Interfacial Tension Effect on Cell Partition in Aqueous Two-Phase Systems

2015 ◽  
Vol 7 (38) ◽  
pp. 21305-21314 ◽  
Author(s):  
Ehsan Atefi ◽  
Ramila Joshi ◽  
Jay Adin Mann ◽  
Hossein Tavana
Keyword(s):  
Interfacial Tension ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Cell Partition

Effect of Molecular Weight of Phase Polymers on Partition of Cells in Aqueous Two-Phase Systems

MRS Advances ◽  
2017 ◽  
Vol 2 (45) ◽  
pp. 2415-2426 ◽  
Author(s):  
Ehsan Atefi ◽  
Ramila Joshi ◽  
Hossein Tavana
Keyword(s):  
Molecular Weight ◽  
Interfacial Tension ◽  
Aqueous Environment ◽  
Significant Shift ◽  
Two Phase ◽  
Molecular Weights ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Cell Partition ◽  
Number Of Cells

ABSTRACTDue to their aqueous environment and biocompatible polymers, aqueous two-phase systems (ATPS) provide a mild environment for partition of cells. A comprehensive understanding of cell partition in ATPS will facilitate cell separation and fractionation in ATPS for downstream cell-based analytical applications and various cellular and molecular biology studies. We report the effect of molecular weight of phase polymers on partition of cells between two aqueous phases and their interface. We generate three different ATPS by dissolving polyethylene glycol and dextran of different molecular weights in a standard cell culture medium of fixed composition. After suspending cells in each ATPS, we quantify the number of cells partitioned into each segregated phase and the interface between the two phases. Importantly, we use two-phase solutions of an identical interfacial tension from each ATPS to avoid the effect of interfacial tension on cell partition. Our results indicate that decreasing the molecular weight of one of the phase polymers results in distribution of a greater number of cells toward the phase rich in that polymer. Regardless of molecular weight of polymers used, two-phase solutions made with higher concentrations of polymers cause a significant shift toward cell partition to the interface. This study elucidates the role of polymer molecular weight on cell partition in ATPS and offers formulations for rapid and effective cell partition.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Interfacial Tension of Aqueous Two-Phase Systems Containing Poly(ethylene glycol) and Potassium Phosphate

2012 ◽  
Vol 57 (6) ◽  
pp. 1648-1652 ◽  
Author(s):  
Cássio Carmo de Oliveira ◽  
Jane Sélia dos Reis Coimbra ◽  
Abraham Damian Giraldo Zuniga ◽  
João Paulo Martins ◽  
Antonio Marcos de Oliveira Siqueira
Keyword(s):  
Interfacial Tension ◽  
Ethylene Glycol ◽  
Potassium Phosphate ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene

Interfacial Tension and Viscosity for Poly(ethylene glycol) + Maltodextrin Aqueous Two-Phase Systems

2006 ◽  
Vol 51 (3) ◽  
pp. 1144-1147 ◽  
Author(s):  
Abraham Damian Giraldo-Zuniga ◽  
Jane Sélia dos Reis Coimbra ◽  
Daniela Aparecida Arquete ◽  
Luís Antonio Minim ◽  
Luís Henrique Mendes da Silva ◽  
...  
Keyword(s):  
Interfacial Tension ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene

scholarly journals The dripping-to-jetting transition in a co-axial flow of aqueous two-phase systems with low interfacial tension

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3287-3292 ◽  
Author(s):  
Sze Yi Mak ◽  
Youchuang Chao ◽  
Ho Cheung Shum
Keyword(s):  
Interfacial Tension ◽  
Axial Flow ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Low Interfacial Tension ◽  
Phase Systems

The dripping-to-jetting transition of co-axial flow with high interfacial tension has been extensively studied; however, little is known about this with low interfacial tension.

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