scholarly journals In vitrocharacterization of a novel C,N-cyclometalated benzimidazole Ru(ii) arene complex: stability, intracellular distribution and binding, effects on organic osmolyte homeostasis and induction of apoptosis

Metallomics ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 885-895 ◽  
Author(s):  
Celina Støving Dam ◽  
Sergio Alejo Perez Henarejos ◽  
Theodosia Tsolakou ◽  
Christian Alexander Segato ◽  
Bente Gammelgaard ◽  
...  
Keyword(s):  
Cisplatin Resistance ◽  
Intracellular Distribution ◽  
Complex Stability ◽  
Organic Osmolyte ◽  
Arene Complex ◽  
Induction Of Apoptosis

Applying a novel protocol for the characterization of metal-based drugs reveals the potential of a new Ru-based compound in overcoming Cisplatin resistance.

A characterization of heam uptake and intracellular distribution by isolated hepatocytes

1985 ◽  
Vol 5 (7) ◽  
pp. 609-614 ◽  
Author(s):  
A. Lodola
Keyword(s):  
Endoplasmic Reticulum ◽  
Specific Binding ◽  
Isolated Hepatocytes ◽  
Intracellular Distribution ◽  
Distribution Data ◽  
Temperature Dependent ◽  
Rat Hepatocyte ◽  
Isolated Rat ◽  
Endoplasmic Reticulum Fraction

The uptake and intracellular distribution of haem by isolated rat hepatocyte suspensions was studied. An increase in cell haem content occurred after a challenge with 5, 10 or 20 μM haem, supplied as methaemalbumin. The rate of haem uptake was temperature dependent; no non-specific binding occurred. Intracellular haem distribution data are consistent with a rapid association of haem with the endoplasmic reticulum fraction prior to its accumulation in the cytosol and at the mitochondrion.

Nucleophilic addition to di- and poly-iron arene complex cations

1996 ◽  
Vol 74 (11) ◽  
pp. 2073-2082 ◽  
Author(s):  
Alaa S. Abd-Ei-Aziz ◽  
Debbie A. Armstrong ◽  
Shelly Bernardin ◽  
Harold M. Hutton
Keyword(s):  
Nucleophilic Addition ◽  
Iron Complexes ◽  
Iron Complex ◽  
Cyanide Anion ◽  
Meta Position ◽  
Arene Complex ◽  
Nmr Techniques ◽  
Cyano Groups ◽  
Predominant Effect

Hydride and cyanide addition to a series of di- and polycyclopentadienyliron arene complex cations with etheric bridges is described. Reaction of the di-iron complexes with sodium borohydride resulted in the formation of a number of adducts.p-Methyl- and o,o-dimethylphenoxybenzene cyclopentadienyliron complexes were used as models in this study to allow for the characterization of the analagous di-iron complexes. The use of HH COSY and CH COSY NMR techniques enabled us to identify the isomeric nature of these adducts. The hydride addition results indicated that the etheric substituent had the predominant effect over the methyl group, leading to a higher addition ratio to the meta-, followed by the ortho-, then the para-positions. It was also clear that in the di-iron system, the hydride addition to each complexed arene ring took place independently. The addition of the cyanide anion to di- and poly-iron arene systems was more selective than that of the hydride anion. Reaction of sodium cyanide with p-methyl- or o-methyl-substituted arene complexes led to the formation of one adduct, with the cyanide being added to the meta position to the etheric bridges. However, cyanide addition to the di-iron complex, with a methyl substituent attached at the meta position of each complexed arene, led to the formation of a mixture of adducts. Cyanide addition to the poly-iron system with p-substituted arenes proved to be very selective, allowing for the formation of one adduct. Oxidative demetallation with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) produced the uncomplexed polyaromatic ethers with cyano groups in a very good yield. Key words: cyclopentadienyliron, arene, nucleophilic addition, hydride, cyanide.

scholarly journals Isolation and characterization of human nuclear and cytosolic multisynthetase complexes and the intracellular distribution of p43/EMAPII

2009 ◽  
Vol 12 (10) ◽  
pp. 2282-2290 ◽  
Author(s):  
Cindy L. Wolfe ◽  
J. Anthony Warrington ◽  
Stanitia Davis ◽  
Sherrina Green ◽  
Mona T. Norcum
Keyword(s):  
Intracellular Distribution ◽  
Isolation And Characterization

scholarly journals Studies on the heparin sulphamidase activity from rat spleen. Intracellular distribution and characterization of the enzyme

1974 ◽  
Vol 139 (3) ◽  
pp. 699-708 ◽  
Author(s):  
Yochanan Friedman ◽  
Charalampos Arsenis
Keyword(s):  
Isoelectric Focusing ◽  
Intracellular Distribution ◽  
Distinct Entity ◽  
Lysosomal Fraction

A sulphamidase activity present in rat spleen capable of hydrolysing N-[35S]sulphated heparin was studied. This activity was associated with the lysosomal fraction. Studies in vivo showed that the rat is capable of significantly desulphating heparin. Lysosomes in all the major tissues can effectively accumulate heparin. The heparin sulphamidase and arylsulphatase activities from rat spleen were separated by isoelectric focusing. Heparin sulphamidase was a distinct entity from all the arylsulphatase activities.

Establishment and characterization of a novel ALL-L3 cell line (BALM-18): induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells

1999 ◽  
Vol 23 (6) ◽  
pp. 559-568 ◽  
Author(s):  
Yoshinobu Matsuo ◽  
Akira Sugimoto ◽  
Akira Harashima ◽  
Chiharu Nishizaki ◽  
Fumihiko Ishimaru ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Bone Marrow Stroma ◽  
Marrow Stroma ◽  
Bone Marrow Stroma Cells ◽  
Induction Of Apoptosis
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