Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Gas Column/Mass Spectrometry Analysis of Bio Oil Treated by Aqueous Two Phase System

2011 ◽  
Vol 236-238 ◽  
pp. 54-58
Author(s):  
Shao Peng Liu ◽  
Zhi Min Zong ◽  
Shan Shan Zhang ◽  
Yue Xu ◽  
Gui Zhen Gong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Spectrometry Analysis ◽  
Bio Oil ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ethanol System

Bio oil aqueous two phase systems were formed with ethanol and the salts of (NH4)2SO4, K2HPO4 and NaH2PO4 in bio oil on the ratio of 10:5:10(by mass). After phase separation, both the upper and the lower layers were analyzed by gas column/mass spectrometry (GC/MS). As a result, both K2HPO4/ethanol and NaH2PO4/ethanol system can enrich phenols and levoglucosan in the upperand lower layers,respectly, while NaH2PO4/ethanol system seldom changes the components of bio oil; the (NH4)2SO4/ethanol system is quit different with the other two and we have detected some components with nitrogen. Hence, NaH2PO4/ethanol is an advantage aqueous two phase system for bio oil treatment.

Passive droplet generation in aqueous two-phase systems with a variable-width microchannel

Soft Matter ◽  
2019 ◽  
Vol 15 (23) ◽  
pp. 4647-4655 ◽  
Author(s):  
Daeho Choi ◽  
Eunjeong Lee ◽  
Sung-Jin Kim ◽  
Minsub Han
Keyword(s):  
Droplet Generation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Passive droplet generation for an aqueous two-phase system (ATPS) was performed with a fracture-based variable microchannel.

Extraction and stability of selected proteins in ionic liquid based aqueous two phase systems

2014 ◽  
Vol 16 (5) ◽  
pp. 2670-2679 ◽  
Author(s):  
Rupali K. Desai ◽  
Mathieu Streefland ◽  
Rene H. Wijffels ◽  
Michel H. M. Eppink
Keyword(s):  
Ionic Liquid ◽  
Aqueous Solutions ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
The Stability

This article discusses the extraction of Rubisco in an ionic liquid (IL) based aqueous two phase system and the stability of proteins in aqueous solutions of IL.

Influence of process variables on extraction of Cefalexin in a novel biocompatible ionic liquid based-aqueous two phase system

2015 ◽  
Vol 17 (1) ◽  
pp. 655-669 ◽  
Author(s):  
Shiva Abdolrahimi ◽  
Bahram Nasernejad ◽  
Gholamreza Pazuki
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Process Variables ◽  
Organic Salts ◽  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase System ◽  
System P ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Despite the fact that ionic liquid-based aqueous two phase systems (ATPSs) have been widely studied for extraction purposes, the adequacy of biodegradable organic salts as salting out agents has been left unexploited.

scholarly journals Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

2003 ◽  
Vol 46 (4) ◽  
pp. 741-750 ◽  
Author(s):  
Xu Yan ◽  
Maria Aparecida Souza ◽  
Marcela Z. R. Pontes ◽  
Michele Vitolo ◽  
Adalberto Pessoa Júnior
Keyword(s):  
Reactive Dyes ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Affinity Ligands ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction ◽  
Commercial Scale ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.

Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 1938-1945 ◽  
Author(s):  
Mackenzie Ruthven ◽  
Kristin Robin Ko ◽  
Rishima Agarwal ◽  
John P. Frampton
Keyword(s):  
Phase Transitions ◽  
Simple Technique ◽  
Rapid Determination ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microscopic Evaluation ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

A simple technique is presented to identify phase-transitions in polymeric aqueous two-phase systems.

scholarly journals Water-in-water droplets by passive microfluidic flow focusing

2021 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Niki Abbasi ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Microfluidic System ◽  
Column Height ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Passive Flow ◽  
Microfluidic Flow ◽  
Droplet Production ◽  
Order Of Magnitude

We present a simple microfluidic system that generates water-in-water, aqueous two phase system (ATPS) droplets, by passive flow focusing. ATPS droplet formation is achieved by applying weak hydrostatic pressures, with liquid-filled pipette tips as fluid columns at the inlets, to introduce low speed flows to the flow focusing junction. To control the size of the droplets, we systematically vary the interfacial tension and viscosity of the ATPS fluids, and adjust the fluid column height at the fluid inlets. The size of the droplets scales with a power-law of the ratio of viscous stresses in the two ATPS phases. Overall, we find a drop size coefficient of variation (CV; i.e. polydispersity) of about 10 %. We also find that when drops form very close to the flow focusing junction, the drops have CV of less than 1 %. Our droplet generation method is easily scalable: we demonstrate a parallel system that generates droplets simultaneously, and improves the droplet production rate by up to one order-of-magnitude. Finally, we show the potential application of our system for encapsulating cells in water-in-water emulsions, by encapsulating microparticles and cells. To the best of our knowledge, our microfluidic technique is the first that forms low interfacial tension ATPS droplets without applying external perturbations. We anticipate that this simple approach will find utility in drug and cell delivery applications because of the all-biocompatible nature of the water-in-water ATPS environment.

Interfacial Tension Effect on Cell Partition in Aqueous Two-Phase Systems

2015 ◽  
Vol 7 (38) ◽  
pp. 21305-21314 ◽  
Author(s):  
Ehsan Atefi ◽  
Ramila Joshi ◽  
Jay Adin Mann ◽  
Hossein Tavana
Keyword(s):  
Interfacial Tension ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Cell Partition

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

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