scholarly journals Fabrication of rigid microstructures with thiol-ene-based soft lithography for continuous-flow cell lysis

2014 ◽  
Vol 8 (5) ◽  
pp. 056503 ◽  
Author(s):  
Jeffrey M. Burke ◽  
Kunal R. Pandit ◽  
John P. Goertz ◽  
Ian M. White
Keyword(s):  
Continuous Flow ◽  
Soft Lithography ◽  
Cell Lysis ◽  
Flow Cell

Bead beating-based continuous flow cell lysis in a microfluidic device

RSC Advances ◽  
2015 ◽  
Vol 5 (29) ◽  
pp. 22350-22355 ◽  
Author(s):  
A. Berasaluce ◽  
L. Matthys ◽  
J. Mujika ◽  
M. Antoñana-Díez ◽  
A. Valero ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Previous Treatment ◽  
Cell Lysis ◽  
Flow Cell ◽  
Bead Beating

This paper describes a bead beating-based miniaturized cell lysis device that works in continuous flow allowing the analysis of large volumes of samples without previous treatment.

Monitoring of Microwave-Assisted Acid-Catalyzed Transesterification for Biodiesel Production Using FT-NIR Spectroscopy with Continuous-Flow Cell

2015 ◽  
Vol 3 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Evandro L. Dall'Oglio ◽  
Paulo T. de Sousa ◽  
Leonardo Gomes de Vasconcelos ◽  
Carlos Adriano Parizotto ◽  
Ewerton Ferreira Barros ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Nir Spectroscopy ◽  
Flow Cell ◽  
Biodiesel Production ◽  
Microwave Assisted ◽  
Acid Catalyzed

scholarly journals Effects of Hydrocortisone on the Yield and Bactericidal Function of Granulocytes Collected by Continuous-flow Centrifugation

Blood ◽  
1974 ◽  
Vol 44 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Mamoru Shoji ◽  
William R. Vogler
Keyword(s):  
Single Dose ◽  
Flow Rate ◽  
Continuous Flow ◽  
Treated Group ◽  
Flow Cell ◽  
Controlled Study ◽  
Single Intravenous Injection ◽  
Cell Separator ◽  
Bactericidal Function

Abstract The usefulness of granulocyte transfusions is in part dependent upon the number of granulocytes transfused. The invention of the continuous-flow cell separator has made it possible to obtain granulocytes from normal donors. Efforts to improve the yield are under study. This controlled study was undertaken to determine the effect of a single dose of hydrocortisone on granulocyte yield from volunteer donors and on granulocyte bactericidal function. Twenty-two normal volunteers were randomized between no therapy or a single intravenous injection of 120 mg/sq m of hydrocortisone 2 hr prior to initiation of a 4-hr leukapheresis using the Aminco cell separator operated at 750 rpm and a flow rate of 41 ml/min. Significant increases in granulocyte yield and reductions in lymphocyte and monocyte yields were obtained in the hydrocortisone-treated group. Granulocytes from each group were equally effective in the phagocytosis of yeast particles and in vitro bactericidal activity.

Railing cells along 3D microelectrode tracks for continuous-flow dielectrophoretic sorting

Lab on a Chip ◽  
2018 ◽  
Vol 18 (24) ◽  
pp. 3760-3769 ◽  
Author(s):  
Xiaoxing Xing ◽  
Chun Ning Ng ◽  
Ming Lok Chau ◽  
Levent Yobas
Keyword(s):  
Cell Sorting ◽  
Continuous Flow ◽  
Flow Cell ◽  
High Flow ◽  
Hydrodynamic Drag ◽  
Flow Rates

Continuous-flow cell sorting at considerably high flow rates by railing cells along electrode tracks based on dielectrophoresis and hydrodynamic drag.

scholarly journals Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  
Keyword(s):  
Whole Blood ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Single Stage ◽  
Flow Rates ◽  
Blood Separation ◽  
Final Yield ◽  
Component Extraction

Abstract A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

A new principle of continuous flow cell cultivation

PROTOPLASMA ◽  
1986 ◽  
Vol 132 (1-2) ◽  
pp. 99-106 ◽  
Author(s):  
J. Larsen ◽  
J. R. Nilsson
Keyword(s):  
Continuous Flow ◽  
Flow Cell ◽  
Cell Cultivation
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