Imaging of lysosomal pH changes with a novel quinoline/benzothiazole probe

2018 ◽  
Vol 42 (16) ◽  
pp. 13479-13485 ◽  
Author(s):  
Li Fan ◽  
Ming Nan ◽  
Jinyin Ge ◽  
Xiaodong Wang ◽  
Bo Lin ◽  
...  
Keyword(s):  
Live Cells ◽  
E Coli ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe ◽  
Ph Fluctuations

A fluorescent pH probe BTVQ for imaging lysosomal pH fluctuations in live cells and extreme acidity in E. coli cells.

A naphthalene-based fluorescent probe with a large Stokes shift for mitochondrial pH imaging

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 5054-5060 ◽  
Author(s):  
Bo Lin ◽  
Li Fan ◽  
Jinyin Ge ◽  
Wenjia Zhang ◽  
Caihong Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
Live Cells ◽  
Ph Changes ◽  
Large Stokes Shift ◽  
Ph Probe ◽  
Ph Imaging

A naphthalene-based fluorescent pH probe with a pKa of 8.8 for imaging mitochondrial pH changes in live cells.

A ratiometric lysosomal pH probe based on the naphthalimide–rhodamine system

2015 ◽  
Vol 3 (16) ◽  
pp. 3260-3266 ◽  
Author(s):  
Xiao-Fan Zhang ◽  
Tao Zhang ◽  
Shi-Li Shen ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe ◽  
System P

The ratiometric pH probe RNL was constructed by integrating the naphthalimide moiety as an FRET donor into the rhodamine moiety as an FRET acceptor. The probe with a pKa of 4.82 could detect pH in the range of 4.50–5.50, selectively stain lysosome and detect lysosomal pH changes.

A novel far-visible and near-infrared pH probe for monitoring near-neutral physiological pH changes: imaging in live cells

2013 ◽  
Vol 1 (34) ◽  
pp. 4281 ◽  
Author(s):  
Li Fan ◽  
Qiaoling Liu ◽  
Dongtao Lu ◽  
Heping Shi ◽  
Yunfeng Yang ◽  
...  
Keyword(s):  
Near Infrared ◽  
Live Cells ◽  
Ph Changes ◽  
Ph Probe

Visual monitoring of the lysosomal pH changes during autophagy with a red-emission fluorescent probe

2020 ◽  
Vol 8 (7) ◽  
pp. 1466-1471 ◽  
Author(s):  
Xiaodong Wang ◽  
Li Fan ◽  
Yubin Wang ◽  
Caihong Zhang ◽  
Wenting Liang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Visual Monitoring ◽  
Red Emission ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Ph Probe

We report a red-emission fluorescent pH probe (RML) for visual monitoring of the lysosomal pH changes during autophagy in living cells.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe

Talanta ◽  
2021 ◽  
Vol 228 ◽  
pp. 122184
Author(s):  
Qingfeng Xia ◽  
Shumin Feng ◽  
Jiaxin Hong ◽  
Guoqiang Feng
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Stress ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Real Time Tracking ◽  
Emissive Probe

Differentiation of Viable and Dead Escherichia coli O157:H7 Cells on and in Apple Structures and Tissues following Chlorine Treatment

2002 ◽  
Vol 65 (2) ◽  
pp. 251-259 ◽  
Author(s):  
SCOTT L. BURNETT ◽  
LARRY R. BEUCHAT
Keyword(s):  
Escherichia Coli ◽  
Live Cells ◽  
Confocal Scanning Laser Microscopy ◽  
Escherichia Coli O157 ◽  
Tissue Samples ◽  
E Coli ◽  
Sytox Green ◽  
Viable Cells ◽  
Nucleic Acid Stain ◽  
Protective Structures

Confocal scanning laser microscopy (CSLM) was used to differentiate viable and nonviable cells of Escherichia coli O157:H7 on and in raw apple tissues following treatment with water and 200 or 2,000 ppm active chlorine solution. Whole unwaxed Red Delicious cultivar apples at 25°C were inoculated by dipping in a suspension of E. coli O157:H7 (8.48 log10 CFU/ml) at 4°C, followed by treatment in water or chlorine solution at 21°C for 2 min. The dead cells on and in apples were distinguished from live cells by treating tissue samples with SYTOX green nucleic acid stain. Viable and dead cells were then labeled with an antibody conjugated with a fluorescent dye (Alexa Fluor 594). The percentage of viable cells on the apple surface, as well as at various depths in surface and internal structures, was determined. The mean percentages of viable cells located at the sites after treatment with water or chlorinated water were in the following order, which also reflects the order of protection against inactivation: floral tube wall (20.5%) > lenticels (15.0%) > damaged cuticle surrounding puncture wounds (13.0%) > intact cuticle (8.1%). The location of viable cells within tissues was dependent on the structure. Except for lenticels, the percentage of viable cells increased as depth into the CSLM stacks increased, indicating that cells attached to subsurface structures were better protected against inactivation with chlorine than were cells located on exposed surfaces. Further research is warranted to investigate the efficacy of other chemical sanitizers, as well as that of surfactants and solvents in combination with sanitizers, in removing or killing E. coli O157:H7 lodged in protective structures on the surface and within tissues of apples.

A tumor-targeting and lysosome-specific two-photon fluorescent probe for imaging pH changes in living cells

2017 ◽  
Vol 5 (5) ◽  
pp. 988-995 ◽  
Author(s):  
Baoli Dong ◽  
Xuezhen Song ◽  
Xiuqi Kong ◽  
Chao Wang ◽  
Nan Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Tumor Targeting ◽  
Living Cells ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Two Photon

Lysosomal pH is closely related to the metastasis and apoptosis of cancer cells.

Two-photon fluorescence visualization of lysosomal pH changes during mitophagy and cell apoptosis

Talanta ◽  
2020 ◽  
Vol 209 ◽  
pp. 120549 ◽  
Author(s):  
Yaqi Dong ◽  
Haibin Xiao ◽  
Lingbao Xing ◽  
Chuanchen Wu ◽  
Jin Zhou ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

pH-Sensitive Capsules as Intracellular Optical Reporters for Monitoring Lysosomal pH Changes Upon Stimulation

Small ◽  
2012 ◽  
Vol 8 (6) ◽  
pp. 943-948 ◽  
Author(s):  
Pilar RiveraGil ◽  
Moritz Nazarenus ◽  
Sumaira Ashraf ◽  
Wolfgang J. Parak
Keyword(s):  
Ph Sensitive ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Optical Reporters
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