Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown

Nanoscale ◽  
2015 ◽  
Vol 7 (48) ◽  
pp. 20625-20634 ◽  
Author(s):  
Johans J. Fakhoury ◽  
Thomas G. Edwardson ◽  
Justin W. Conway ◽  
Tuan Trinh ◽  
Farhad Khan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Gene Knockdown ◽  
Polymer Micelles ◽  
Polymer Conjugates ◽  
Efficient Gene

Therapeutic nucleic acid polymer conjugates are powerful molecules for shutting down protein expression.

scholarly journals Correction: Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown

Nanoscale ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 10453-10453
Author(s):  
Johans J. Fakhoury ◽  
Thomas G. Edwardson ◽  
Justin W. Conway ◽  
Tuan Trinh ◽  
Farhad Khan ◽  
...  
Keyword(s):  
Gene Knockdown ◽  
Polymer Micelles ◽  
Efficient Gene

Composite liposome-PEI/nucleic acid lipopolyplexes for safe and efficient gene delivery and gene knockdown

2017 ◽  
Vol 158 ◽  
pp. 93-101 ◽  
Author(s):  
Shashank Reddy Pinnapireddy ◽  
Lili Duse ◽  
Boris Strehlow ◽  
Jens Schäfer ◽  
Udo Bakowsky
Keyword(s):  
Nucleic Acid ◽  
Gene Delivery ◽  
Gene Knockdown ◽  
Efficient Gene

scholarly journals Targeted gene knockdown in zebrafish using negatively charged peptide nucleic acid mimics

2003 ◽  
Vol 228 (3) ◽  
pp. 405-413 ◽  
Author(s):  
Karen A. Urtishak ◽  
Michael Choob ◽  
Xiaobing Tian ◽  
Nitzan Sternheim ◽  
William S. Talbot ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gene Knockdown

Nucleic acid quality and protein expression in sodium alginate FFPE cell blocks prepared under different formalin fixing conditions

2021 ◽  
Vol 60 (1) ◽  
pp. 15-21
Author(s):  
Kazuya MURATA ◽  
Akihiko KAWAHARA ◽  
Hideyuki ABE ◽  
Yorihiko TAKASE ◽  
Tomoko YOSHIDA ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Sodium Alginate

scholarly journals Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air–Blood Barrier Crossing

2020 ◽  
Vol 12 (27) ◽  
pp. 30095-30111
Author(s):  
Alexander Taschauer ◽  
Wolfram Polzer ◽  
Stefan Pöschl ◽  
Slavica Metz ◽  
Nathalie Tepe ◽  
...  
Keyword(s):  
Gene Knockdown ◽  
Barrier Crossing ◽  
Efficient Gene

Design of a Targeted Peptide Nucleic Acid Prodrug To Inhibit Hepatic Human Microsomal Triglyceride Transfer Protein Expression in Hepatocytes†

2002 ◽  
Vol 13 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Erik A. L. Biessen ◽  
Karen Sliedregt-Bol ◽  
Peter A. Chr. 'T Hoen ◽  
Perry Prince ◽  
Erica Van der Bilt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Peptide Nucleic Acid ◽  
Microsomal Triglyceride Transfer Protein ◽  
Transfer Protein

scholarly journals Correlation of mRNA delivery timing and protein expression in lipid-based transfection

2019 ◽  
Author(s):  
A. Reiser ◽  
D. Woschée ◽  
N. Mehrotra ◽  
R. Krzysztoń ◽  
H. H. Strey ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Single Cell ◽  
Serum Protein ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Nucleic Acid Delivery ◽  
Delivery Times ◽  
Time Courses ◽  
Endosomal Release

AbstractNon-viral gene delivery is constrained by the dwell time that most synthetic nucleic acid nanocarriers spend inside endosomal compartments. In order to overcome this endosomal-release bottleneck, methods are required that measure nanocarrier uptake kinetics and transfection efficiency simultaneously. Here, we employ live-cell imaging on single-cell arrays (LISCA) to study the delivery-time distribution of lipid-based mRNA complexes under varied serum conditions. By fitting a translation-maturation model to hundreds of individual eGFP reporter fluorescence time courses, the protein expression onset times and the expression rates after transfection are determined. Using this approach, we find that delivery timing and protein expression rates are not intrinsically correlated at the single-cell level, even though population-averaged values of both parameters conjointly change as a function of increasing external serum protein fraction. Lipofectamine mediated delivery showed decreased transfection efficiency and longer delivery times with increasing serum protein concentration. This is in contrast to ionizable lipid nanoparticles (LNPs) mediated transfer, which showed increased efficiency and faster uptake in the presence of serum. In conclusion, the interdependences of single-cell expression rates and onset timing provide additional clues on uptake and release mechanisms, which are useful for improving nucleic acid delivery.

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