scholarly journals Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air–Blood Barrier Crossing

2020 ◽  
Vol 12 (27) ◽  
pp. 30095-30111
Author(s):  
Alexander Taschauer ◽  
Wolfram Polzer ◽  
Stefan Pöschl ◽  
Slavica Metz ◽  
Nathalie Tepe ◽  
...  
Keyword(s):  
Gene Knockdown ◽  
Barrier Crossing ◽  
Efficient Gene

Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown

Nanoscale ◽  
2015 ◽  
Vol 7 (48) ◽  
pp. 20625-20634 ◽  
Author(s):  
Johans J. Fakhoury ◽  
Thomas G. Edwardson ◽  
Justin W. Conway ◽  
Tuan Trinh ◽  
Farhad Khan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Expression ◽  
Gene Knockdown ◽  
Polymer Micelles ◽  
Polymer Conjugates ◽  
Efficient Gene

Therapeutic nucleic acid polymer conjugates are powerful molecules for shutting down protein expression.

scholarly journals Correction: Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown

Nanoscale ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 10453-10453
Author(s):  
Johans J. Fakhoury ◽  
Thomas G. Edwardson ◽  
Justin W. Conway ◽  
Tuan Trinh ◽  
Farhad Khan ◽  
...  
Keyword(s):  
Gene Knockdown ◽  
Polymer Micelles ◽  
Efficient Gene

scholarly journals Efficient Gene Knockdown in Mouse Oocytes through Peptide Nanoparticle-Mediated SiRNA Transfection

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150462 ◽  
Author(s):  
Zhen Jin ◽  
Ruichao Li ◽  
Chunxiang Zhou ◽  
Liya Shi ◽  
Xiaolan Zhang ◽  
...  
Keyword(s):  
Gene Knockdown ◽  
Sirna Transfection ◽  
Mouse Oocytes ◽  
Efficient Gene

Calcium mediated formation of phosphorylcholine-based polyplexes for efficient knockdown of epidermal growth factor receptors (EGFR) in HeLa cells

2014 ◽  
Vol 50 (22) ◽  
pp. 2943-2946 ◽  
Author(s):  
Marya Ahmed ◽  
Kazuhiko Ishihara ◽  
Ravin Narain
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Hela Cells ◽  
Growth Factor Receptors ◽  
Gene Knockdown ◽  
Epidermal Growth Factor Receptors ◽  
Efficient Gene ◽  
Epidermal Growth ◽  
Presence And Absence

Calcium mediated complexation of siRNA with phosphorylcholine based polymers for efficient gene knockdown in HeLa cells in the presence and absence of serum.

scholarly journals Gold nanocluster mediated delivery of siRNA to intact plant cells for efficient gene knockdown

2021 ◽  
Author(s):  
Huan Zhang ◽  
Yuhong Cao ◽  
Dawei Xu ◽  
Natalie S. Goh ◽  
Gozde S. Demirer ◽  
...  
Keyword(s):  
Target Genes ◽  
Plant Cell Wall ◽  
Sirna Delivery ◽  
Plant Cells ◽  
Intact Plant ◽  
Gene Knockdown ◽  
Size Exclusion ◽  
Plant Host ◽  
Gold Nanocluster ◽  
Efficient Gene

AbstractRNA interference (RNAi), which involves the delivery of small interfering RNA molecules (siRNA), has been used to validate target genes in plants, to understand and control cellular metabolic pathways, and as a ‘green’ alternative for crop pest tolerance. Conventional siRNA delivery methods such as viruses and Agrobacterium-mediated delivery exhibit limitations in host plant species range and their use can result in uncontrolled DNA integration into the plant host genome. Here, we synthesize polyethyleneimine functionalized gold nanoclusters (PEI-AuNCs) to mediate siRNA delivery into intact plant cells and show these constructs enable efficient gene knockdown. We demonstrate that functionalized AuNCs protect siRNA from RNase degradation and are small enough (~2 nm) to bypass the plant cell wall which exhibits a size exclusion limit of 5-20 nm. These AuNCs in turn enable up to 76.5 ± 5.9% GFP mRNA knockdown efficiency with no cellular toxicity. Our data suggest this simple and biocompatible platform for passive delivery of siRNA into intact plant cells could have broad applications in plant biotechnology.

scholarly journals Electroporation of short hairpin RNAs for rapid and efficient gene knockdown in the starlet sea anemone, Nematostella vectensis

2019 ◽  
Vol 448 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Ahmet Karabulut ◽  
Shuonan He ◽  
Cheng-Yi Chen ◽  
Sean A. McKinney ◽  
Matthew C. Gibson
Keyword(s):  
Sea Anemone ◽  
Gene Knockdown ◽  
Nematostella Vectensis ◽  
Efficient Gene ◽  
Short Hairpin ◽  
Short Hairpin Rnas

Composite liposome-PEI/nucleic acid lipopolyplexes for safe and efficient gene delivery and gene knockdown

2017 ◽  
Vol 158 ◽  
pp. 93-101 ◽  
Author(s):  
Shashank Reddy Pinnapireddy ◽  
Lili Duse ◽  
Boris Strehlow ◽  
Jens Schäfer ◽  
Udo Bakowsky
Keyword(s):  
Nucleic Acid ◽  
Gene Delivery ◽  
Gene Knockdown ◽  
Efficient Gene
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