Toehold‐Mediated Strand Displacement in a Triplex Forming Nucleic Acid Clamp for Reversible Regulation of Polymerase Activity and Protein Expression

2019 ◽  
Vol 25 (53) ◽  
pp. 12303-12307
Author(s):  
Thuy J. D. Nguyen ◽  
Ilenia Manuguerra ◽  
Vipin Kumar ◽  
Kurt V. Gothelf
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Polymerase Activity ◽  
Strand Displacement

scholarly journals Handhold-Mediated Strand Displacement: A Nucleic Acid Based Mechanism for Generating Far-from-Equilibrium Assemblies through Templated Reactions

ACS Nano ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 3272-3283
Author(s):  
Javier Cabello-Garcia ◽  
Wooli Bae ◽  
Guy-Bart V. Stan ◽  
Thomas E. Ouldridge
Keyword(s):  
Nucleic Acid ◽  
Strand Displacement ◽  
Far From Equilibrium

scholarly journals Nucleic Acid Strand Displacement with Synthetic mRNA Inputs in Living Mammalian Cells

2018 ◽  
Vol 7 (12) ◽  
pp. 2737-2741 ◽  
Author(s):  
Gourab Chatterjee ◽  
Yuan-Jyue Chen ◽  
Georg Seelig
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Strand Displacement ◽  
Synthetic Mrna

scholarly journals Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions

2020 ◽  
Vol 48 (20) ◽  
pp. 11773-11784
Author(s):  
Jiao Lin ◽  
Yan Liu ◽  
Peidong Lai ◽  
Huixia Ye ◽  
Liang Xu
Keyword(s):  
Nucleic Acid ◽  
Genetic Regulation ◽  
Binding Activity ◽  
Strand Displacement ◽  
Genetic Circuits ◽  
Reporter System ◽  
Gene Expressions ◽  
Guide Rna ◽  
Naturally Occurring ◽  
Novel Approach

Abstract A variety of nanodevices developed for nucleic acid computation provide great opportunities to construct versatile synthetic circuits for manipulation of gene expressions. In our study, by employing a two-hairpin mediated nucleic acid strand displacement as a processing joint for conditional guide RNA, we aim to build artificial connections between naturally occurring RNA expressions through programmable CRISPR/Cas9 function. This two-hairpin joint possesses a sequence-switching machinery, in which a random trigger strand can be processed to release an unconstrained sequence-independent strand and consequently activate the self-inhibitory guide RNA for conditional gene regulation. This intermediate processor was characterized by the fluorescence reporter system and applied for regulation of the CRISPR/Cas9 binding activity. Using plasmids to generate this sequence-switching machinery in situ, we achieved the autonomous genetic regulation of endogenous RNA expressions controlled by other unrelated endogenous RNAs in both E. coli and human cells. Unlike previously reported strand-displacement genetic circuits, this advanced nucleic acid nanomachine provides a novel approach that can establish regulatory connections between naturally occurring endogenous RNAs. In addition to CRISPR systems, we anticipate this two-hairpin machine can serve as a general processing joint for wide applications in the development of other RNA-based genetic circuits.

scholarly journals A nucleic acid strand displacement system for the multiplexed detection of tuberculosis-specific mRNA using quantum dots

Nanoscale ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 10087-10095 ◽  
Author(s):  
H. D. Gliddon ◽  
P. D. Howes ◽  
M. Kaforou ◽  
M. Levin ◽  
M. M. Stevens
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Strand Displacement ◽  
Multiplexed Detection ◽  
Mrna Detection ◽  
Dna Strand Displacement ◽  
Displacement System ◽  
Dna Strand ◽  
Specific Mrna

On the development of a novel multiplexed assay for Tuberculosis-specific mRNA detection using DNA strand displacement and quantum dots.

Nucleic acid quality and protein expression in sodium alginate FFPE cell blocks prepared under different formalin fixing conditions

2021 ◽  
Vol 60 (1) ◽  
pp. 15-21
Author(s):  
Kazuya MURATA ◽  
Akihiko KAWAHARA ◽  
Hideyuki ABE ◽  
Yorihiko TAKASE ◽  
Tomoko YOSHIDA ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Sodium Alginate

scholarly journals Mismatches Improve the Performance of Strand-Displacement Nucleic Acid Circuits

2014 ◽  
Vol 126 (7) ◽  
pp. 1876-1879 ◽  
Author(s):  
Yu Sherry Jiang ◽  
Sanchita Bhadra ◽  
Bingling Li ◽  
Andrew D. Ellington
Keyword(s):  
Nucleic Acid ◽  
Strand Displacement

Design of a Targeted Peptide Nucleic Acid Prodrug To Inhibit Hepatic Human Microsomal Triglyceride Transfer Protein Expression in Hepatocytes†

2002 ◽  
Vol 13 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Erik A. L. Biessen ◽  
Karen Sliedregt-Bol ◽  
Peter A. Chr. 'T Hoen ◽  
Perry Prince ◽  
Erica Van der Bilt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Peptide Nucleic Acid ◽  
Microsomal Triglyceride Transfer Protein ◽  
Transfer Protein
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