenoside Rh2 cumulatively reached up to 16.6 mM (10.3 g/L) using fed-batch technology, and the final yield was 83.2%. This study has established a green and sustainable approach for the production of ginsenoside Rh2 in a high level of titer, which provides promising candidates for natural drug research and development.

scholarly journals Conversion of Protopanaxadiol Type Saponins to Ginsenoside Rg3 by Lemon

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Cheng-Peng Sun ◽  
Wei-Ping Gao ◽  
Bao-Zhong Zhao ◽  
Le-Qin Cheng
Keyword(s):  
Nmr Spectroscopy ◽  
Lemon Juice ◽  
Citrus Limon ◽  
Ginsenoside Rb1 ◽  
Ginsenoside Rg3 ◽  
H Nmr ◽  
Strong Ability ◽  
C Nmr

The ability of fresh lemon ( Citrus limon) to convert protopanaxadiol-type saponins into ginsenoside Rg3 was investigated, and the structures of 20( S)-ginsenoside Rg3 (1) and 20( R)-ginsenoside Rg3 (2) were identified by 1H NMR and 13C NMR spectroscopy. The experiment showed that lemon possesses the strong ability to hydrolyze ginsenosides. When protopanaxadiol-type saponins (16 mg/mL) were hydrolyzed by fresh lemon juice at 80°C for 3 hrs, the conversion ratios of ginsenoside Rb1, Rb2, Rc and Rd were 92.9%, 90.0%, 96.9% and 55.5%, respectively, and the yields of 20( S)-ginsenoside Rg3 and 20( R)-ginsenoside Rg3 were, respectively, 31.2% and 28.3%.

Sign in / Sign up

Export Citation Format

Share Document