Modulation of human mesenchymal stem cell survival on electrospun mesh with co-immobilized epithelial growth factor and gelatin

RSC Advances ◽  
2015 ◽  
Vol 5 (69) ◽  
pp. 55948-55956 ◽  
Author(s):  
Young Min Shin ◽  
Jong-Young Lim ◽  
Jong-Seok Park ◽  
Hui-Jeong Gwon ◽  
Sung In Jeong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Cell Survival ◽  
Human Mesenchymal Stem Cell ◽  
Epithelial Growth Factor ◽  
Stem Cell Survival ◽  
Epithelial Growth

Co-immobilization of EGF and gelatin on a fibrous mesh promotes spreading and viability of hMSC, and coupled EGF involves involucrin expression and procollagen secretion, indicating trans-differentiation to keratinocyte-like cell.

Enhanced human mesenchymal stem cell survival under oxidative stress by overexpression of secreted frizzled-related protein 2 gene

2014 ◽  
Vol 94 (2) ◽  
pp. 319-327 ◽  
Author(s):  
Kanjana Pomduk ◽  
Pakpoom Kheolamai ◽  
Yaowalak U-Pratya ◽  
Methichit Wattanapanitch ◽  
Nuttha Klincumhom ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Survival ◽  
Human Mesenchymal Stem Cell ◽  
Related Protein ◽  
Stem Cell Survival

scholarly journals Physoxia alters human mesenchymal stem cell secretome

2021 ◽  
Vol 12 ◽  
pp. 204173142110561
Author(s):  
Marwan M Merkhan ◽  
Matthew T Shephard ◽  
Nicholas R Forsyth
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Therapeutic Potential ◽  
Interleukin 2 ◽  
Human Mesenchymal Stem Cell ◽  
Total Protein Content ◽  
Necrosis Factor Alpha ◽  
Oxygen Conditions ◽  
Endothelial Growth Factor

The human mesenchymal stem cell (hMSC) secretome has pleiotropic effects which underpin their therapeutic potential. hMSC serum-free conditioned media (SFCM) has been determined to contain a variety of cytokines with roles in regeneration and suppression of inflammation. Physiological oxygen (physoxia) has been demonstrated to impact upon a number of facets of hMSC biology and we hypothesized that the secretome would be similarly modified. We tested a range of oxygen conditions; 21% O2 (air oxygen (AO)), 2% O2 (intermittent hypoxia (IH)) and 2% O2 Workstation (physoxia (P)) to evaluate their effect on hMSC secretome profiles. Total protein content of secretome was upregulated in IH and P (>3 fold vs AO) and IH (>1 fold vs P). Focused cytokine profiling indicated global upregulation in IH of all 31 biomolecules tested in comparison to AO and P with basic-nerve growth factor (bNGF) and granulocyte colony-stimulating factor (GCSF) (>3 fold vs AO) and bNGF and Rantes (>3 fold vs P) of note. Similarly, upregulation of interferon gamma-induced protein 10 (IP10) was noted in P (>3 fold vs AO). Interleukin-2 (IL2) and Rantes (in AO and P) and adiponectin, IL17a, and epidermal growth factor (EGF) (in AO only) were entirely absent or below detection limits. Quantitative analysis validated the pattern of IH-induced upregulation in vascular endothelial growth factor (VEGF), placental growth factor-1 (PIGF1), Tumor necrosis factor alpha (TNFa), IL2, IL4, and IL10 when compared to AO and P. In summary, modulation of environmental oxygen alters both secretome concentration and composition. This consideration will likely impact on delivering improved mechanistic understanding and potency effects of hMSC-based therapeutics.

scholarly journals Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation

2017 ◽  
Vol 32 (7) ◽  
pp. 906-919 ◽  
Author(s):  
Dhanak Gupta ◽  
David M Grant ◽  
Kazi M Zakir Hossain ◽  
Ifty Ahmed ◽  
Virginie Sottile
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Survival ◽  
Osteogenic Differentiation ◽  
Stem Cell Survival

scholarly journals P033 Drugs used in the treatment of fistulae in Crohn's disease preserve mesenchymal stem cell survival

2012 ◽  
Vol 6 ◽  
pp. S23-S24 ◽  
Author(s):  
L. Meran ◽  
H. Rashidi ◽  
R. Jones ◽  
F. Rose ◽  
K. Shakesheff ◽  
...  
Keyword(s):  
Stem Cell ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Mesenchymal Stem Cell ◽  
Cell Survival ◽  
Stem Cell Survival
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