Extraction and stability of selected proteins in ionic liquid based aqueous two phase systems

2014 ◽  
Vol 16 (5) ◽  
pp. 2670-2679 ◽  
Author(s):  
Rupali K. Desai ◽  
Mathieu Streefland ◽  
Rene H. Wijffels ◽  
Michel H. M. Eppink
Keyword(s):  
Ionic Liquid ◽  
Aqueous Solutions ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
The Stability

This article discusses the extraction of Rubisco in an ionic liquid (IL) based aqueous two phase system and the stability of proteins in aqueous solutions of IL.

Influence of process variables on extraction of Cefalexin in a novel biocompatible ionic liquid based-aqueous two phase system

2015 ◽  
Vol 17 (1) ◽  
pp. 655-669 ◽  
Author(s):  
Shiva Abdolrahimi ◽  
Bahram Nasernejad ◽  
Gholamreza Pazuki
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Process Variables ◽  
Organic Salts ◽  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase System ◽  
System P ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Despite the fact that ionic liquid-based aqueous two phase systems (ATPSs) have been widely studied for extraction purposes, the adequacy of biodegradable organic salts as salting out agents has been left unexploited.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Gas Column/Mass Spectrometry Analysis of Bio Oil Treated by Aqueous Two Phase System

2011 ◽  
Vol 236-238 ◽  
pp. 54-58
Author(s):  
Shao Peng Liu ◽  
Zhi Min Zong ◽  
Shan Shan Zhang ◽  
Yue Xu ◽  
Gui Zhen Gong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Spectrometry Analysis ◽  
Bio Oil ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ethanol System

Bio oil aqueous two phase systems were formed with ethanol and the salts of (NH4)2SO4, K2HPO4 and NaH2PO4 in bio oil on the ratio of 10:5:10(by mass). After phase separation, both the upper and the lower layers were analyzed by gas column/mass spectrometry (GC/MS). As a result, both K2HPO4/ethanol and NaH2PO4/ethanol system can enrich phenols and levoglucosan in the upperand lower layers,respectly, while NaH2PO4/ethanol system seldom changes the components of bio oil; the (NH4)2SO4/ethanol system is quit different with the other two and we have detected some components with nitrogen. Hence, NaH2PO4/ethanol is an advantage aqueous two phase system for bio oil treatment.

Passive droplet generation in aqueous two-phase systems with a variable-width microchannel

Soft Matter ◽  
2019 ◽  
Vol 15 (23) ◽  
pp. 4647-4655 ◽  
Author(s):  
Daeho Choi ◽  
Eunjeong Lee ◽  
Sung-Jin Kim ◽  
Minsub Han
Keyword(s):  
Droplet Generation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Passive droplet generation for an aqueous two-phase system (ATPS) was performed with a fracture-based variable microchannel.

scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

scholarly journals Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

2003 ◽  
Vol 46 (4) ◽  
pp. 741-750 ◽  
Author(s):  
Xu Yan ◽  
Maria Aparecida Souza ◽  
Marcela Z. R. Pontes ◽  
Michele Vitolo ◽  
Adalberto Pessoa Júnior
Keyword(s):  
Reactive Dyes ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Affinity Ligands ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction ◽  
Commercial Scale ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.

scholarly journals Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiwen Bai ◽  
Yanhong Chao ◽  
Meiling Zhang ◽  
Changri Han ◽  
Wenshuai Zhu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Efficiency ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 1938-1945 ◽  
Author(s):  
Mackenzie Ruthven ◽  
Kristin Robin Ko ◽  
Rishima Agarwal ◽  
John P. Frampton
Keyword(s):  
Phase Transitions ◽  
Simple Technique ◽  
Rapid Determination ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microscopic Evaluation ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

A simple technique is presented to identify phase-transitions in polymeric aqueous two-phase systems.

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