A ratiometric lysosomal pH probe based on the coumarin–rhodamine FRET system

RSC Advances ◽  
2015 ◽  
Vol 5 (61) ◽  
pp. 49115-49121 ◽  
Author(s):  
Xiao-Fan Zhang ◽  
Tao Zhang ◽  
Shi-Li Shen ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Lysosomal Ph ◽  
Ph Probe ◽  
System P

The ratiometric pH probe RC1 was constructed by integrating a coumarin moiety as a FRET donor into a rhodamine moiety as a FRET acceptor. The probe with a pKa of 4.98 could detect pH in the range of 4.20–6.00 and selectively stain lysosomes.

A ratiometric lysosomal pH probe based on the imidazo[1,5-a]pyridine–rhodamine FRET and ICT system

RSC Advances ◽  
2016 ◽  
Vol 6 (47) ◽  
pp. 41317-41322 ◽  
Author(s):  
Guang-Jie Song ◽  
Su-Yun Bai ◽  
Xi Dai ◽  
Xiao-Qun Cao ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Lysosomal Ph ◽  
Ph Probe ◽  
System P ◽  
Ict System ◽  
Low Cytotoxicity ◽  
Ratiometric Fluorescent Probe

A new pH-activatable ratiometric fluorescent probe (RhMP) has been developed based on FRET. This probe displayed good selectivity, and excellent reversibility. In addition, RhMP has low cytotoxicity and has been successfully applied in HeLa cells.

A ratiometric lysosomal pH probe based on the naphthalimide–rhodamine system

2015 ◽  
Vol 3 (16) ◽  
pp. 3260-3266 ◽  
Author(s):  
Xiao-Fan Zhang ◽  
Tao Zhang ◽  
Shi-Li Shen ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe ◽  
System P

The ratiometric pH probe RNL was constructed by integrating the naphthalimide moiety as an FRET donor into the rhodamine moiety as an FRET acceptor. The probe with a pKa of 4.82 could detect pH in the range of 4.50–5.50, selectively stain lysosome and detect lysosomal pH changes.

A near-infrared lysosomal pH probe based on rhodamine derivative

2018 ◽  
Vol 256 ◽  
pp. 261-267 ◽  
Author(s):  
Shi-Li Shen ◽  
Xiao-Fan Zhang ◽  
Yan-Qing Ge ◽  
Yan Zhu ◽  
Xue-Qin Lang ◽  
...  
Keyword(s):  
Near Infrared ◽  
Lysosomal Ph ◽  
Ph Probe ◽  
Rhodamine Derivative

Visual monitoring of the lysosomal pH changes during autophagy with a red-emission fluorescent probe

2020 ◽  
Vol 8 (7) ◽  
pp. 1466-1471 ◽  
Author(s):  
Xiaodong Wang ◽  
Li Fan ◽  
Yubin Wang ◽  
Caihong Zhang ◽  
Wenting Liang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Visual Monitoring ◽  
Red Emission ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe

We report a red-emission fluorescent pH probe (RML) for visual monitoring of the lysosomal pH changes during autophagy in living cells.

Imaging of lysosomal pH changes with a novel quinoline/benzothiazole probe

2018 ◽  
Vol 42 (16) ◽  
pp. 13479-13485 ◽  
Author(s):  
Li Fan ◽  
Ming Nan ◽  
Jinyin Ge ◽  
Xiaodong Wang ◽  
Bo Lin ◽  
...  
Keyword(s):  
Live Cells ◽  
E Coli ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe ◽  
Ph Fluctuations

A fluorescent pH probe BTVQ for imaging lysosomal pH fluctuations in live cells and extreme acidity in E. coli cells.

A rhodamine B-based lysosomal pH probe

2015 ◽  
Vol 3 (5) ◽  
pp. 919-925 ◽  
Author(s):  
Shi-Li Shen ◽  
Xin-Peng Chen ◽  
Xiao-Fan Zhang ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Response Time ◽  
Rhodamine B ◽  
High Selectivity ◽  
High Sensitivity ◽  
Living Cells ◽  
Acidic Ph ◽  
Ph Change ◽  
Short Response Time ◽  
Lysosomal Ph ◽  
Ph Probe

A novel rhodamine B-based lysosomal pH probe RML was developed. RML responded to acidic pH with short response time, high selectivity and high sensitivity and could detect lysosomal pH change in living cells.

Comparison of TEM and STM observations of small silver clusters on different carbon supports

1990 ◽  
Vol 48 (4) ◽  
pp. 264-265
Author(s):  
Bernd Tesche ◽  
Tobias Schilling
Keyword(s):  
Vibration Isolation ◽  
Pyrolytic Graphite ◽  
Carbon Film ◽  
Silver Clusters ◽  
Carbon Supports ◽  
Stacking Order ◽  
System P ◽  
High Resolution Tem ◽  
High Degree ◽  
Degree Of Order

The objective of our work is to determine:a) whether both of the imaging methods (TEM, STM) yield comparable data andb) which method is better suited for a reliable structure analysis of microclusters smaller than 1.5 nm, where a deviation of the bulk structure is expected.The silver was evaporated in a bell-jar system (p 10−5 pa) and deposited onto a 6 nm thick amorphous carbon film and a freshly cleaved highly oriented pyrolytic graphite (HOPG).The average deposited Ag thickness is 0.1 nm, controlled by a quartz crystal microbalance at a deposition rate of 0.02 nm/sec. The high resolution TEM investigations (100 kV) were executed by a hollow-cone illumination (HCI). For the STM investigations a commercial STM was used. With special vibration isolation we achieved a resolution of 0.06 nm (inserted diffraction image in Fig. 1c). The carbon film shows the remarkable reduction in noise by using HCI (Fig. 1a). The HOPG substrate (Fig. 1b), cleaved in sheets thinner than 30 nm for the TEM investigations, shows the typical arrangement of a nearly perfect stacking order and varying degrees of rotational disorder (i.e. artificial single crystals). The STM image (Fig. 1c) demonstrates the high degree of order in HOPG with atomic resolution.

Conclusion: What is Speculative Empiricism?

2018 ◽  
Author(s):  
Didier Debaise
Keyword(s):  
Social Groups ◽  
Speculative Philosophy ◽  
System P ◽  
History Of ◽  
Selection Of

Process and Reality ends with a warning: ‘[t]he chief danger to philosophy is narrowness in the selection of evidence’ (PR, 337). Although this danger of narrowness might emerge from the ‘idiosyncrasies and timidities of particular authors, of particular social groups, of particular schools of thought, of particular epochs in the history of civilization’ (PR, 337), we should not be mistaken: it occurs within philosophy, in its activity, its method. And the fact that this issue arises at the end of Process and Reality reveals the ambition that has accompanied its composition: Whitehead has resisted this danger through the form and ambition of his speculative construction. The temptation of a narrowness in selection attempts to expel speculative philosophy at the same time as it haunts each part of its system.

Pedestrian Oriented Vehicular Collision Avoidance Support System: P-VCASS

2010 ◽  
Vol E93-A (4) ◽  
pp. 679-688 ◽  
Author(s):  
Tomotaka WADA ◽  
Yuki NAKANISHI ◽  
Ryohta YAMAGUCHI ◽  
Kazushi FUJIMOTO ◽  
Hiromi OKADA
Keyword(s):  
Collision Avoidance ◽  
Support System ◽  
System P
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